Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VER99_RS12825 Genome accession   NZ_CP141822
Coordinates   2820505..2822208 (-) Length   567 a.a.
NCBI ID   WP_020334613.1    Uniprot ID   -
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2815505..2827208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VER99_RS12805 (VER99_12805) - 2816211..2817050 (-) 840 WP_020334610.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VER99_RS12810 (VER99_12810) - 2817120..2818613 (-) 1494 WP_020334611.1 SPOR domain-containing protein -
  VER99_RS12815 (VER99_12815) aroB 2818666..2819766 (-) 1101 WP_020334612.1 3-dehydroquinate synthase -
  VER99_RS12820 (VER99_12820) aroK 2819798..2820316 (-) 519 WP_014233147.1 shikimate kinase AroK -
  VER99_RS12825 (VER99_12825) pilQ 2820505..2822208 (-) 1704 WP_020334613.1 type IV pilus secretin PilQ Machinery gene
  VER99_RS12830 (VER99_12830) pilP 2822276..2822791 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  VER99_RS12835 (VER99_12835) pilO 2822781..2823374 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  VER99_RS12840 (VER99_12840) pilN 2823367..2823942 (-) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  VER99_RS12845 (VER99_12845) pilM 2823926..2824942 (-) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62067.69 Da        Isoelectric Point: 4.3212

>NTDB_id=919716 VER99_RS12825 WP_020334613.1 2820505..2822208(-) (pilQ) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MLTGLLSFWLVLLFTPFALADGGMSNALKSIDFRTNKDKDAVIVVELSSPAAIVDIQRVQEGLSIDLLNTSVKDEQLYLL
DVKDFSTVVDSVEVFREVSTTRLVAHISDDYTHDYRLAGRYLEITVSKLKSDEKTPDKSILEKEGKLISINFQDIPVRNV
LQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQLEKQQLEEEMGE
LSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLVRELPENILVIRDIIESLDIPVKQVQIEARIVTVS
EGNMDELGIRWGFSSINGNNTVGGSIENNLATIGLYEGDGEEGEGGSVGIDDFLNVNLAATNPSATSIAFQVAKLGSDTL
LDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTITFKKAVLSLKVTPQITPDNRLVLDLSVTQ
DRPGQVVKTGTGEAVTINTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRSYEKVGKSELLIFV
TPKVVIQ

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=919716 VER99_RS12825 WP_020334613.1 2820505..2822208(-) (pilQ) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGCTCACTGGGCTCTTATCCTTTTGGTTAGTTTTACTTTTCACCCCTTTTGCTTTGGCTGATGGCGGGATGTCTAATGC
GTTAAAGAGTATCGACTTTCGCACGAACAAGGACAAAGACGCGGTGATTGTGGTGGAACTGTCTTCACCGGCTGCGATTG
TCGATATTCAGCGGGTTCAGGAAGGACTGAGCATCGACTTGCTTAATACGTCAGTGAAAGATGAGCAGTTATATCTCCTC
GATGTAAAAGACTTTTCAACGGTAGTGGACAGCGTGGAAGTCTTTCGTGAAGTCTCGACGACCCGTTTAGTCGCGCATAT
TAGTGATGATTACACCCATGATTACCGTTTGGCAGGGCGCTATTTGGAAATCACGGTCAGTAAACTCAAGTCTGATGAAA
AAACACCAGATAAGAGCATTTTAGAGAAAGAAGGCAAACTGATATCGATTAACTTCCAGGATATCCCGGTACGTAACGTC
TTACAGCTCATTGCCGACTATAACCAGTTTAACCTGGTGGTATCAGACTCGGTCGAAGGCAACCTGACACTGCGACTTGA
TGGTGTGCCTTGGCAGCAGGTTTTAGACATTATTCTGCAAGTAAAAGGTTTGGATAAGCGAGTTGATGGTAATGTCATTC
TTGTCGCGCCGACGGATGAACTTGACCTTCGAGAGAAGCAGCAACTAGAGAAGCAGCAACTTGAAGAGGAAATGGGCGAA
CTGTCGTCTGAAATCATCAAAGTGAATTTTGCCAAAGCATCCGATATTGCTGAGATGATTAATGGTGAAGGCAATATCAG
CATGCTCTCTGAGCGAGGCAGCATGACGATTGATGAGCGCACTAACTCACTATTGGTCAGAGAATTGCCAGAGAATATCT
TGGTTATTCGTGACATTATTGAGTCACTCGATATTCCGGTTAAGCAGGTCCAAATCGAAGCGAGGATTGTCACCGTCAGT
GAAGGGAACATGGATGAACTCGGCATTCGATGGGGATTCTCTTCCATCAACGGCAATAACACCGTTGGTGGCTCGATTGA
GAATAACCTCGCAACCATTGGCCTTTATGAAGGTGATGGTGAAGAGGGCGAGGGCGGTAGTGTTGGAATTGATGATTTTC
TTAACGTCAACCTGGCCGCTACTAACCCGAGCGCAACCAGCATTGCGTTTCAGGTCGCCAAGTTGGGCTCTGACACTTTG
CTCGATCTGGAACTTTCTGCACTGCAGCAGGAGTCGAAAGCTGAGATCATCTCAAGCCCTCGTTTAATCACTACTAATAA
AAAACCGGCCTATATAGAACAAGGTACAGAAATTCCGTATCTAGAATCATCATCGAGTGGTGCGACAACCATTACGTTCA
AAAAAGCTGTACTAAGTCTGAAAGTCACGCCACAAATTACCCCGGATAACCGTCTGGTGCTGGATTTAAGTGTTACTCAG
GACAGACCGGGGCAAGTGGTAAAAACGGGAACTGGCGAAGCGGTCACAATAAACACCCAGAGAATAGGTACTCAAGTCTT
AGTAAACAACGGTGAGACGGTTGTTCTTGGCGGCATTTTCCAACACAGCATTACCAACTCTGTCGATAAAGTGCCATTAC
TCGGTGATCTGCCGTTATTAGGTGCACTTTTCCGCAGAAGTTACGAAAAAGTTGGCAAAAGTGAACTGCTGATTTTCGTT
ACACCAAAGGTTGTTATTCAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(407-565)

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

89.242

100

0.892
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

75.752

99.647

0.755
  pilQ Vibrio cholerae strain A1552

75.752

99.647

0.755
  pilQ Legionella pneumophila strain ERS1305867

41.091

97.002

0.399
  pilQ Legionella pneumophila strain Lp02

41.091

97.002

0.399
  pilQ Pseudomonas aeruginosa PAK

40.399

97.354

0.393
  pilQ Acinetobacter baumannii D1279779

37.99

100

0.393
  pilQ Acinetobacter baumannii strain A118

37.99

100

0.393
  comQ Acinetobacter baylyi ADP1

37.651

100

0.384