Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OCV56_RS13195 Genome accession   NZ_AP025492
Coordinates   2992174..2993859 (+) Length   561 a.a.
NCBI ID   WP_086712029.1    Uniprot ID   A0A5M9N7B7
Organism   Vibrio gigantis strain LMG 22741     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2987174..2998859
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV56_RS13175 pdhR 2988632..2989393 (-) 762 WP_029225519.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  OCV56_RS13180 ampD 2989877..2990476 (-) 600 WP_086711967.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  OCV56_RS13185 nadC 2990560..2991450 (+) 891 WP_086711968.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OCV56_RS13190 pilA 2991704..2992171 (+) 468 WP_086711969.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  OCV56_RS13195 pilB 2992174..2993859 (+) 1686 WP_086712029.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OCV56_RS13200 pilC 2993875..2995104 (+) 1230 WP_086711970.1 type II secretion system F family protein Machinery gene
  OCV56_RS13205 pilD 2995172..2996041 (+) 870 WP_086711971.1 A24 family peptidase Machinery gene
  OCV56_RS13210 coaE 2996044..2996658 (+) 615 WP_086711972.1 dephospho-CoA kinase -
  OCV56_RS13215 zapD 2996685..2997425 (+) 741 WP_086711973.1 cell division protein ZapD -
  OCV56_RS13220 yacG 2997506..2997703 (+) 198 WP_019823814.1 DNA gyrase inhibitor YacG -
  OCV56_RS13225 rplS 2998012..2998365 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62215.99 Da        Isoelectric Point: 5.1191

>NTDB_id=92098 OCV56_RS13195 WP_086712029.1 2992174..2993859(+) (pilB) [Vibrio gigantis strain LMG 22741]
MLTNLPTVLRQAGLLSLTQEQAVAEHVQALGVSTPEALLVLDFFTGDSLANNIQTIFGLPLVKLTNTDYEALCDQLGLRE
LITKYRAIPISVSNSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEVSDDEMTSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSDDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKKNDDSPDIFLRHSIPNSQT
IYKATPQGCNECNQGYSGRVGIYEVMPFTDKLKNSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=92098 OCV56_RS13195 WP_086712029.1 2992174..2993859(+) (pilB) [Vibrio gigantis strain LMG 22741]
CTGCTAACCAACCTACCAACCGTTCTACGTCAGGCTGGCTTACTTAGCCTGACTCAAGAACAAGCCGTGGCGGAACATGT
ACAAGCTTTGGGTGTTTCGACACCCGAAGCTTTACTCGTTCTAGATTTCTTCACCGGCGACTCTTTAGCAAACAACATAC
AAACTATTTTCGGGTTACCGTTAGTAAAACTAACCAATACTGACTACGAAGCCTTGTGTGATCAATTAGGGCTTCGTGAA
CTGATTACCAAGTATCGTGCTATCCCTATATCAGTCTCTAATTCTACTCTCACACTGGCCTCAGCCGACCCGACCGATTT
ACAAGCGGAAGATGACTTTCGATTTGCGACGGGATTACAGATCGAACTCGTTGTCGCCAATTATTCTGAGTTAGAAGGCG
CTATACGCAAACTGTATGGCCGCTCCATTTCTGGGCAAGACTCCAAGCGTAAAGAGATCACCCAAGATGAACTCGCCAAT
CTCGTTGAAGTCTCTGACGATGAGATGACTTCAATTGAAGATCTCAGCCAAGACGACTCTCCAGTTAGCCGATTTATCAA
TCAAATACTGGTTGATGCGGTGCGTAAGGGTGCATCAGATATTCATTTTGAACCTTACGAAGAGCACTATCGCGTGCGCC
TGCGTTGCGATGGCATCCTTGTCGAGATTCAGCAACCCGCCTCTCATTTAAGTCGCCGGTTATCTGCTCGCTTGAAGATT
CTCGCCAAGCTTGATATTGCGGAACGTCGCTTACCCCAAGATGGACGCATTAAGCTCCGTTTGAATGATGAACTCGCGAT
TGATATGCGCGTATCAACGCTACCGACTTTGTGGGGGGAAAAGATCGTACTGAGGCTGCTCGATAGCAGTGCGGCCAATC
TCGATATCGACAAGCTTGGTTACAGCGACGATCAAAAAGCTCTCTACCTTAATGCCCTTAAACGCCCACAAGGTATGATC
TTGATGACAGGGCCAACCGGCAGCGGAAAAACCGTCTCCCTATACACAGGCCTTCGAGTACTGAATACCACTGAACGCAA
TATTTCTACAGCTGAAGACCCAGTGGAGATTAACCTGTGTGGTATCAATCAAGTACAGGTCACACCAAAGATCGGTTTCG
GATTCGCTGAAGCACTGCGCTCGTTTTTACGACAAGATCCCGATGTGGTAATGGTGGGTGAGATTCGTGATTTAGAAACC
GCAGAGATCGCCATCAAAGCATCGCAAACCGGTCACTTGGTGCTTTCGACTCTTCATACTAACTCTGCAGCGGAAACCGT
CACTCGGCTCGCCCACATGGGTATTGAACCATTTAACTTAGCGTCCTCATTAAGCTTAATTATCGCTCAGCGACTGGCAA
GACGCTTGTGCAACCATTGCAAAAAAAATGACGACTCTCCGGACATCTTTCTGCGCCACTCTATCCCTAACAGCCAAACC
ATCTATAAAGCCACCCCTCAAGGGTGTAATGAGTGTAATCAAGGCTATTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
TTTTACCGACAAACTCAAAAACAGCCTTATCAATAAACCCAATGCGCTAGCAATTGAAGACCTTGCGCGTCGAGAAGGCA
TGCGAACACTGCAAGAGTCAGGTCTGGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAACTGCAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5M9N7B7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.227

100

0.736
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio parahaemolyticus RIMD 2210633

70.107

100

0.702
  pilB Acinetobacter baumannii D1279779

51.06

92.513

0.472
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilB Legionella pneumophila strain ERS1305867

50.196

90.909

0.456
  pilF Neisseria gonorrhoeae MS11

43.929

99.822

0.439

Multiple sequence alignment