Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN51_RS03060 Genome accession   NZ_CP141727
Coordinates   622400..623344 (-) Length   314 a.a.
NCBI ID   WP_017368744.1    Uniprot ID   -
Organism   Lactococcus formosensis strain M20011502 FF20a2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 617400..628344
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN51_RS03025 (VNN51_03025) - 618004..618873 (+) 870 WP_279359040.1 RluA family pseudouridine synthase -
  VNN51_RS03030 (VNN51_03030) - 618960..619805 (-) 846 WP_017368750.1 zinc ABC transporter substrate-binding protein -
  VNN51_RS03035 (VNN51_03035) comGF 620041..620481 (-) 441 WP_017368749.1 competence type IV pilus minor pilin ComGF -
  VNN51_RS03040 (VNN51_03040) comGE 620462..620695 (-) 234 WP_279359361.1 competence type IV pilus minor pilin ComGE -
  VNN51_RS03045 (VNN51_03045) comGD 620727..621143 (-) 417 WP_279365405.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN51_RS03050 (VNN51_03050) comYC 621118..621423 (-) 306 WP_017368746.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN51_RS03055 (VNN51_03055) comGB 621442..622461 (-) 1020 WP_279360738.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN51_RS03060 (VNN51_03060) comGA 622400..623344 (-) 945 WP_017368744.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN51_RS03065 (VNN51_03065) rpsU 623533..623709 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN51_RS03070 (VNN51_03070) guaB 623834..625315 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN51_RS03075 (VNN51_03075) - 625489..627090 (+) 1602 WP_253008291.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35803.14 Da        Isoelectric Point: 6.7701

>NTDB_id=919034 VNN51_RS03060 WP_017368744.1 622400..623344(-) (comGA) [Lactococcus formosensis strain M20011502 FF20a2]
MIQVLAKTLLQKAVDQMVHDLYLVALEGKYSLYFRTATERRFERELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRFRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVEAEFLQLQVNKVIGNDYDTLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAIAAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=919034 VNN51_RS03060 WP_017368744.1 622400..623344(-) (comGA) [Lactococcus formosensis strain M20011502 FF20a2]
ATGATACAGGTTTTAGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTCCGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGAGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTTCAAGTCAATAAAGTTATAGGAAATGATTACGA
TACCTTGATTAAGCTTTCGCTTCGCCACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAACTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTACTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATCGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTATTGCAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

53.016

100

0.532
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

52.077

99.682

0.519
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.923

99.363

0.516
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae D39

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae R6

51.757

99.682

0.516
  comYA Streptococcus mutans UA159

50.316

100

0.506
  comYA Streptococcus mutans UA140

50.316

100

0.506