Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WJM78_RS18605 Genome accession   NZ_CP149129
Coordinates   4028146..4029018 (+) Length   290 a.a.
NCBI ID   WP_339010932.1    Uniprot ID   -
Organism   Aeromonas popoffii strain BE5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4023146..4034018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS18585 nadC 4023383..4024240 (+) 858 WP_042039021.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WJM78_RS18590 - 4024560..4024997 (+) 438 WP_339010926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WJM78_RS18595 pilB 4025001..4026707 (+) 1707 WP_339010928.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WJM78_RS18600 pilC 4026840..4028081 (+) 1242 WP_339010930.1 type II secretion system F family protein Machinery gene
  WJM78_RS18605 pilD 4028146..4029018 (+) 873 WP_339010932.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  WJM78_RS18610 coaE 4029036..4029650 (+) 615 WP_339010934.1 dephospho-CoA kinase -
  WJM78_RS18615 zapD 4029688..4030410 (+) 723 WP_339010936.1 cell division protein ZapD -
  WJM78_RS18620 yacG 4030420..4030614 (+) 195 WP_042039030.1 DNA gyrase inhibitor YacG -
  WJM78_RS18625 mutT 4030687..4031094 (-) 408 WP_181364743.1 8-oxo-dGTP diphosphatase MutT -
  WJM78_RS18630 - 4031103..4032287 (-) 1185 WP_339010938.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32293.49 Da        Isoelectric Point: 6.6818

>NTDB_id=956748 WJM78_RS18605 WP_339010932.1 4028146..4029018(+) (pilD) [Aeromonas popoffii strain BE5]
MTLLLELAHNLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYHSYFTPDDAGGDEPPYNLMGPRSCCPHCHHP
ITALENIPLLSWLWLKGRCRGCQAPISARYPLVELLTALLSVAVAMTVPPGWGTLAALLLTWVLVALTFIDFDKMLLPDQ
LTLPLLWGGLLFNLLGGFVSLGDAVIGAMTGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
IGAFMGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=956748 WJM78_RS18605 WP_339010932.1 4028146..4029018(+) (pilD) [Aeromonas popoffii strain BE5]
ATGACACTTCTCCTCGAGCTGGCACACAACTTGCCCTGGCTCTATTTCTCGCTGGTATTTCTCTTTTCCCTGATGATCGG
CAGTTTTCTCAACGTGGTGATCCATCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCACAGCTACTTCA
CCCCTGATGACGCGGGGGGCGACGAGCCGCCCTACAACCTGATGGGGCCACGCTCCTGCTGCCCTCACTGCCATCACCCC
ATCACGGCACTGGAAAACATCCCCTTGCTGAGCTGGCTCTGGCTCAAGGGTCGCTGCCGAGGCTGCCAGGCTCCCATATC
GGCCCGCTATCCGCTGGTCGAGCTGCTGACCGCCCTGCTCTCGGTTGCCGTTGCCATGACGGTTCCCCCCGGCTGGGGCA
CGCTGGCAGCCCTGCTACTGACCTGGGTGCTGGTGGCTCTCACCTTTATCGATTTCGACAAGATGCTGCTGCCGGACCAG
CTCACCTTGCCCCTGCTGTGGGGCGGGCTGCTGTTCAACTTACTGGGCGGTTTTGTATCGCTTGGCGATGCGGTGATCGG
CGCCATGACCGGTTATCTGGTGCTATGGAGCCTCTATTGGGCATTCAAGCTGCTCACTGGCAAGGAGGGCATGGGCTATG
GTGACTTCAAGCTGCTGGCCGCGCTGGGTGCCTGGCTGGGCTGGCAAGCGCTGCCCATAGTGCTGCTGCTCTCCTCCCTG
ATCGGTGCCTTCATGGGCATAGGCCTGATCCTGTTGCGCAACCACCATCAGAGTAAACCCATTCCCTTTGGCCCCTATCT
CGCTATCGCGGGCTGGATTGCGCTGCTGTGGGGCGATAGCATCACCCGCTGGTATCTCACGACTTTCCTCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

61.324

98.966

0.607
  pilD Vibrio campbellii strain DS40M4

53.659

98.966

0.531
  pilD Acinetobacter nosocomialis M2

55.036

95.862

0.528
  pilD Acinetobacter baumannii D1279779

54.317

95.862

0.521
  pilD Neisseria gonorrhoeae MS11

49.817

94.138

0.469