Detailed information NTG0919720

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VER99_RS12845
• Genome accession: NZ_CP141822
• Coordinates: 2823926..2824942 (-)
• Length: 338 a.a.
• NCBI ID: WP_020334617.1
• Uniprot ID: A0AAN0Y4M4
• Organism: Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2818926..2829942

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VER99_RS12820 (VER99_12820)	aroK	2819798..2820316 (-)	519	WP_014233147.1	shikimate kinase AroK	-
VER99_RS12825 (VER99_12825)	pilQ	2820505..2822208 (-)	1704	WP_020334613.1	type IV pilus secretin PilQ	Machinery gene
VER99_RS12830 (VER99_12830)	pilP	2822276..2822791 (-)	516	WP_020334614.1	pilus assembly protein PilP	Machinery gene
VER99_RS12835 (VER99_12835)	pilO	2822781..2823374 (-)	594	WP_020334615.1	type 4a pilus biogenesis protein PilO	Machinery gene
VER99_RS12840 (VER99_12840)	pilN	2823367..2823942 (-)	576	WP_024372660.1	PilN domain-containing protein	Machinery gene
VER99_RS12845 (VER99_12845)	pilM	2823926..2824942 (-)	1017	WP_020334617.1	type IV pilus assembly protein PilM	Machinery gene
VER99_RS12850 (VER99_12850)	-	2825086..2827653 (+)	2568	WP_020334618.1	penicillin-binding protein 1A	-
VER99_RS12855 (VER99_12855)	oxyR	2827717..2828625 (-)	909	WP_014233154.1	DNA-binding transcriptional regulator OxyR	-
VER99_RS12860 (VER99_12860)	-	2828784..2829512 (+)	729	WP_020334619.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37587.78 Da
• Isoelectric Point: 7.2098

>NTDB_id=919720 VER99_RS12845 WP_020334617.1 2823926..2824942(-) (pilM) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MDKRIVTGIDIGHNSLKAVVLKPNGEQFALLGYKEILLKDGIVAENNTINHQEIVNTLKQVKKDLPFGASRVAISVPDNA
VISKKLHIEQDLEENEIEFAVVQAFSHQSPFPVDELSLDFVRLNHEGGLRGPVSYQVFATRKDVVESRVEAIRKSGFKPV
LVEVHSQSLGQIWKLATERFPDKSQYGLLDVGSVSSSFTMFTEQNELFHKEFACGTGSIQCSVQESPLSGEKQAKTSQFN
RQMVDRVKRQIQLYTSINGSQNIKGLWLSGEGSTTPMLAEELSHQLAVECELLNPLGLFEMNVAKRKRRAVEWQNFSTAA
GLAVRGIQWLGGHRATSN

Nucleotide

• Length: 1017 bp

>NTDB_id=919720 VER99_RS12845 WP_020334617.1 2823926..2824942(-) (pilM) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGGATAAGCGAATCGTTACAGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTCGTACTTAAACCCAATGGCGAGCA
ATTCGCCTTGTTGGGTTACAAAGAAATATTACTTAAGGATGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAATACCCTTAAACAGGTGAAAAAAGACCTGCCATTTGGTGCAAGCAGAGTGGCAATTTCAGTGCCTGATAATGCG
GTTATCAGCAAAAAACTGCACATCGAACAAGACCTTGAAGAGAACGAAATAGAGTTCGCGGTCGTGCAGGCATTTTCTCA
TCAATCTCCTTTCCCCGTAGATGAGTTGAGTCTGGACTTTGTCCGTCTTAATCATGAAGGAGGCCTGCGAGGGCCAGTTA
GCTACCAAGTTTTTGCAACACGTAAAGATGTCGTGGAGAGTCGGGTTGAAGCAATACGTAAATCCGGTTTTAAACCTGTC
CTCGTCGAGGTCCATTCACAAAGCCTCGGGCAGATATGGAAACTGGCCACAGAACGTTTTCCAGATAAAAGTCAGTACGG
TTTGCTTGATGTTGGTTCTGTGTCGAGCTCTTTCACGATGTTCACCGAGCAAAACGAGCTATTTCATAAAGAATTTGCCT
GTGGAACAGGTTCCATTCAGTGCTCTGTGCAGGAAAGTCCTCTGTCCGGAGAAAAGCAAGCCAAAACGAGTCAATTTAAC
CGTCAGATGGTCGATCGTGTCAAACGTCAGATACAGCTTTATACGTCGATTAATGGGTCACAAAACATCAAAGGTCTTTG
GTTATCCGGTGAAGGGTCAACAACGCCAATGTTGGCTGAGGAGTTATCTCATCAATTGGCGGTCGAGTGCGAGTTACTGA
ATCCTTTAGGCCTATTTGAAATGAACGTCGCTAAACGTAAGCGTCGAGCGGTGGAATGGCAGAATTTTTCAACCGCCGCT
GGCTTGGCAGTACGAGGTATCCAATGGTTGGGAGGTCATCGTGCTACATCGAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-326)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	74.556	100	0.746
pilM	Vibrio cholerae strain A1552	48.521	100	0.485