Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WJM78_RS18595 Genome accession   NZ_CP149129
Coordinates   4025001..4026707 (+) Length   568 a.a.
NCBI ID   WP_339010928.1    Uniprot ID   -
Organism   Aeromonas popoffii strain BE5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4020001..4031707
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS18570 pdhR 4021154..4021918 (-) 765 WP_042039018.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WJM78_RS18575 ampD 4022195..4022767 (-) 573 WP_339015921.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WJM78_RS18580 - 4022907..4023377 (+) 471 WP_181364745.1 retropepsin-like aspartic protease -
  WJM78_RS18585 nadC 4023383..4024240 (+) 858 WP_042039021.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WJM78_RS18590 - 4024560..4024997 (+) 438 WP_339010926.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WJM78_RS18595 pilB 4025001..4026707 (+) 1707 WP_339010928.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WJM78_RS18600 pilC 4026840..4028081 (+) 1242 WP_339010930.1 type II secretion system F family protein Machinery gene
  WJM78_RS18605 pilD 4028146..4029018 (+) 873 WP_339010932.1 type IVa prepilin peptidase/methyltransferase TapD Machinery gene
  WJM78_RS18610 coaE 4029036..4029650 (+) 615 WP_339010934.1 dephospho-CoA kinase -
  WJM78_RS18615 zapD 4029688..4030410 (+) 723 WP_339010936.1 cell division protein ZapD -
  WJM78_RS18620 yacG 4030420..4030614 (+) 195 WP_042039030.1 DNA gyrase inhibitor YacG -
  WJM78_RS18625 mutT 4030687..4031094 (-) 408 WP_181364743.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62558.94 Da        Isoelectric Point: 5.3147

>NTDB_id=956746 WJM78_RS18595 WP_339010928.1 4025001..4026707(+) (pilB) [Aeromonas popoffii strain BE5]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIESEILDSKTLADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQEALGMEDIDESEI
SELEVSDENSRLDESVNAADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKTPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKDCSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=956746 WJM78_RS18595 WP_339010928.1 4025001..4026707(+) (pilB) [Aeromonas popoffii strain BE5]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGATAAGTGAAGAGGACTCCCAGCGCTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTTGTGACCTTTCTGATAGAGAGCGAGATCCTCGACAGCAAGACGCTGG
CGGATTTCTGTGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATACCGCAGAAATAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCATACCCTCTATATCGCCATGTCGGA
TCCGACTAATGTATCGGCGCTGGAGGATTTTGGCTTCAGCTTCGGGCTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AGCTGACGGCCGCCATCGGCAAACTGCTGGAGAGCGAGCAGGAAGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGGCTGGATGAGAGCGTCAATGCCGCTGACGACGATGCCCCCATCGTCAA
ATACATTAACAAAATCATGATGGATGCCATCAAGCGTGGCGCATCCGATCTGCACTTCGAACCCTATGAGACCAAATACC
GCATCCGCTTCCGGATCGACGGCATCTTACACGAAATCGCGACGCCACCGGTTAATCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTCATGGCACGGCTCGACATTGCCGAGCGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCTGTCCCGCAA
CAAGTCGATGGATATGCGGGTTAACACCCTGCCCACCATGTGGGGTGAGAAGATCGTCATCCGGCTGCTGGACTCCTCGG
CGGCGCGGCTCAACATAGAGCAGCTCGGCTTTGACGACCGGCAGAAAGCACAATATCTGCACGCCCTTTCCAAACCGCAG
GGCATGATATTGGTCACAGGCCCCACCGGCTCGGGCAAAACTGTCTCCCTCTACACCGGTCTTAATATTCTCAACACCAC
AGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAATCTGCCCGGCGTCAATCAGGTGCAAGTCAACCCAAAGG
CAGGGCTGACCTTCGCCAGCGCCCTGCGCTCGTTTCTTCGCCAGGATCCCGACGTGGTCATGGTGGGGGAGATCCGGGAT
CTGGAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACAGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCCGC
CGAGACACTAACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATTGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GACTCGCCCGCAAGCTGTGCGATCACTGCAAGACACCCGAAGTAGTACCAGAAGCCGAGCTGCTGGAGCTGGGATTCACC
CAGCAGCAGCTGGCCGCCGGGCTGCGCCTGTTCAAACCCGTGGGCTGCAAGGATTGCTCGGGCGGTTACAAGGGGCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGTGCCAACTCGCTGCAAATTGCAG
CCATTGCCCAAAAAGAGGGGATGCGCACTCTGCGTGTATCCGGCCTTGAGAAAGCTCGTCTGGGAGTGACCAGCCTGGCA
GAAATCAATCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.282

100

0.553
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Legionella pneumophila strain ERS1305867

55.801

95.599

0.533
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

51.068

98.944

0.505
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375