Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   VUK78_RS12180 Genome accession   NZ_CP150467
Coordinates   341217..342233 (+) Length   338 a.a.
NCBI ID   WP_026138410.1    Uniprot ID   -
Organism   Vibrio harveyi strain NH-LM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 336217..347233
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK78_RS12165 (VUK78_12165) - 336590..337318 (-) 729 WP_005451494.1 redoxin family protein -
  VUK78_RS12170 (VUK78_12170) oxyR 337493..338401 (+) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  VUK78_RS12175 (VUK78_12175) - 338468..341038 (-) 2571 WP_050937527.1 PBP1A family penicillin-binding protein -
  VUK78_RS12180 (VUK78_12180) pilM 341217..342233 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  VUK78_RS12185 (VUK78_12185) pilN 342217..342792 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  VUK78_RS12190 (VUK78_12190) pilO 342785..343378 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  VUK78_RS12195 (VUK78_12195) pilP 343368..343883 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  VUK78_RS12200 (VUK78_12200) pilQ 343918..345675 (+) 1758 WP_195724146.1 type IV pilus secretin PilQ family protein Machinery gene
  VUK78_RS12205 (VUK78_12205) aroK 345865..346383 (+) 519 WP_005451507.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38053.43 Da        Isoelectric Point: 6.4206

>NTDB_id=970402 VUK78_RS12180 WP_026138410.1 341217..342233(+) (pilM) [Vibrio harveyi strain NH-LM1]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=970402 VUK78_RS12180 WP_026138410.1 341217..342233(+) (pilM) [Vibrio harveyi strain NH-LM1]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAACGCT
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGCGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGAACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.828

100

0.908
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment