Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   SA496_RS02405 Genome accession   NZ_CP143312
Coordinates   482639..483856 (-) Length   405 a.a.
NCBI ID   WP_330115005.1    Uniprot ID   -
Organism   Pseudomonas sp. JS3066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 477639..488856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA496_RS02360 (SA496_02360) - 477739..477912 (-) 174 WP_120652969.1 DUF3094 family protein -
  SA496_RS02365 (SA496_02365) - 478001..478627 (-) 627 WP_120652968.1 DUF1780 domain-containing protein -
  SA496_RS02370 (SA496_02370) - 478763..479185 (+) 423 WP_120652967.1 GNAT family N-acetyltransferase -
  SA496_RS02375 (SA496_02375) - 479190..479657 (+) 468 WP_153921520.1 FAD/FMN-containing dehydrogenase -
  SA496_RS02380 (SA496_02380) - 479654..480343 (+) 690 WP_330115002.1 energy-coupling factor ABC transporter permease -
  SA496_RS02385 (SA496_02385) - 480622..480837 (+) 216 WP_330115003.1 hypothetical protein -
  SA496_RS02390 (SA496_02390) yacG 480958..481158 (-) 201 WP_120652963.1 DNA gyrase inhibitor YacG -
  SA496_RS02395 (SA496_02395) coaE 481155..481766 (-) 612 WP_120652962.1 dephospho-CoA kinase -
  SA496_RS02400 (SA496_02400) pilD 481763..482635 (-) 873 WP_330115004.1 A24 family peptidase Machinery gene
  SA496_RS02405 (SA496_02405) pilC 482639..483856 (-) 1218 WP_330115005.1 type II secretion system F family protein Machinery gene
  SA496_RS02410 (SA496_02410) pilB 483859..485565 (-) 1707 WP_330115006.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SA496_RS02415 (SA496_02415) - 485683..486135 (-) 453 WP_330115007.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SA496_RS02425 (SA496_02425) - 486584..486811 (-) 228 WP_330115008.1 hypothetical protein -
  SA496_RS02430 (SA496_02430) nadC 487214..488062 (-) 849 WP_330115009.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44009.80 Da        Isoelectric Point: 9.8159

>NTDB_id=928636 SA496_RS02405 WP_330115005.1 482639..483856(-) (pilC) [Pseudomonas sp. JS3066]
MADKALKNSTFAWEGTDKKGSKIRGEISGQNPALVKAQLRKQGINPTKVRKKSASLLSAGKKIKPMDIALFTRQMATMMK
AGVPLMQSFDIIGEGFEKPAMRKLVEEVKQEVAAGNSLANSLRKKPDYFDDLYCNLVDSGEQAGALETLLDRIATYKEKT
EALKAKIKKAMNYPIAVIVVAIIVTAILLIKVVPQFQSVFANFGAELPAFTLFVIGISEILQEWWFIVLAGLFAFAYVAQ
QAHKKSQKFRDWVDRTVLKLPIFGDILYKSAVARFARTLSTTFAAGVPLVDALDSVAGASGNVVFKNAVIKVKSDVSTGM
QLNFSMRTTGIFPAMAVQMTAIGEESGALDEMLDKVASFYEAEVDNAVDGLTSLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=928636 SA496_RS02405 WP_330115005.1 482639..483856(-) (pilC) [Pseudomonas sp. JS3066]
ATGGCGGACAAAGCGCTAAAAAACAGTACCTTCGCCTGGGAAGGTACCGATAAGAAAGGCTCCAAGATCAGAGGCGAAAT
CAGTGGGCAGAACCCCGCCTTGGTCAAGGCGCAGCTGCGCAAGCAGGGCATCAACCCTACCAAGGTGCGCAAGAAGTCGG
CCTCTCTGCTGAGCGCAGGGAAAAAGATCAAGCCCATGGACATCGCCCTCTTCACGCGGCAGATGGCGACCATGATGAAA
GCTGGTGTGCCACTGATGCAGTCTTTCGACATCATTGGCGAAGGCTTCGAGAAACCGGCCATGCGCAAACTGGTCGAGGA
GGTCAAACAGGAGGTTGCTGCGGGCAACAGTCTGGCTAACTCTCTGCGCAAGAAGCCTGATTACTTTGACGATCTCTATT
GCAACCTTGTCGACTCGGGCGAACAAGCCGGCGCCCTGGAAACCTTGCTGGACCGGATCGCTACCTACAAGGAAAAAACC
GAGGCGCTGAAAGCCAAGATTAAGAAAGCCATGAACTACCCCATTGCAGTGATAGTAGTGGCCATCATCGTCACCGCTAT
TCTACTGATCAAGGTCGTCCCCCAGTTCCAATCGGTGTTCGCCAACTTTGGCGCCGAACTCCCCGCCTTCACCTTGTTTG
TAATTGGAATATCCGAAATCCTTCAAGAGTGGTGGTTCATTGTACTTGCTGGACTATTTGCATTCGCCTACGTCGCGCAG
CAAGCACATAAGAAATCGCAGAAGTTTCGCGATTGGGTTGATCGTACAGTTCTCAAGTTGCCTATATTCGGTGACATCCT
CTACAAATCAGCGGTAGCTCGCTTTGCACGGACGCTTTCCACAACATTCGCGGCAGGGGTTCCACTTGTAGATGCCCTGG
ACTCCGTAGCCGGCGCCAGCGGTAACGTGGTTTTCAAGAATGCTGTAATCAAGGTCAAATCAGACGTCTCCACCGGCATG
CAGCTGAACTTCTCCATGCGTACCACCGGCATATTTCCGGCTATGGCCGTTCAGATGACCGCCATCGGCGAAGAATCCGG
CGCGCTGGATGAAATGCTCGACAAGGTCGCCAGCTTCTACGAAGCCGAAGTGGATAACGCCGTTGATGGCCTGACATCCC
TAATGGAGCCAATGATCATGGCCGTCCTCGGCGTACTGGTCGGCGGCCTGATCATCGCGATGTACCTTCCCATCTTCCAA
TTGGGCGCCGTTGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.222

100

0.822
  pilC Acinetobacter baumannii D1279779

60.891

99.753

0.607
  pilC Acinetobacter baylyi ADP1

60.705

98.025

0.595
  pilC Legionella pneumophila strain ERS1305867

53.713

99.753

0.536
  pilG Neisseria gonorrhoeae MS11

44.527

99.259

0.442
  pilG Neisseria meningitidis 44/76-A

44.527

99.259

0.442
  pilC Vibrio cholerae strain A1552

40.988

100

0.41
  pilC Vibrio campbellii strain DS40M4

39.9

99.012

0.395
  pilC Thermus thermophilus HB27

36.5

98.765

0.36