Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WJM78_RS18600 Genome accession   NZ_CP149129
Coordinates   4026840..4028081 (+) Length   413 a.a.
NCBI ID   WP_339010930.1    Uniprot ID   -
Organism   Aeromonas popoffii strain BE5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4021840..4033081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS18575 ampD 4022195..4022767 (-) 573 WP_339015921.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WJM78_RS18580 - 4022907..4023377 (+) 471 WP_181364745.1 retropepsin-like aspartic protease -
  WJM78_RS18585 nadC 4023383..4024240 (+) 858 WP_042039021.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WJM78_RS18590 - 4024560..4024997 (+) 438 WP_339010926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WJM78_RS18595 pilB 4025001..4026707 (+) 1707 WP_339010928.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WJM78_RS18600 pilC 4026840..4028081 (+) 1242 WP_339010930.1 type II secretion system F family protein Machinery gene
  WJM78_RS18605 pilD 4028146..4029018 (+) 873 WP_339010932.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  WJM78_RS18610 coaE 4029036..4029650 (+) 615 WP_339010934.1 dephospho-CoA kinase -
  WJM78_RS18615 zapD 4029688..4030410 (+) 723 WP_339010936.1 cell division protein ZapD -
  WJM78_RS18620 yacG 4030420..4030614 (+) 195 WP_042039030.1 DNA gyrase inhibitor YacG -
  WJM78_RS18625 mutT 4030687..4031094 (-) 408 WP_181364743.1 8-oxo-dGTP diphosphatase MutT -
  WJM78_RS18630 - 4031103..4032287 (-) 1185 WP_339010938.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45288.44 Da        Isoelectric Point: 9.8081

>NTDB_id=956747 WJM78_RS18600 WP_339010930.1 4026840..4028081(+) (pilC) [Aeromonas popoffii strain BE5]
MATLTQKQNAPKKVFSFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIRPMDIAIVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPLHFDALYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQVVIGISRFMQHWWFVIFGGTA
LAIFLYVRAWRTSQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGDVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=956747 WJM78_RS18600 WP_339010930.1 4026840..4028081(+) (pilC) [Aeromonas popoffii strain BE5]
ATGGCAACGCTAACGCAGAAACAGAATGCCCCCAAGAAAGTCTTCTCCTTCCGCTGGAGCGGAGTGAACCGCAAGGGTCA
GAAGGTCTCCGGTGAGCTGCAGGCCGACAGTATCAACACGGTCAAAGCTGAGCTGCGCAAGCAGGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGATTATTCTCCAAGGGTGGTGCCAAGATCAGGCCGATGGATATTGCCATCGTCTCCCGT
CAAATCACTACCATGCTTTCCGCTGGCGTCCCCTTGGTACAGAGCCTGCAGATAATCGCCCGCAGCCACGAAAAAGCCGC
GATGCGTGAACTCATGGGACAGATCGCCGCCGACGTGGAAACCGGCACCCCCATGTCCGAGGCGCTGCGCCGCCATCCCC
TCCACTTTGATGCGCTCTATTGTGATCTGGTGGAGGCAGGGGAACAATCCGGTGCGCTGGAGACTATCTATGACCGGATC
GCCACCTATCGGGAAAAGTCGGAGGCGCTCAAGTCCAAGATCAAGAAGGCGATGTTTTACCCAGCCATGGTCATACTGGT
TGCCATCGTTGTCACCTCCATACTGCTGCTGTTCGTCATTCCGCAGTTTGAGGATATTTTCAAGAGCTTCGGTGCCGAAC
TGCCCATCTTCACCCAGGTTGTCATCGGCATCTCTCGCTTCATGCAGCATTGGTGGTTTGTCATCTTTGGCGGCACTGCA
CTCGCCATTTTCCTCTATGTACGGGCATGGCGCACCTCCCAGAAGGTCAAAGACAACACCGACAAGTTCATCCTCACCAT
TCCTGTGGTCGGCATGATACTGCACAAGGCGGCCATGGCCCGTTTCGCCCGAACCCTCTCCACCACCTTCTCCGCCGGGA
TCCCGCTGGTGGATGCACTGATCTCGGCCGCAGGTGCCTCCGGCAACTATGTCTATCGCACCGCCGTCATGGCCATTCGC
AACGAGGTGGTCGCCGGCATGCAGATCAACGTGGCCATGCGTACTGTGGATCTCTTCCCCGACATGGTGATCCAAATGGT
GATGATCGGCGAGGAGTCCGGTGCCATCGATGATATGCTCTCCAAGGTTGCAACCATCTTTGAGCAGGAGGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTCGAGCCCCTCATCATGGTAGTGCTGGGGGTGCTGGTTGGCGGCATGGTTGTGGCC
ATGTACCTGCCCATCTTCAAGCTGGGTGACGTAGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

95.884

0.542
  pilC Acinetobacter baumannii D1279779

54.364

97.094

0.528
  pilC Acinetobacter baylyi ADP1

52.393

96.126

0.504
  pilC Legionella pneumophila strain ERS1305867

51.111

98.063

0.501
  pilC Vibrio cholerae strain A1552

47.304

98.789

0.467
  pilC Vibrio campbellii strain DS40M4

46.366

96.61

0.448
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

38.06

97.337

0.37