Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   WF513_RS14515 Genome accession   NZ_CP149803
Coordinates   3058137..3058640 (-) Length   167 a.a.
NCBI ID   WP_339080098.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3053137..3063640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WF513_RS14500 (WF513_14500) - 3053738..3056053 (-) 2316 WP_339080095.1 DUF1631 domain-containing protein -
  WF513_RS14505 (WF513_14505) nadC 3056419..3057282 (+) 864 WP_339080096.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WF513_RS14510 (WF513_14510) tfpZ 3057310..3058083 (-) 774 WP_339080097.1 TfpX/TfpZ family type IV pilin accessory protein -
  WF513_RS14515 (WF513_14515) pilA2 3058137..3058640 (-) 504 WP_339080098.1 pilin Machinery gene
  WF513_RS14520 (WF513_14520) pilB 3058863..3060566 (+) 1704 WP_339080099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WF513_RS14525 (WF513_14525) pilC 3060570..3061787 (+) 1218 WP_339080100.1 type II secretion system F family protein Machinery gene
  WF513_RS14530 (WF513_14530) pilD 3061791..3062660 (+) 870 WP_339080101.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 167 a.a.        Molecular weight: 16542.10 Da        Isoelectric Point: 8.4830

>NTDB_id=961464 WF513_RS14515 WP_339080098.1 3058137..3058640(-) (pilA2) [Pseudomonas sp. TMP9]
MKAQMQKGFTLIELMIVVAIIGILAAVALPAYQDYTTRARVTEGLNLASSAKINVADVLASGNPGADALGYNIGYTSPAA
TRNTTSVNITAATGVITITTTAAAGGGTIIMTPNAPVGTILPVGTAAFTPPAEAVSWRCMAAGAAAAGFGGTAAGTLPAR
FAPAECK

Nucleotide


Download         Length: 504 bp        

>NTDB_id=961464 WF513_RS14515 WP_339080098.1 3058137..3058640(-) (pilA2) [Pseudomonas sp. TMP9]
ATGAAAGCTCAAATGCAGAAAGGCTTTACCCTGATTGAACTGATGATTGTTGTAGCGATTATCGGTATTTTGGCTGCTGT
TGCACTTCCGGCTTATCAGGATTACACCACTCGCGCCCGTGTAACTGAGGGTTTGAACCTGGCGTCATCCGCCAAGATTA
ACGTTGCAGACGTTTTGGCTTCTGGCAATCCCGGTGCTGATGCTCTTGGTTATAACATCGGCTACACCAGCCCTGCTGCC
ACACGTAACACGACTAGCGTCAATATCACTGCGGCTACTGGCGTGATTACGATAACAACAACTGCTGCTGCTGGTGGCGG
CACTATTATCATGACCCCGAACGCTCCTGTAGGTACTATTCTGCCTGTAGGTACCGCAGCATTTACGCCACCAGCTGAAG
CTGTTTCCTGGCGCTGCATGGCTGCTGGTGCTGCCGCCGCTGGTTTTGGTGGTACCGCTGCAGGTACTTTGCCAGCGCGT
TTTGCTCCTGCAGAATGCAAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-167)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

53.659

98.204

0.527
  pilA2 Legionella pneumophila str. Paris

53.049

98.204

0.521
  pilA Ralstonia pseudosolanacearum GMI1000

47.093

100

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.816

100

0.479
  comP Acinetobacter baylyi ADP1

38.372

100

0.395
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.059

100

0.377