Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   V7A35_RS00665 Genome accession   NZ_CP146754
Coordinates   137710..138729 (+) Length   339 a.a.
NCBI ID   WP_256777882.1    Uniprot ID   -
Organism   Lactococcus petauri strain L1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 132710..143729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V7A35_RS00645 (V7A35_00645) - 133088..134689 (-) 1602 WP_040086853.1 GH25 family lysozyme -
  V7A35_RS00650 (V7A35_00650) guaB 134858..136339 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  V7A35_RS00655 (V7A35_00655) rpsU 136465..136641 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  V7A35_RS00660 (V7A35_00660) comGA 136827..137771 (+) 945 WP_040086850.1 competence type IV pilus ATPase ComGA Machinery gene
  V7A35_RS00665 (V7A35_00665) comGB 137710..138729 (+) 1020 WP_256777882.1 competence type IV pilus assembly protein ComGB Machinery gene
  V7A35_RS00670 (V7A35_00670) comYC 138748..139053 (+) 306 WP_040087209.1 competence type IV pilus major pilin ComGC Machinery gene
  V7A35_RS00675 (V7A35_00675) comGD 139028..139444 (+) 417 WP_270517134.1 competence type IV pilus minor pilin ComGD Machinery gene
  V7A35_RS00680 (V7A35_00680) comGE 139476..139709 (+) 234 WP_019293009.1 competence type IV pilus minor pilin ComGE -
  V7A35_RS00685 (V7A35_00685) comGF 139696..140130 (+) 435 WP_230625259.1 competence type IV pilus minor pilin ComGF -
  V7A35_RS00690 (V7A35_00690) - 140362..141207 (+) 846 WP_270315874.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  V7A35_RS00695 (V7A35_00695) - 141278..142162 (-) 885 WP_040086846.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39510.19 Da        Isoelectric Point: 9.5027

>NTDB_id=944167 V7A35_RS00665 WP_256777882.1 137710..138729(+) (comGB) [Lactococcus petauri strain L1]
MDLSQLLQLRAKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGKSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRKKLVQVATYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTL
FLGWLFSLFVFFYLCRYYWKKKTALEALRLMVKIPFVGRFVQLYLTAYFAREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDSGQKFEDALRFYPIFTKELSLIVEYGELKSKLGKELMIFSEENWSLFFERMERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=944167 V7A35_RS00665 WP_256777882.1 137710..138729(+) (comGB) [Lactococcus petauri strain L1]
ATGGATTTATCTCAGCTATTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAAGCTCATTCAACTCATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTCTCTGAAATTATTGATTTCCTTGAACGATCGGGTCTTACAGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTTAATGGGAAAAGTTTCTCTGGTATTTTGGCGAAGCTAAATTTTTATGAGGATGTT
GTGACCCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTATACTTTAGGATTGATTGAAGAAAAATTGATACGTGT
TTTGAACATCCGCAAAAAGCTTGTCCAAGTTGCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATTATGCTTG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCCTC
TTTTTAGGGTGGCTCTTCAGTTTGTTCGTTTTCTTTTATCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GCTGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTGCAAGAGAATGGG
GAAACTTAATTGCTCAAGCTGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATTTTCAAAGAA
TTCGGTTTTGAGCTTCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCTCTTCGATTTTACCCCATATTTACCAA
GGAACTCTCATTAATTGTGGAATATGGTGAACTAAAGTCCAAGCTAGGGAAAGAGTTAATGATTTTTTCTGAAGAAAACT
GGTCGCTCTTTTTTGAAAGGATGGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCGGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.976

99.115

0.525
  comGB/cglB Streptococcus mitis NCTC 12261

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae D39

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae R6

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae TIGR4

52.537

98.82

0.519
  comGB/cglB Streptococcus mitis SK321

52.537

98.82

0.519
  comGB/cglB Streptococcus pneumoniae Rx1

52.537

98.82

0.519
  comYB Streptococcus gordonii str. Challis substr. CH1

52.395

98.525

0.516
  comYB Streptococcus mutans UA140

52.548

92.625

0.487
  comYB Streptococcus mutans UA159

52.229

92.625

0.484