Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   WDW95_RS10485 Genome accession   NZ_CP147857
Coordinates   2096276..2097217 (-) Length   313 a.a.
NCBI ID   WP_061774317.1    Uniprot ID   -
Organism   Lactococcus raffinolactis strain APC3967     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2091276..2102217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDW95_RS10440 - 2091294..2091743 (-) 450 WP_096039675.1 zinc-dependent MarR family transcriptional regulator -
  WDW95_RS10445 - 2091819..2092667 (-) 849 WP_167838309.1 aminotransferase class IV -
  WDW95_RS10450 ispE 2092751..2093602 (-) 852 WP_061774322.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
  WDW95_RS10455 comGG 2093607..2093891 (-) 285 WP_368651551.1 competence type IV pilus minor pilin ComGG -
  WDW95_RS10460 comGF 2093903..2094346 (-) 444 WP_244165991.1 competence type IV pilus minor pilin ComGF -
  WDW95_RS10465 comGE 2094321..2094626 (-) 306 WP_082785376.1 competence type IV pilus minor pilin ComGE -
  WDW95_RS10470 comGD 2094598..2095083 (-) 486 WP_368651552.1 competence type IV pilus minor pilin ComGD -
  WDW95_RS10475 comYC 2094998..2095318 (-) 321 WP_061774318.1 competence type IV pilus major pilin ComGC Machinery gene
  WDW95_RS10480 comYB 2095327..2096394 (-) 1068 WP_368651553.1 competence type IV pilus assembly protein ComGB Machinery gene
  WDW95_RS10485 comGA/cglA/cilD 2096276..2097217 (-) 942 WP_061774317.1 competence type IV pilus ATPase ComGA Machinery gene
  WDW95_RS10490 - 2097280..2099124 (-) 1845 WP_096039681.1 acyltransferase family protein -
  WDW95_RS10495 - 2099121..2099483 (-) 363 WP_061774315.1 hypothetical protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 34933.21 Da        Isoelectric Point: 9.1747

>NTDB_id=949016 WDW95_RS10485 WP_061774317.1 2096276..2097217(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain APC3967]
MIKEIAQRMLKKASDFGASDIYILPARTGFSVVFRKSAHREYDQLLSDAEGQSLISHFKFTAGMNVGEKRRPQLGSCLYE
LADRKCRLRLSSAGDFESRESLVIRILHDTKQPLKFWIEADLPQVKKLVARRGLYLFAGPVGSGKTTLMHHIAKEKFSGQ
QVITIEDPVEIVAPDLLQFQLNEAIGNTYDSLIKLSLRHMPDLVIVGEIRDQETARAVMRASLTGYTVFSTIHAKSIAGV
YARLLELGVTKEEINNSLSGVVYQRLIAGKGVLDSAEKAFERHANDKWHAKIEKLVTEGHLTPDQATAEKVSD

Nucleotide


Download         Length: 942 bp        

>NTDB_id=949016 WDW95_RS10485 WP_061774317.1 2096276..2097217(-) (comGA/cglA/cilD) [Lactococcus raffinolactis strain APC3967]
ATGATAAAGGAAATAGCGCAAAGGATGTTAAAGAAAGCAAGCGATTTTGGGGCAAGTGATATTTATATATTACCCGCCAG
AACAGGATTTTCGGTTGTTTTCAGAAAGTCAGCACATCGTGAATATGATCAGCTCCTGTCAGATGCTGAAGGTCAAAGTC
TCATTTCCCATTTCAAATTTACAGCAGGGATGAATGTAGGTGAGAAGCGCCGGCCACAACTAGGATCTTGCTTGTATGAG
CTTGCAGATAGAAAGTGTCGCTTGCGTCTATCCTCAGCGGGTGACTTTGAAAGTCGCGAAAGTTTGGTGATTCGAATTTT
GCATGACACGAAACAACCACTCAAGTTTTGGATTGAGGCAGATTTACCTCAGGTAAAAAAGTTAGTAGCTAGACGCGGTC
TTTATTTATTTGCGGGGCCAGTTGGTTCTGGGAAAACGACGCTGATGCATCACATTGCCAAAGAAAAGTTTTCTGGTCAG
CAAGTCATTACGATTGAAGATCCAGTTGAAATCGTTGCACCAGATCTGCTCCAATTCCAACTGAATGAAGCGATTGGCAA
CACCTATGATAGCTTGATTAAATTATCCTTGCGCCATATGCCAGATCTTGTGATTGTCGGAGAAATTCGAGATCAGGAAA
CTGCGCGAGCAGTCATGAGGGCCAGTCTGACGGGCTATACTGTTTTTTCGACGATTCATGCTAAGTCGATTGCGGGTGTT
TATGCGCGATTATTGGAGCTTGGCGTAACAAAAGAAGAAATCAATAATTCGTTGTCAGGTGTTGTCTATCAACGGTTGAT
TGCAGGAAAGGGGGTGCTAGATAGTGCTGAAAAAGCATTCGAAAGGCATGCCAACGACAAGTGGCATGCCAAAATTGAAA
AATTGGTTACAGAAGGACATCTCACACCTGATCAGGCGACAGCCGAAAAGGTTAGCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

57.692

99.681

0.575
  comGA/cglA/cilD Streptococcus pneumoniae R6

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae D39

56.731

99.681

0.565
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

56.731

99.681

0.565
  comGA Lactococcus lactis subsp. cremoris KW2

56.23

100

0.562
  comYA Streptococcus gordonii str. Challis substr. CH1

55.911

100

0.559
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

53.674

100

0.537
  comYA Streptococcus mutans UA140

52.548

100

0.527
  comYA Streptococcus mutans UA159

52.548

100

0.527