Detailed information NTG0092100

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: OCV56_RS13205
• Genome accession: NZ_AP025492
• Coordinates: 2995172..2996041 (+)
• Length: 289 a.a.
• NCBI ID: WP_086711971.1
• Uniprot ID: A0A5M9NFD9
• Organism: Vibrio gigantis strain LMG 22741
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2990172..3001041

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCV56_RS13185	nadC	2990560..2991450 (+)	891	WP_086711968.1	carboxylating nicotinate-nucleotide diphosphorylase	-
OCV56_RS13190	pilA	2991704..2992171 (+)	468	WP_086711969.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
OCV56_RS13195	pilB	2992174..2993859 (+)	1686	WP_086712029.1	type IV-A pilus assembly ATPase PilB	Machinery gene
OCV56_RS13200	pilC	2993875..2995104 (+)	1230	WP_086711970.1	type II secretion system F family protein	Machinery gene
OCV56_RS13205	pilD	2995172..2996041 (+)	870	WP_086711971.1	A24 family peptidase	Machinery gene
OCV56_RS13210	coaE	2996044..2996658 (+)	615	WP_086711972.1	dephospho-CoA kinase	-
OCV56_RS13215	zapD	2996685..2997425 (+)	741	WP_086711973.1	cell division protein ZapD	-
OCV56_RS13220	yacG	2997506..2997703 (+)	198	WP_019823814.1	DNA gyrase inhibitor YacG	-
OCV56_RS13225	rplS	2998012..2998365 (-)	354	WP_004735514.1	50S ribosomal protein L19	-
OCV56_RS13230	trmD	2998425..2999165 (-)	741	WP_086711974.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
OCV56_RS13235	rimM	2999192..2999746 (-)	555	WP_048615624.1	ribosome maturation factor RimM	-
OCV56_RS13240	rpsP	2999771..3000019 (-)	249	WP_004410028.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32553.76 Da
• Isoelectric Point: 6.9291

>NTDB_id=92100 OCV56_RS13205 WP_086711971.1 2995172..2996041(+) (pilD) [Vibrio gigantis strain LMG 22741]
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPVPEGKFNLSIPRSTCPKCETQLRII
DNIPVLSWLFLKGKCHSCASPISARYPLVELLTAILCTVVASHFGFSFYAIALIFFTFALITATFIDLDTMLLPDQITLP
LVWSGIALALFNVSPVSLQDSVVGAMVGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLLWGNDIMGWYFTSVLGI

Nucleotide

• Length: 870 bp

>NTDB_id=92100 OCV56_RS13205 WP_086711971.1 2995172..2996041(+) (pilD) [Vibrio gigantis strain LMG 22741]
ATGGAAGTATTTCACTACTATCCTTGGCTATTCCCCGTATTAGCTTTCATTTTTAGCCTTCTTATCGGCAGCTTCCTCAA
CGTAGTCATACACCGCTTACCGATTATGATGGAGCGAGAGTGGCAACAAGAGTGCTCGGAGTATTTCTCTCAATATAAAA
TCCCAGTACCTGAAGGGAAATTTAACCTAAGTATTCCACGTTCGACTTGCCCGAAATGTGAAACCCAATTAAGAATTATC
GACAATATTCCAGTATTAAGCTGGTTGTTCTTAAAGGGCAAGTGTCACAGTTGTGCTAGTCCAATCAGTGCTCGCTACCC
TTTGGTCGAGCTGCTTACCGCAATCCTTTGTACCGTAGTCGCGAGCCACTTTGGTTTCAGTTTCTACGCTATTGCACTGA
TCTTTTTCACTTTCGCATTGATTACGGCGACCTTTATCGATCTCGACACCATGCTGCTGCCTGATCAAATCACCTTACCT
TTGGTTTGGTCTGGTATCGCTTTAGCACTGTTCAATGTCAGCCCAGTATCGCTGCAAGATTCTGTGGTTGGTGCAATGGT
TGGCTACTTAGCTCTATGGTCTGTTTATTGGCTGTTCAAACTACTAACGGGCAAAGAAGGCATGGGTTACGGTGACTTTA
AACTCTTAGCGGCACTTGGCGCGTGGCTTGGTTGGCAGCATCTACCAATGATCATCCTGTTATCTTCGCTTGTTGGCCTT
GTATTTGGTTTGATTCAACTTCGCTTAAAACAACAAGGCATCGATAAAGCCTTTCCATTTGGTCCCTATCTCGCGATTGC
TGGTTGGGTAAGCCTATTGTGGGGTAACGACATCATGGGTTGGTATTTCACTTCTGTATTAGGAATTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

3D structure

• AlphaFold DB: A0A5M9NFD9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	73.356	100	0.734
pilD	Vibrio cholerae strain A1552	69.097	99.654	0.689
pilD	Acinetobacter nosocomialis M2	51.311	92.388	0.474
pilD	Acinetobacter baumannii D1279779	51.321	91.696	0.471
pilD	Neisseria gonorrhoeae MS11	48.263	89.619	0.433