Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   VER99_RS12835 Genome accession   NZ_CP141822
Coordinates   2822781..2823374 (-) Length   197 a.a.
NCBI ID   WP_020334615.1    Uniprot ID   A0AAN1CWF1
Organism   Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2817781..2828374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VER99_RS12815 (VER99_12815) aroB 2818666..2819766 (-) 1101 WP_020334612.1 3-dehydroquinate synthase -
  VER99_RS12820 (VER99_12820) aroK 2819798..2820316 (-) 519 WP_014233147.1 shikimate kinase AroK -
  VER99_RS12825 (VER99_12825) pilQ 2820505..2822208 (-) 1704 WP_020334613.1 type IV pilus secretin PilQ Machinery gene
  VER99_RS12830 (VER99_12830) pilP 2822276..2822791 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  VER99_RS12835 (VER99_12835) pilO 2822781..2823374 (-) 594 WP_020334615.1 type 4a pilus biogenesis protein PilO Machinery gene
  VER99_RS12840 (VER99_12840) pilN 2823367..2823942 (-) 576 WP_024372660.1 PilN domain-containing protein Machinery gene
  VER99_RS12845 (VER99_12845) pilM 2823926..2824942 (-) 1017 WP_020334617.1 type IV pilus assembly protein PilM Machinery gene
  VER99_RS12850 (VER99_12850) - 2825086..2827653 (+) 2568 WP_020334618.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23102.59 Da        Isoelectric Point: 4.7130

>NTDB_id=919718 VER99_RS12835 WP_020334615.1 2822781..2823374(-) (pilO) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
MVNWQDLEMDEIAEWPLIPQLLVISLLILLLQGFGYWLYLVPKEERLNDLIQQEETLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSDHFLYQLPLNIELTGDFHDIGRFSQAIATLPRII
TFQDVTWQRVSQESETLHFRVRAHTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=919718 VER99_RS12835 WP_020334615.1 2822781..2823374(-) (pilO) [Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759 strain ATCC 14048]
ATGGTTAATTGGCAAGATCTGGAAATGGATGAGATTGCTGAGTGGCCTTTAATCCCTCAGTTGCTAGTCATTAGTTTGTT
GATCCTGTTGCTCCAAGGTTTCGGCTATTGGCTGTACTTGGTGCCTAAGGAAGAGCGATTAAATGACCTCATCCAGCAGG
AAGAAACACTCAAAGTTGCGTTACGAATTAAAGCCAACAAGGTTGCCGCATTACCGCAGCTCCAATCTCAGTTAGATGAA
TTGACTACACGTTACGAGTTGTTGTTGCAGCAATTACCCGCCCAGAAAGAGTTAGCCACCATGCTGGCGTCCGTTAATGA
GCTCGGCATAGACAATAAACTGACCTTCACCCGAATTGACTGGGGAGAAAAGCAGTCTGATCACTTTTTGTATCAGCTGC
CACTTAACATCGAGTTAACCGGTGATTTTCATGACATCGGCCGTTTCTCACAAGCGATTGCAACTTTACCACGCATCATC
ACTTTTCAAGATGTGACTTGGCAACGCGTCAGTCAGGAGAGTGAAACGCTGCACTTCCGGGTAAGAGCTCATACCTATCA
GTTCAAGCCAGAGGAAAAGAAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

88.947

96.447

0.858
  pilO Vibrio cholerae strain A1552

70.558

100

0.706