Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WF513_RS14525 Genome accession   NZ_CP149803
Coordinates   3060570..3061787 (+) Length   405 a.a.
NCBI ID   WP_339080100.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3055570..3066787
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF513_RS14505 (WF513_14505) nadC 3056419..3057282 (+) 864 WP_339080096.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WF513_RS14510 (WF513_14510) tfpZ 3057310..3058083 (-) 774 WP_339080097.1 TfpX/TfpZ family type IV pilin accessory protein -
  WF513_RS14515 (WF513_14515) pilA2 3058137..3058640 (-) 504 WP_339080098.1 pilin Machinery gene
  WF513_RS14520 (WF513_14520) pilB 3058863..3060566 (+) 1704 WP_339080099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WF513_RS14525 (WF513_14525) pilC 3060570..3061787 (+) 1218 WP_339080100.1 type II secretion system F family protein Machinery gene
  WF513_RS14530 (WF513_14530) pilD 3061791..3062660 (+) 870 WP_339080101.1 A24 family peptidase Machinery gene
  WF513_RS14535 (WF513_14535) coaE 3063079..3063693 (+) 615 WP_339080102.1 dephospho-CoA kinase -
  WF513_RS14540 (WF513_14540) yacG 3063690..3063890 (+) 201 WP_339080103.1 DNA gyrase inhibitor YacG -
  WF513_RS14545 (WF513_14545) - 3063952..3064167 (-) 216 WP_339080104.1 hypothetical protein -
  WF513_RS14550 (WF513_14550) - 3064272..3064961 (-) 690 WP_339080105.1 energy-coupling factor ABC transporter permease -
  WF513_RS14555 (WF513_14555) - 3065106..3065735 (+) 630 WP_339080106.1 DUF1780 domain-containing protein -
  WF513_RS14560 (WF513_14560) - 3065732..3066226 (+) 495 WP_339080107.1 MOSC domain-containing protein -
  WF513_RS14565 (WF513_14565) - 3066288..3066461 (+) 174 WP_339080108.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43876.55 Da        Isoelectric Point: 10.0658

>NTDB_id=961466 WF513_RS14525 WP_339080100.1 3060570..3061787(+) (pilC) [Pseudomonas sp. TMP9]
MAVKALKTSTFAWEGTNKQGAKIKGEVSGQNPALVKAQLRKQGINPTKVRKKSASLFSAGKKIKPMDIALFARQMATMMK
AGVPLLQSFDIIAEGVDNPNMRKLVDDIKQHVAAGNSFAASLRTRPEYFDDLFCNLVDAGEQAGALENLLDRVATYKEKT
EALKSKIKKAMNYPIAVVLVAVIVSAILLIKVVPQFESVFANFGAELPAFTQFVIGISQGLQKWWFIILVGFFAVGFTFV
ETKKRSEKLRNWLDRTALKAPIVGDIIYKSSVARYARTLATTFAAGVPLVEALDSVAGATGNIVFKNAVNKIKQDVSAGM
QLNFSMRSTAVFPSMAIQMTAIGEESGALDMMLDKVASYYEAEVDNAVDGLTSLMEPLIMSVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=961466 WF513_RS14525 WP_339080100.1 3060570..3061787(+) (pilC) [Pseudomonas sp. TMP9]
ATGGCTGTTAAAGCACTAAAAACCAGCACCTTTGCCTGGGAAGGCACCAACAAGCAGGGTGCCAAAATAAAAGGTGAAGT
CAGCGGCCAGAACCCCGCCTTGGTCAAAGCGCAGCTGCGTAAGCAGGGCATTAACCCAACCAAGGTCCGCAAAAAGTCTG
CCTCGCTGTTCAGCGCGGGGAAAAAGATTAAACCCATGGACATTGCGCTGTTTGCCCGGCAAATGGCCACGATGATGAAG
GCTGGCGTACCGCTGCTGCAGTCCTTTGACATTATTGCCGAGGGTGTGGACAACCCGAACATGCGCAAGCTGGTGGATGA
CATCAAACAGCATGTAGCAGCCGGTAATAGCTTTGCGGCCTCCCTGCGAACTCGACCTGAATATTTTGATGATCTATTTT
GCAACTTGGTCGATGCCGGCGAGCAAGCAGGCGCACTGGAAAACCTACTGGATCGCGTTGCAACCTACAAAGAAAAGACC
GAAGCACTCAAGTCAAAAATCAAAAAAGCGATGAATTACCCCATCGCAGTAGTATTGGTGGCGGTGATTGTGTCGGCTAT
TTTGCTGATCAAAGTGGTGCCGCAATTCGAGTCGGTATTTGCTAACTTTGGCGCTGAACTGCCGGCCTTTACGCAGTTTG
TAATCGGTATTTCTCAAGGGCTTCAAAAATGGTGGTTTATCATTCTGGTTGGTTTCTTCGCTGTAGGTTTTACTTTTGTT
GAGACCAAGAAACGCTCGGAAAAACTCCGTAACTGGCTCGACCGCACGGCACTGAAAGCGCCGATTGTCGGTGACATCAT
CTACAAGTCATCTGTTGCTCGGTATGCCCGCACCCTGGCCACCACCTTCGCCGCCGGTGTGCCGCTGGTAGAAGCGCTGG
ATTCTGTAGCCGGCGCCACTGGTAATATCGTGTTTAAGAATGCTGTGAATAAAATCAAGCAAGATGTCAGCGCCGGTATG
CAGCTGAACTTCTCCATGCGCAGCACGGCAGTATTCCCTTCAATGGCCATCCAGATGACTGCCATTGGTGAAGAGTCCGG
CGCGCTGGACATGATGCTGGACAAAGTCGCCAGCTATTATGAGGCTGAGGTGGATAACGCTGTGGACGGTTTGACCAGCC
TTATGGAACCGCTGATTATGTCAGTGCTCGGCGTGCTGGTCGGCGGCCTGATCATTGCCATGTACCTGCCTATCTTCCAA
CTGGGTGCTGTTGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

60.539

100

0.61
  pilC Acinetobacter baylyi ADP1

60.453

98.025

0.593
  pilC Legionella pneumophila strain ERS1305867

54.156

98.025

0.531
  pilG Neisseria gonorrhoeae MS11

44.417

99.506

0.442
  pilG Neisseria meningitidis 44/76-A

44.417

99.506

0.442
  pilC Vibrio cholerae strain A1552

41.523

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.657

97.778

0.398