Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SA496_RS02410 Genome accession   NZ_CP143312
Coordinates   483859..485565 (-) Length   568 a.a.
NCBI ID   WP_330115006.1    Uniprot ID   -
Organism   Pseudomonas sp. JS3066     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 478859..490565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SA496_RS02375 (SA496_02375) - 479190..479657 (+) 468 WP_153921520.1 FAD/FMN-containing dehydrogenase -
  SA496_RS02380 (SA496_02380) - 479654..480343 (+) 690 WP_330115002.1 energy-coupling factor ABC transporter permease -
  SA496_RS02385 (SA496_02385) - 480622..480837 (+) 216 WP_330115003.1 hypothetical protein -
  SA496_RS02390 (SA496_02390) yacG 480958..481158 (-) 201 WP_120652963.1 DNA gyrase inhibitor YacG -
  SA496_RS02395 (SA496_02395) coaE 481155..481766 (-) 612 WP_120652962.1 dephospho-CoA kinase -
  SA496_RS02400 (SA496_02400) pilD 481763..482635 (-) 873 WP_330115004.1 A24 family peptidase Machinery gene
  SA496_RS02405 (SA496_02405) pilC 482639..483856 (-) 1218 WP_330115005.1 type II secretion system F family protein Machinery gene
  SA496_RS02410 (SA496_02410) pilB 483859..485565 (-) 1707 WP_330115006.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SA496_RS02415 (SA496_02415) - 485683..486135 (-) 453 WP_330115007.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  SA496_RS02425 (SA496_02425) - 486584..486811 (-) 228 WP_330115008.1 hypothetical protein -
  SA496_RS02430 (SA496_02430) nadC 487214..488062 (-) 849 WP_330115009.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62679.76 Da        Isoelectric Point: 5.5862

>NTDB_id=928637 SA496_RS02410 WP_330115006.1 483859..485565(-) (pilB) [Pseudomonas sp. JS3066]
MTEIAQTLSGLAKQLVVANLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAAEQFGVAFLDLGSVDKDSQPK
DLVSEKLIRQHRALPLWRRGNKLFVAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDSATSGMEDLGDVDL
DGVDVEAVDDDKKDSAGGVDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERTYRVRFRTDGMLHEVAKPPIQLASRISA
RLKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLSALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRMRNMGVPAFNIATSVNLIIAQRLARKLCSHCKKELDVPHETLIKEGF
PEARIGTFKLYGPVGCENCNQGYKGRVGIYEVVKITPAMQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=928637 SA496_RS02410 WP_330115006.1 483859..485565(-) (pilB) [Pseudomonas sp. JS3066]
ATGACAGAAATTGCCCAAACCCTTTCAGGCCTAGCCAAGCAATTGGTAGTGGCCAATCTGTTGGATGAACGAAGCGCCCT
ACAGGCTCATACTCAAGCGCAGCGCAACAAGATTCCTCTGATCACTTATCTCGTTCAAAACAAGCTACTGAAAAGCCGCG
ACCTAGCGGAAATGGCGGCAGAGCAGTTCGGGGTAGCCTTCCTCGATCTTGGCAGCGTCGACAAGGATAGCCAGCCCAAG
GACCTCGTCAGTGAAAAGCTGATTCGCCAACACCGCGCCCTGCCCCTCTGGCGTCGCGGCAACAAGCTCTTCGTCGCCAT
TTCCGACCCCACCAATCACCAGGCTGTTACCGACATCCAGTTCAGCACCGGCCTCAATACTGAGGCCATCCTGGTTGAGG
ATGACAAGCTGGGCGATGCCATCGAGAAATTCTTCGACAGCGCCACCAGCGGCATGGAGGATCTGGGCGATGTCGATCTT
GATGGCGTAGATGTTGAAGCGGTCGATGATGACAAAAAGGACAGCGCGGGAGGCGTGGACGCCGACGATGCCCCCGTGGT
GCGTTTTGTCAACAAGATGCTGCTGGATGCCATCCGGGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAACGCACCT
ACCGCGTACGCTTCCGTACCGACGGCATGTTGCATGAAGTGGCCAAGCCACCGATCCAGCTGGCCAGCCGGATTTCCGCC
CGCCTGAAGGTAATGGCCGCCCTGGATATTTCCGAGCGCCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGATTTCCAA
GACGAAATCCATCGATTTCCGCGTCAACACCCTGCCTACACTCTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCA
CCAGTGCGCAGATGGGGATCGATGCATTGGGCTATGAGGAAGAACAGAAAGAACTCTACCTCTCAGCTCTGAAGCAACCC
CAAGGTATGATCCTGGTAACCGGCCCTACCGGTTCCGGCAAGACGGTTTCCCTGTATACCGGCCTGAACATCCTCAACAC
CGTTGATATCAACATTTCCACGGCTGAAGACCCCGTGGAGATCAACCTCGAAGGCATCAACCAAGTCAACGTCAACCCCA
AACAAGGCATGGACTTCGCCCAGGCTCTTCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGTGAGATTCGT
GACCTGGAAACTGCCGAAATCGCCATCAAGGCGGCCCAGACCGGCCACATGGTGCTGTCCACCCTGCACACCAACAGCGC
TGCTGAAACACTGACGCGCATGCGCAACATGGGAGTGCCCGCGTTCAACATCGCCACCTCGGTCAACTTGATCATCGCCC
AACGCCTGGCACGCAAATTGTGTAGCCATTGCAAGAAGGAATTGGACGTCCCACACGAAACACTGATCAAGGAAGGCTTC
CCTGAGGCCAGAATTGGCACCTTCAAGCTCTATGGGCCGGTCGGCTGCGAAAACTGCAACCAGGGATACAAAGGCCGAGT
GGGTATTTATGAAGTGGTTAAAATCACGCCGGCCATGCAGCAGCTTATTATGGAAGAAGGTAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGCGATGGCTTCAATGACCTGCGAACCTCGGGCCTGGTGAAAGCCATGAATGGCATCACCAGCCTGGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.923

100

0.579
  pilB Acinetobacter baylyi ADP1

58.259

99.12

0.577
  pilB Acinetobacter baumannii D1279779

57.979

99.296

0.576
  pilF Neisseria gonorrhoeae MS11

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

49.023

99.12

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

51.02

94.894

0.484
  pilB Vibrio cholerae strain A1552

52.29

92.254

0.482
  pilF Thermus thermophilus HB27

37.766

99.296

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.134

0.368