Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OCV56_RS13200 Genome accession   NZ_AP025492
Coordinates   2993875..2995104 (+) Length   409 a.a.
NCBI ID   WP_086711970.1    Uniprot ID   A0A5M9N8Y8
Organism   Vibrio gigantis strain LMG 22741     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2988875..3000104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS13180 ampD 2989877..2990476 (-) 600 WP_086711967.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV56_RS13185 nadC 2990560..2991450 (+) 891 WP_086711968.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV56_RS13190 pilA 2991704..2992171 (+) 468 WP_086711969.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OCV56_RS13195 pilB 2992174..2993859 (+) 1686 WP_086712029.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV56_RS13200 pilC 2993875..2995104 (+) 1230 WP_086711970.1 type II secretion system F family protein Machinery gene
  OCV56_RS13205 pilD 2995172..2996041 (+) 870 WP_086711971.1 A24 family peptidase Machinery gene
  OCV56_RS13210 coaE 2996044..2996658 (+) 615 WP_086711972.1 dephospho-CoA kinase -
  OCV56_RS13215 zapD 2996685..2997425 (+) 741 WP_086711973.1 cell division protein ZapD -
  OCV56_RS13220 yacG 2997506..2997703 (+) 198 WP_019823814.1 DNA gyrase inhibitor YacG -
  OCV56_RS13225 rplS 2998012..2998365 (-) 354 WP_004735514.1 50S ribosomal protein L19 -
  OCV56_RS13230 trmD 2998425..2999165 (-) 741 WP_086711974.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  OCV56_RS13235 rimM 2999192..2999746 (-) 555 WP_048615624.1 ribosome maturation factor RimM -
  OCV56_RS13240 rpsP 2999771..3000019 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45295.32 Da        Isoelectric Point: 10.1285

>NTDB_id=92099 OCV56_RS13200 WP_086711970.1 2993875..2995104(+) (pilC) [Vibrio gigantis strain LMG 22741]
MSSKSKQSQLKSYHWKGINSSGKKVSGQTLALTELEVREKLKEQHIQIKKIKKKSISAVTRLTHRVKAKDITILTRQLAT
MLATGVPIVQAIKLVSDNHRKAEMKSILSHICKGVEAGTPISKAMRTASRHFDDLYTDLVATGELSGNLAQVFERLATYR
EKSEQLKSKVVKALIYPAMVVTVALTVSYLMLTMVIPEFESMFSGFGADLPWFTQQVLYLSHWMQAYSFYAAVSIGLLVL
IIYQLCQRSHSFRLSVSRLGLRFPVLGGVLAKASIAKFSRTLSTSFSSGIPILMSLKTTAKTSGNLHYESAIIEVHRETA
AGMPMYIAMRNTNAFPEMVLQMVMIGEESGNLDDMLNKVASIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLP
IFNLMSVLG

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=92099 OCV56_RS13200 WP_086711970.1 2993875..2995104(+) (pilC) [Vibrio gigantis strain LMG 22741]
ATGAGTAGCAAGTCCAAACAATCACAATTAAAAAGCTATCACTGGAAAGGTATTAACAGCTCAGGCAAGAAAGTGTCAGG
ACAAACCTTAGCGCTTACCGAATTAGAAGTGCGAGAAAAGCTCAAAGAACAACATATTCAAATTAAGAAAATCAAAAAGA
AAAGTATTTCAGCCGTCACTCGTTTAACACATCGCGTCAAAGCCAAAGACATCACAATTTTAACTCGGCAACTTGCGACT
ATGCTCGCCACCGGTGTCCCCATCGTACAAGCGATAAAGTTGGTATCAGACAATCACCGTAAAGCAGAAATGAAATCAAT
TTTATCGCACATCTGCAAAGGTGTAGAAGCCGGAACACCAATCTCAAAAGCGATGCGAACCGCAAGCCGCCACTTTGATG
ACCTCTACACCGACTTGGTCGCGACAGGTGAATTGTCCGGTAACCTTGCGCAAGTGTTTGAACGCTTAGCGACTTATCGA
GAAAAGAGCGAGCAACTAAAATCTAAAGTCGTCAAAGCACTGATTTACCCCGCGATGGTGGTCACCGTTGCACTGACCGT
TTCTTATTTAATGCTGACGATGGTCATTCCGGAGTTTGAGTCGATGTTTTCAGGCTTTGGTGCAGACTTACCTTGGTTTA
CTCAGCAAGTACTCTACCTTTCCCATTGGATGCAGGCTTACAGTTTTTATGCGGCTGTAAGCATCGGACTTCTTGTCCTG
ATTATTTACCAACTGTGCCAGCGCTCCCACTCGTTCAGACTGTCTGTCAGTCGGCTAGGATTGCGTTTCCCTGTTCTCGG
TGGCGTATTAGCTAAAGCCTCAATAGCCAAATTCAGCCGAACCTTATCAACAAGCTTTAGTTCCGGTATTCCTATTTTAA
TGAGCCTAAAAACCACAGCCAAAACCTCAGGAAACCTGCATTATGAATCAGCAATCATTGAGGTTCACCGAGAAACCGCA
GCAGGCATGCCAATGTATATCGCGATGCGCAATACCAATGCCTTCCCTGAAATGGTGTTGCAGATGGTGATGATTGGTGA
AGAGTCGGGCAACCTTGATGATATGCTCAACAAAGTCGCTTCCATTTACGAGTTTGAAGTAGACAACACCGTCGACAATT
TAGGCAAAATTTTAGAACCATTGATCATCGTATTTTTGGGTACGGTTGTTGGCGGGCTTGTGGTTGCGATGTACTTACCG
ATCTTTAATCTTATGAGTGTGTTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9N8Y8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

80

97.8

0.782
  pilC Vibrio campbellii strain DS40M4

73.892

99.267

0.734
  pilC Acinetobacter baylyi ADP1

41.809

100

0.418
  pilC Acinetobacter baumannii D1279779

41.832

98.778

0.413
  pilC Legionella pneumophila strain ERS1305867

42.569

97.066

0.413
  pilC Pseudomonas stutzeri DSM 10701

40.657

96.822

0.394
  pilG Neisseria gonorrhoeae MS11

39.348

97.555

0.384
  pilG Neisseria meningitidis 44/76-A

39.098

97.555

0.381

Multiple sequence alignment