Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AADG46_RS09835 Genome accession   NZ_AP028804
Coordinates   2176145..2176549 (+) Length   134 a.a.
NCBI ID   WP_000649343.1    Uniprot ID   -
Organism   Vibrio cholerae strain VN152     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171145..2181549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG46_RS09810 (VNVC001_18600) dsbC 2171705..2172457 (-) 753 WP_085603849.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  AADG46_RS09815 (VNVC001_18610) xerD 2172474..2173382 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  AADG46_RS09820 (VNVC001_18620) fldB 2173520..2174038 (+) 519 WP_000690112.1 flavodoxin FldB -
  AADG46_RS09825 (VNVC001_18630) ampD 2174169..2174714 (-) 546 WP_340701785.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AADG46_RS09830 (VNVC001_18640) nadC 2175010..2175900 (+) 891 WP_114743264.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AADG46_RS09835 (VNVC001_18650) pilA 2176145..2176549 (+) 405 WP_000649343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AADG46_RS09840 (VNVC001_18660) pilB 2176556..2178244 (+) 1689 WP_148503289.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AADG46_RS09845 (VNVC001_18670) pilC 2178287..2179513 (+) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  AADG46_RS09850 (VNVC001_18680) pilD 2179571..2180446 (+) 876 WP_000418753.1 A24 family peptidase Machinery gene
  AADG46_RS09855 (VNVC001_18690) coaE 2180443..2181051 (+) 609 WP_000011551.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 134 a.a.        Molecular weight: 13543.58 Da        Isoelectric Point: 9.4492

>NTDB_id=96326 AADG46_RS09835 WP_000649343.1 2176145..2176549(+) (pilA) [Vibrio cholerae strain VN152]
MKAYKNKQQQGFTLIELMIVVAVIGVLAAIAVPQYQKYTQKAALATALATATALKTNYEDYVSMSGAAPTSATDIGATSF
SIGTISVTSGAINVAINKGPGTGSSVILTRTGPLGGWGCQISGAQTGVTINGCP

Nucleotide


Download         Length: 405 bp        

>NTDB_id=96326 AADG46_RS09835 WP_000649343.1 2176145..2176549(+) (pilA) [Vibrio cholerae strain VN152]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCAGTGATTGGTGTGTT
GGCAGCGATTGCGGTGCCTCAATATCAGAAATATACACAAAAAGCAGCTCTTGCTACTGCTTTAGCTACAGCAACTGCAT
TAAAAACAAATTATGAAGACTATGTATCTATGAGTGGTGCAGCTCCCACATCTGCAACAGATATTGGAGCAACGAGCTTC
AGTATAGGAACTATTTCAGTGACTAGTGGTGCTATAAACGTTGCTATAAATAAGGGACCAGGCACTGGCTCATCTGTAAT
TTTAACTAGAACAGGGCCTCTTGGTGGCTGGGGATGCCAAATCTCCGGAGCACAAACTGGAGTAACAATTAACGGTTGCC
CATAG

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

37.013

100

0.425
  pilA Vibrio parahaemolyticus RIMD 2210633

37.838

100

0.418
  pilA Acinetobacter baumannii strain A118

40.441

100

0.41
  pilA Pseudomonas aeruginosa PAK

40.299

100

0.403
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

61.628

64.179

0.396
  pilA Vibrio cholerae strain A1552

61.628

64.179

0.396
  pilA Vibrio cholerae C6706

61.628

64.179

0.396
  pilA/pilA1 Eikenella corrodens VA1

35.374

100

0.388
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.462

97.015

0.373
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.2

93.284

0.366

Multiple sequence alignment