Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   V7A35_RS00660 Genome accession   NZ_CP146754
Coordinates   136827..137771 (+) Length   314 a.a.
NCBI ID   WP_040086850.1    Uniprot ID   -
Organism   Lactococcus petauri strain L1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 131827..142771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7A35_RS00645 (V7A35_00645) - 133088..134689 (-) 1602 WP_040086853.1 GH25 family lysozyme -
  V7A35_RS00650 (V7A35_00650) guaB 134858..136339 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  V7A35_RS00655 (V7A35_00655) rpsU 136465..136641 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  V7A35_RS00660 (V7A35_00660) comGA 136827..137771 (+) 945 WP_040086850.1 competence type IV pilus ATPase ComGA Machinery gene
  V7A35_RS00665 (V7A35_00665) comGB 137710..138729 (+) 1020 WP_256777882.1 competence type IV pilus assembly protein ComGB Machinery gene
  V7A35_RS00670 (V7A35_00670) comYC 138748..139053 (+) 306 WP_040087209.1 competence type IV pilus major pilin ComGC Machinery gene
  V7A35_RS00675 (V7A35_00675) comGD 139028..139444 (+) 417 WP_270517134.1 competence type IV pilus minor pilin ComGD Machinery gene
  V7A35_RS00680 (V7A35_00680) comGE 139476..139709 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  V7A35_RS00685 (V7A35_00685) comGF 139696..140130 (+) 435 WP_230625259.1 competence type IV pilus minor pilin ComGF -
  V7A35_RS00690 (V7A35_00690) - 140362..141207 (+) 846 WP_270315874.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  V7A35_RS00695 (V7A35_00695) - 141278..142162 (-) 885 WP_040086846.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35767.15 Da        Isoelectric Point: 7.2081

>NTDB_id=944166 V7A35_RS00660 WP_040086850.1 136827..137771(+) (comGA) [Lactococcus petauri strain L1]
MIQVLAKNLLQKAVDQMVHDLYLVALEGKYSLYFRTATERRFERELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAIAAAREKIEFSKAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=944166 V7A35_RS00660 WP_040086850.1 136827..137771(+) (comGA) [Lactococcus petauri strain L1]
ATGATACAGGTTTTGGCAAAAAACCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAATCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA
TGCCTTGATTAAGCTTTCGCTTCGCCACAGACCTGATTTACTTATTGTTGGTGAAATTAGAGATAGTCAAACGGCAAAAG
CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATCGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTATTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

60.51

100

0.605
  comYA Streptococcus gordonii str. Challis substr. CH1

53.333

100

0.535
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

52.716

99.682

0.525
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.564

99.363

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.396

99.682

0.522
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.396

99.682

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.396

99.682

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

50.949

100

0.513
  comYA Streptococcus mutans UA140

50.949

100

0.513