Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   V8H05_RS05960 Genome accession   NZ_CP146213
Coordinates   1195024..1195452 (+) Length   142 a.a.
NCBI ID   WP_000934793.1    Uniprot ID   A0A6B3IY96
Organism   Staphylococcus aureus strain KWT 2019-23549     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1187760..1231236 1195024..1195452 within 0


Gene organization within MGE regions


Location: 1187760..1231236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8H05_RS05890 (V8H05_05890) - 1187760..1189145 (-) 1386 WP_000861313.1 recombinase family protein -
  V8H05_RS05895 (V8H05_05895) - 1189352..1190032 (-) 681 WP_000392109.1 type II toxin-antitoxin system PemK/MazF family toxin -
  V8H05_RS05900 (V8H05_05900) - 1190148..1190693 (-) 546 WP_000391577.1 Ltp family lipoprotein -
  V8H05_RS05905 (V8H05_05905) - 1190744..1191376 (-) 633 WP_000901358.1 XRE family transcriptional regulator -
  V8H05_RS05910 (V8H05_05910) - 1191530..1191757 (+) 228 WP_000192117.1 helix-turn-helix transcriptional regulator -
  V8H05_RS05915 (V8H05_05915) - 1191780..1192553 (+) 774 WP_001148549.1 phage antirepressor KilAC domain-containing protein -
  V8H05_RS05920 (V8H05_05920) - 1192554..1192778 (+) 225 WP_000187184.1 hypothetical protein -
  V8H05_RS05925 (V8H05_05925) - 1192818..1192994 (+) 177 WP_000993179.1 hypothetical protein -
  V8H05_RS05930 (V8H05_05930) - 1192969..1193199 (-) 231 WP_000395455.1 hypothetical protein -
  V8H05_RS05935 (V8H05_05935) - 1193258..1193395 (+) 138 WP_001568566.1 hypothetical protein -
  V8H05_RS05940 (V8H05_05940) - 1193379..1193540 (+) 162 WP_000066021.1 DUF1270 family protein -
  V8H05_RS05945 (V8H05_05945) - 1193632..1193892 (+) 261 WP_000291092.1 DUF1108 family protein -
  V8H05_RS05950 (V8H05_05950) - 1193907..1194386 (+) 480 WP_000002510.1 siphovirus Gp157 family protein -
  V8H05_RS05955 (V8H05_05955) - 1194386..1195024 (+) 639 WP_001043064.1 ERF family protein -
  V8H05_RS05960 (V8H05_05960) ssbA 1195024..1195452 (+) 429 WP_000934793.1 single-stranded DNA-binding protein Machinery gene
  V8H05_RS05965 (V8H05_05965) - 1195466..1196140 (+) 675 WP_338616734.1 putative HNHc nuclease -
  V8H05_RS05970 (V8H05_05970) - 1196133..1196888 (+) 756 WP_001037657.1 DnaD domain protein -
  V8H05_RS05975 (V8H05_05975) - 1196888..1197244 (+) 357 WP_001132243.1 hypothetical protein -
  V8H05_RS05980 (V8H05_05980) - 1197241..1198482 (+) 1242 WP_001106167.1 DnaB helicase C-terminal domain-containing protein -
  V8H05_RS05985 (V8H05_05985) - 1198479..1198694 (+) 216 WP_001095146.1 hypothetical protein -
  V8H05_RS05990 (V8H05_05990) - 1198697..1198918 (+) 222 WP_001123688.1 DUF3269 family protein -
  V8H05_RS05995 (V8H05_05995) - 1198928..1199353 (+) 426 WP_000451861.1 phage N-6-adenine-methyltransferase -
  V8H05_RS06000 (V8H05_06000) - 1199350..1199754 (+) 405 WP_000049779.1 DUF1064 domain-containing protein -
  V8H05_RS06005 (V8H05_06005) - 1199759..1199944 (+) 186 WP_001187237.1 DUF3113 family protein -
  V8H05_RS06010 (V8H05_06010) - 1199945..1200304 (+) 360 Protein_1178 SA1788 family PVL leukocidin-associated protein -
  V8H05_RS06015 (V8H05_06015) - 1200304..1200558 (+) 255 WP_000022735.1 DUF3310 domain-containing protein -
  V8H05_RS06020 (V8H05_06020) - 1200558..1200806 (+) 249 WP_012068339.1 SAV1978 family virulence-associated passenger protein -
  V8H05_RS06025 (V8H05_06025) - 1200821..1201222 (+) 402 WP_000695761.1 hypothetical protein -
  V8H05_RS06030 (V8H05_06030) - 1201219..1201503 (+) 285 WP_001105618.1 hypothetical protein -
  V8H05_RS06035 (V8H05_06035) - 1201496..1201744 (+) 249 WP_338616743.1 DUF1024 family protein -
  V8H05_RS06040 (V8H05_06040) - 1201737..1202246 (+) 510 WP_000185649.1 dUTP pyrophosphatase -
  V8H05_RS06045 (V8H05_06045) - 1202283..1202489 (+) 207 WP_044120006.1 DUF1381 domain-containing protein -
  V8H05_RS06050 (V8H05_06050) - 1202486..1202689 (+) 204 WP_001072794.1 hypothetical protein -
  V8H05_RS06055 (V8H05_06055) - 1202682..1202918 (+) 237 WP_000608278.1 hypothetical protein -
  V8H05_RS06060 (V8H05_06060) - 1202908..1203297 (+) 390 WP_171019343.1 hypothetical protein -
  V8H05_RS06065 (V8H05_06065) - 1203294..1203467 (+) 174 WP_000595243.1 transcriptional activator RinB -
  V8H05_RS06070 (V8H05_06070) - 1203468..1203614 (+) 147 WP_000989999.1 hypothetical protein -
  V8H05_RS06075 (V8H05_06075) - 1203629..1204048 (+) 420 WP_000058632.1 transcriptional regulator -
  V8H05_RS06080 (V8H05_06080) - 1204235..1204675 (+) 441 WP_001003271.1 terminase small subunit -
  V8H05_RS06085 (V8H05_06085) - 1204662..1205939 (+) 1278 WP_000169944.1 PBSX family phage terminase large subunit -
  V8H05_RS06090 (V8H05_06090) - 1205950..1207485 (+) 1536 WP_000921895.1 phage portal protein -
  V8H05_RS06095 (V8H05_06095) - 1207492..1208487 (+) 996 WP_001824750.1 minor capsid protein -
  V8H05_RS06100 (V8H05_06100) - 1208560..1208640 (+) 81 Protein_1196 hypothetical protein -
  V8H05_RS06105 (V8H05_06105) - 1208865..1209479 (+) 615 WP_000354306.1 DUF4355 domain-containing protein -
  V8H05_RS06110 (V8H05_06110) - 1209493..1210467 (+) 975 WP_338616755.1 phage major capsid protein -
  V8H05_RS06115 (V8H05_06115) - 1210489..1210776 (+) 288 WP_001114086.1 hypothetical protein -
  V8H05_RS06120 (V8H05_06120) - 1210785..1211117 (+) 333 WP_000208960.1 phage head-tail connector protein -
  V8H05_RS06125 (V8H05_06125) - 1211114..1211416 (+) 303 WP_001268307.1 hypothetical protein -
  V8H05_RS06130 (V8H05_06130) - 1211416..1211763 (+) 348 WP_001017813.1 HK97-gp10 family putative phage morphogenesis protein -
  V8H05_RS06135 (V8H05_06135) - 1211775..1212158 (+) 384 WP_000188654.1 hypothetical protein -
  V8H05_RS06140 (V8H05_06140) - 1212177..1212758 (+) 582 WP_000002580.1 phage major tail protein, TP901-1 family -
  V8H05_RS06145 (V8H05_06145) - 1212822..1213187 (+) 366 WP_001100168.1 tail assembly chaperone -
  V8H05_RS06150 (V8H05_06150) - 1213217..1213561 (+) 345 WP_000105584.1 hypothetical protein -
  V8H05_RS06155 (V8H05_06155) - 1213578..1217042 (+) 3465 WP_000141461.1 hypothetical protein -
  V8H05_RS06160 (V8H05_06160) - 1217055..1218002 (+) 948 WP_000350680.1 phage tail family protein -
  V8H05_RS06165 (V8H05_06165) - 1218011..1219912 (+) 1902 WP_000156403.1 SGNH/GDSL hydrolase family protein -
  V8H05_RS06170 (V8H05_06170) - 1219927..1221837 (+) 1911 WP_338616763.1 hypothetical protein -
  V8H05_RS06175 (V8H05_06175) - 1221837..1223660 (+) 1824 WP_338616765.1 BppU family phage baseplate upper protein -
  V8H05_RS06180 (V8H05_06180) - 1223660..1224037 (+) 378 WP_112366597.1 DUF2977 domain-containing protein -
  V8H05_RS06185 (V8H05_06185) - 1224041..1224214 (+) 174 WP_000782199.1 XkdX family protein -
  V8H05_RS06190 (V8H05_06190) - 1224254..1224553 (+) 300 WP_001817316.1 DUF2951 domain-containing protein -
  V8H05_RS06195 (V8H05_06195) - 1224690..1226588 (+) 1899 WP_000524011.1 glucosaminidase domain-containing protein -
  V8H05_RS06200 (V8H05_06200) - 1226601..1227839 (+) 1239 WP_000276632.1 BppU family phage baseplate upper protein -
  V8H05_RS06205 (V8H05_06205) - 1227844..1228239 (+) 396 WP_000398880.1 hypothetical protein -
  V8H05_RS06210 (V8H05_06210) - 1228295..1228732 (+) 438 WP_000354133.1 phage holin -
  V8H05_RS06215 (V8H05_06215) - 1228713..1230158 (+) 1446 WP_001148120.1 SH3 domain-containing protein -
  V8H05_RS06220 (V8H05_06220) - 1230555..1230866 (+) 312 WP_000344116.1 DUF3467 domain-containing protein -
  V8H05_RS06225 (V8H05_06225) - 1230853..1231236 (+) 384 WP_001251275.1 hypothetical protein -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 16059.69 Da        Isoelectric Point: 5.8698

>NTDB_id=942042 V8H05_RS05960 WP_000934793.1 1195024..1195452(+) (ssbA) [Staphylococcus aureus strain KWT 2019-23549]
MLNRVVLVGRLTKDPEFRTTPNGVSVATFTLAVNRTFTNAQGEREADFINCVTFRKQADNVNNYLSKGSLAGVDGRLQSR
SYENQEGRRVFVTEVVCDSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTIAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=942042 V8H05_RS05960 WP_000934793.1 1195024..1195452(+) (ssbA) [Staphylococcus aureus strain KWT 2019-23549]
ATGTTAAACAGAGTAGTTTTAGTAGGACGCTTAACAAAAGACCCAGAATTCAGAACAACGCCAAACGGTGTAAGTGTAGC
TACTTTCACTCTTGCAGTCAATAGAACATTCACAAACGCACAAGGAGAACGTGAGGCAGATTTTATTAATTGTGTAACTT
TTAGAAAACAAGCAGATAACGTGAATAACTATTTATCAAAAGGATCATTAGCTGGTGTTGACGGACGCCTACAATCACGT
AGTTATGAAAATCAAGAAGGTCGTCGTGTATTCGTTACCGAAGTTGTATGTGACAGTGTCCAATTCCTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCATTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6B3IY96

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

59.884

100

0.725

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

74.648

0.451

  ssbA Streptococcus mutans UA159

40.141

100

0.401

  ssbB Streptococcus sobrinus strain NIDR 6715-7

39.86

100

0.401

  ssbB/cilA Streptococcus mitis NCTC 12261

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae TIGR4

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae Rx1

38.732

100

0.387

  ssbB/cilA Streptococcus pneumoniae D39

38.732

100

0.387

  ssbB/cilA Streptococcus pneumoniae R6

38.732

100

0.387

  ssbB/cilA Streptococcus mitis SK321

38.732

100

0.387