Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OO774_RS02790 Genome accession   NZ_AP026763
Coordinates   571121..572806 (-) Length   561 a.a.
NCBI ID   WP_264904468.1    Uniprot ID   -
Organism   Vibrio sp. STUT-A11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 566121..577806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO774_RS02760 (VspSTUT11_04640) rplS 566268..566621 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  OO774_RS02765 (VspSTUT11_04650) yacG 567267..567461 (-) 195 WP_264904458.1 DNA gyrase inhibitor YacG -
  OO774_RS02770 (VspSTUT11_04660) zapD 567548..568288 (-) 741 WP_264904461.1 cell division protein ZapD -
  OO774_RS02775 (VspSTUT11_04670) coaE 568315..568929 (-) 615 WP_264904463.1 dephospho-CoA kinase -
  OO774_RS02780 (VspSTUT11_04680) pilD 568930..569799 (-) 870 WP_264904464.1 A24 family peptidase Machinery gene
  OO774_RS02785 (VspSTUT11_04690) pilC 569867..571090 (-) 1224 WP_264904466.1 type II secretion system F family protein Machinery gene
  OO774_RS02790 (VspSTUT11_04700) pilB 571121..572806 (-) 1686 WP_264904468.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OO774_RS02795 (VspSTUT11_04710) pilA 572810..573259 (-) 450 WP_264904470.1 pilin Machinery gene
  OO774_RS02800 (VspSTUT11_04720) nadC 573518..574405 (-) 888 WP_264904472.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OO774_RS02805 (VspSTUT11_04730) ampD 574498..575043 (+) 546 WP_264904474.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OO774_RS02810 (VspSTUT11_04740) pdhR 575454..576221 (+) 768 WP_264904476.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62093.84 Da        Isoelectric Point: 5.6441

>NTDB_id=96806 OO774_RS02790 WP_264904468.1 571121..572806(-) (pilB) [Vibrio sp. STUT-A11]
MERNLSTILRQAGLLNAAQEESLIEQVNASGTSMPEALVSSGLFTSSEFAEHLSSLFGLNRPELSQYEYASLCQQLGLRE
LITRHYALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVDLVIADFAELDAAIRRLYGRSLGQEKVGLKEINQEELAN
LVDVGADEIDNIEDLSLDDSPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLTNMGVESFNLASSLSLIIAQRLARKLCPHCKKPQEVTTALQHIGIQATDQ
IYQANPNGCNHCTHGYSGRTGIYEVMRFDETLSEALIKGASVHQLEKLAIHNGMSTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=96806 OO774_RS02790 WP_264904468.1 571121..572806(-) (pilB) [Vibrio sp. STUT-A11]
ATGGAGCGCAACCTCTCAACAATTCTTCGCCAAGCGGGCTTACTCAATGCAGCCCAAGAAGAGTCGTTAATAGAGCAAGT
CAACGCTTCTGGTACTTCGATGCCGGAAGCGTTAGTGAGTTCTGGATTATTTACATCAAGCGAGTTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCGGCCTGAGTTATCTCAATATGAGTACGCTTCTCTTTGTCAACAACTTGGTCTTAGGGAG
TTAATCACACGTCATTACGCGCTCCCACTCCACCGCACGCCTTCAACGTTATTATTAGCGGTTGCAGACCCCACGAATCA
ACAAGCTGAAGATGATTTTCGCTTTGCTACCGGATTACAAGTCGACCTTGTCATCGCAGATTTTGCTGAACTTGATGCCG
CGATTCGCCGTTTATACGGTCGCTCTCTTGGCCAGGAAAAAGTAGGCTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATCGAAGACTTAAGCCTGGACGACTCACCGGTTAGTCGCTATATCAA
CCAGATTTTGCTCGACGCGGTGCGTAAAGGCGCATCCGATATCCACTTTGAGCCTTACGAAAAAATGTATCGGGTTCGCC
TACGCTGCGACGGTATTCTAATTGAGACTCAGCAGCCGCCAAGCCATTTAAGTCGGCGCTTATCAGCACGGATCAAAATT
CTGTCCAAACTAGATATTGCCGAAAGGCGTTTGCCACAAGACGGACGTATCAAGCTGAAACTCAATCAAGACACTGCCAT
TGATATGCGAGTGTCCACCTTACCCACCCTGTTCGGAGAAAAGATCGTATTACGACTGCTCGACAGCAGCTCCGCATCAC
TGGATATCGACAAACTAGGCTACAGTGACCAGCAAAAGCAGCTGTATTTAGATGCTCTTCGTCGCCCACAAGGCATGATT
TTAATGACTGGCCCAACTGGGAGCGGCAAAACCGTCTCGTTGTACACAGGGCTGAGCATACTCAATAAACCAGAGATAAA
TATATCTACGGCTGAAGACCCGGTCGAAATCAACCTCTCCGGAATTAATCAGGTTCAGGTTCAGCCTAAGATAGGCTTTG
GTTTCGCTGAGGCACTGCGCTCTTTCCTGCGTCAAGACCCTGATGTGGTCATGGTGGGAGAAATCCGCGATCTGGACACG
GCAGAAATTGCGATTAAGGCATCACAAACCGGACACCTAGTCTTGTCGACGCTCCACACCAATTCAGCCGCGGAAACCGT
TATCCGCCTAACGAATATGGGCGTTGAAAGCTTTAACCTCGCCTCTTCGCTCAGCCTTATTATTGCCCAGCGCTTAGCCC
GTAAGCTTTGCCCGCACTGTAAAAAGCCTCAAGAGGTTACCACCGCATTACAACATATTGGCATTCAAGCAACTGACCAG
ATTTATCAAGCTAACCCCAATGGCTGCAACCACTGTACACACGGCTATTCGGGACGAACTGGCATCTATGAGGTGATGCG
TTTCGATGAAACCCTATCCGAAGCACTGATTAAAGGTGCCTCTGTACATCAACTCGAAAAACTCGCCATCCATAACGGGA
TGAGCACACTACAAATGTCAGGTATAGAAAAGCTTAAGCAAGGCGTCACAAGCTTTAGCGAACTGCAGCGCGTGCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

91.444

100

0.914
  pilB Vibrio campbellii strain DS40M4

87.701

100

0.877
  pilB Vibrio cholerae strain A1552

73.843

100

0.74
  pilB Acinetobacter baumannii D1279779

49.634

97.326

0.483
  pilB Legionella pneumophila strain ERS1305867

48.022

99.109

0.476
  pilB Acinetobacter baylyi ADP1

47.756

99.287

0.474
  pilF Neisseria gonorrhoeae MS11

44.326

100

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

37.129

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.324

100

0.378
  pilF Thermus thermophilus HB27

37.037

100

0.374

Multiple sequence alignment