Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   WE348_RS14575 Genome accession   NZ_CP147978
Coordinates   3372967..3373392 (+) Length   141 a.a.
NCBI ID   WP_338852060.1    Uniprot ID   -
Organism   Alteromonas macleodii strain KD01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3367967..3378392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WE348_RS14550 (WE348_14550) pdhR 3368212..3368958 (-) 747 WP_014950397.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WE348_RS14555 (WE348_14555) ampE 3369324..3370226 (-) 903 WP_338852058.1 beta-lactamase regulator AmpE -
  WE348_RS14560 (WE348_14560) ampD 3370269..3370808 (-) 540 WP_069944747.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WE348_RS14565 (WE348_14565) - 3370992..3371510 (+) 519 WP_014977216.1 TIGR02281 family clan AA aspartic protease -
  WE348_RS14570 (WE348_14570) nadC 3371543..3372397 (+) 855 WP_131138172.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WE348_RS14575 (WE348_14575) pilA2 3372967..3373392 (+) 426 WP_338852060.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  WE348_RS14580 (WE348_14580) pilC 3373483..3374691 (+) 1209 WP_338852062.1 type II secretion system F family protein Machinery gene
  WE348_RS14585 (WE348_14585) pilD 3374744..3375637 (+) 894 WP_338852064.1 A24 family peptidase Machinery gene
  WE348_RS14590 (WE348_14590) coaE 3375656..3376267 (+) 612 WP_283625637.1 dephospho-CoA kinase -
  WE348_RS14595 (WE348_14595) zapD 3376511..3377263 (+) 753 WP_039227984.1 cell division protein ZapD -
  WE348_RS14600 (WE348_14600) yacG 3377397..3377624 (+) 228 WP_014950407.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14207.23 Da        Isoelectric Point: 4.1672

>NTDB_id=949811 WE348_RS14575 WP_338852060.1 3372967..3373392(+) (pilA2) [Alteromonas macleodii strain KD01]
MMNMNTKNQKGFTLIELMIVVAIIGILAAIALPAYQTYTARATYSEVISASSAAKTAVEVCAQTGVPADCSTITVNPGWT
NAPTVDTLTLGGDAANGYTITVTPNAGSGIAATDTLILTGTLNNGAVTWATTGGCVTSGLC

Nucleotide


Download         Length: 426 bp        

>NTDB_id=949811 WE348_RS14575 WP_338852060.1 3372967..3373392(+) (pilA2) [Alteromonas macleodii strain KD01]
ATGATGAACATGAATACTAAAAACCAAAAAGGTTTCACCCTAATCGAACTAATGATTGTTGTTGCAATCATCGGTATCCT
TGCAGCAATCGCACTTCCTGCCTATCAGACCTATACTGCACGTGCAACGTACAGCGAAGTAATTTCTGCTTCTAGCGCTG
CTAAAACTGCGGTTGAAGTATGTGCTCAAACGGGTGTTCCAGCTGACTGCAGCACTATTACTGTAAACCCAGGTTGGACG
AATGCACCAACAGTTGATACTTTAACATTAGGTGGCGATGCAGCTAACGGCTACACCATTACAGTAACTCCAAATGCAGG
TAGTGGGATAGCTGCTACGGACACTCTTATTCTAACAGGCACTCTAAATAATGGTGCGGTTACATGGGCGACAACTGGCG
GTTGCGTAACATCTGGCCTTTGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-132)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

53.03

93.617

0.496
  pilA2 Legionella pneumophila str. Paris

52.273

93.617

0.489
  comP Acinetobacter baylyi ADP1

44.776

95.035

0.426
  pilA Vibrio campbellii strain DS40M4

38.776

100

0.404
  pilA Haemophilus influenzae Rd KW20

41.667

93.617

0.39
  pilA Pseudomonas aeruginosa PAK

41.6

88.652

0.369
  pilA Vibrio parahaemolyticus RIMD 2210633

38.346

94.326

0.362
  pilA Haemophilus influenzae 86-028NP

41.129

87.943

0.362
  comF Acinetobacter baylyi ADP1

41.463

87.234

0.362