Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WF513_RS14520 Genome accession   NZ_CP149803
Coordinates   3058863..3060566 (+) Length   567 a.a.
NCBI ID   WP_339080099.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3053863..3065566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF513_RS14505 (WF513_14505) nadC 3056419..3057282 (+) 864 WP_339080096.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WF513_RS14510 (WF513_14510) tfpZ 3057310..3058083 (-) 774 WP_339080097.1 TfpX/TfpZ family type IV pilin accessory protein -
  WF513_RS14515 (WF513_14515) pilA2 3058137..3058640 (-) 504 WP_339080098.1 pilin Machinery gene
  WF513_RS14520 (WF513_14520) pilB 3058863..3060566 (+) 1704 WP_339080099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WF513_RS14525 (WF513_14525) pilC 3060570..3061787 (+) 1218 WP_339080100.1 type II secretion system F family protein Machinery gene
  WF513_RS14530 (WF513_14530) pilD 3061791..3062660 (+) 870 WP_339080101.1 A24 family peptidase Machinery gene
  WF513_RS14535 (WF513_14535) coaE 3063079..3063693 (+) 615 WP_339080102.1 dephospho-CoA kinase -
  WF513_RS14540 (WF513_14540) yacG 3063690..3063890 (+) 201 WP_339080103.1 DNA gyrase inhibitor YacG -
  WF513_RS14545 (WF513_14545) - 3063952..3064167 (-) 216 WP_339080104.1 hypothetical protein -
  WF513_RS14550 (WF513_14550) - 3064272..3064961 (-) 690 WP_339080105.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62527.59 Da        Isoelectric Point: 5.4843

>NTDB_id=961465 WF513_RS14520 WP_339080099.1 3058863..3060566(+) (pilB) [Pseudomonas sp. TMP9]
MNDSVSLTGLARQMVLAELLDEKTAQQAQAQAQRNKLSLVTYLVQNKLVKSRVLAELASEQFGVSFVDLNAIDKESQPKE
LVTEKLIRQHRVLPLWRRGNKLFVAVSDPTNHQAITDVQFSTGMNTESILVEDDKLGDAIEKFFDSTGSGMEDLADVDLD
GLDVEAVSDDKEVTGDGNSADDAPVVRFVNKMLLDAIKKGSSDLHFEPYEKTYRVRFRTDGILQEIARPPIQLSPRISAR
LKVMAGLDISERRRPQDGRIKMRISKTKSIDFRVSTCPTLWGEKIVMRILDSASAQMGIDALGYEESQKALYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKSFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSSCKKEIQIPREALLEEGFP
EDKIGTFKIYEPVGCENCNGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDISIQMRKDGFNDLRTSALLKAMQGVTSLAE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=961465 WF513_RS14520 WP_339080099.1 3058863..3060566(+) (pilB) [Pseudomonas sp. TMP9]
ATGAACGATAGCGTGTCCCTTACCGGCCTGGCCCGGCAGATGGTGTTAGCAGAACTGCTGGATGAAAAAACCGCGCAACA
GGCGCAAGCCCAAGCGCAGCGCAATAAGCTGTCGCTGGTGACCTACCTGGTGCAGAACAAGCTGGTGAAGAGCCGAGTAT
TGGCCGAACTGGCCAGCGAGCAATTTGGTGTGTCCTTTGTTGACCTGAATGCCATCGACAAAGAAAGCCAGCCAAAAGAG
CTGGTAACCGAAAAGCTGATTCGTCAGCACCGCGTCCTGCCGCTGTGGCGGCGGGGCAACAAGCTGTTTGTCGCGGTATC
CGACCCCACCAACCACCAAGCCATCACCGACGTTCAGTTCAGCACCGGGATGAACACCGAGTCGATTCTGGTCGAGGATG
ACAAACTCGGTGACGCCATCGAGAAATTTTTTGATTCCACCGGCAGCGGTATGGAGGACTTGGCAGACGTTGATTTAGAC
GGCCTAGATGTTGAGGCCGTCAGTGATGACAAGGAAGTTACGGGCGACGGCAACTCCGCCGATGACGCGCCGGTGGTGCG
CTTTGTAAATAAGATGCTGCTCGACGCCATCAAAAAAGGCTCCTCCGACCTGCACTTTGAGCCTTATGAGAAGACCTACC
GGGTGCGCTTTCGCACCGATGGTATTTTGCAAGAAATCGCCCGCCCACCCATCCAGCTCTCGCCACGCATCTCTGCGCGC
TTGAAAGTAATGGCCGGCTTGGATATTTCCGAGCGGCGCAGGCCGCAGGATGGGCGCATCAAGATGCGCATCTCGAAAAC
TAAATCCATCGACTTTCGCGTCAGCACCTGCCCAACGCTGTGGGGCGAAAAGATCGTCATGCGGATTCTCGATTCCGCCA
GCGCACAGATGGGCATTGATGCGCTGGGTTATGAAGAGTCGCAAAAAGCGCTTTACATGGCCGCACTCAAGCAACCGCAG
GGCATGATTTTGGTCACGGGCCCCACCGGCTCCGGCAAAACCGTATCGCTGTACACCGGCTTGAATATTCTCAACACCAC
GGACGTGAATATCTCCACGGCGGAAGACCCTGTGGAGATCAACCTCGAGGGCATTAACCAGGTCAACGTCAACCCCAAGC
AGGGCATGGACTTTACCGCCGCACTGAAATCCTTCCTACGGCAAGACCCGGACATCATCATGGTCGGGGAGATTCGTGAC
CTGGATACCGCCTCTATTGCCATCAAGGCCGCACAAACCGGACACATGGTGATGTCGACCCTGCACACCAACAGCGCCGC
AGAAACCCTGACCCGCCTGCGCAATATGGGCGTACCGTCGTTTAACATCGCCACCTCGGTGAATCTGATCATCGCCCAAC
GTTTGGCGCGCAAGCTGTGCAGCAGCTGCAAGAAGGAAATCCAGATCCCCCGCGAGGCCCTGTTAGAGGAAGGCTTCCCG
GAAGACAAGATCGGCACCTTTAAAATTTACGAGCCGGTGGGCTGCGAAAATTGCAACGGCGGTTACAAAGGCCGTGTCGG
AATTTATGAAGTGGTTAAAAACACGCCTGCCCTGCAACGGATTATCATGGAAGAAGGCAACTCGATTGATATCTCCATCC
AGATGCGCAAAGACGGCTTTAATGACCTGCGAACCTCAGCCTTGCTGAAGGCCATGCAAGGCGTGACCAGCCTCGCTGAA
GTGAACCGAGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.298

100

0.596
  pilB Acinetobacter baylyi ADP1

58.554

100

0.586
  pilB Legionella pneumophila strain ERS1305867

54.867

99.647

0.547
  pilB Vibrio campbellii strain DS40M4

50

99.471

0.497
  pilB Vibrio cholerae strain A1552

50.357

98.765

0.497
  pilF Neisseria gonorrhoeae MS11

49.734

99.295

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

51.013

95.767

0.489
  pilF Thermus thermophilus HB27

36.46

99.647

0.363