Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN51_RS03055 Genome accession   NZ_CP141727
Coordinates   621442..622461 (-) Length   339 a.a.
NCBI ID   WP_279360738.1    Uniprot ID   -
Organism   Lactococcus formosensis strain M20011502 FF20a2     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 616442..627461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN51_RS03025 (VNN51_03025) - 618004..618873 (+) 870 WP_279359040.1 RluA family pseudouridine synthase -
  VNN51_RS03030 (VNN51_03030) - 618960..619805 (-) 846 WP_017368750.1 zinc ABC transporter substrate-binding protein -
  VNN51_RS03035 (VNN51_03035) comGF 620041..620481 (-) 441 WP_017368749.1 competence type IV pilus minor pilin ComGF -
  VNN51_RS03040 (VNN51_03040) comGE 620462..620695 (-) 234 WP_279359361.1 competence type IV pilus minor pilin ComGE -
  VNN51_RS03045 (VNN51_03045) comGD 620727..621143 (-) 417 WP_279365405.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN51_RS03050 (VNN51_03050) comYC 621118..621423 (-) 306 WP_017368746.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN51_RS03055 (VNN51_03055) comGB 621442..622461 (-) 1020 WP_279360738.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN51_RS03060 (VNN51_03060) comGA 622400..623344 (-) 945 WP_017368744.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN51_RS03065 (VNN51_03065) rpsU 623533..623709 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN51_RS03070 (VNN51_03070) guaB 623834..625315 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN51_RS03075 (VNN51_03075) - 625489..627090 (+) 1602 WP_253008291.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39363.97 Da        Isoelectric Point: 9.0696

>NTDB_id=919033 VNN51_RS03055 WP_279360738.1 621442..622461(-) (comGB) [Lactococcus formosensis strain M20011502 FF20a2]
MDLSQLLQLRVKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLNFYEDV
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRRKLIQVSTYPLVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTI
FLGCLFGLLVFFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGVEFLQILDSGQKFEDALRFYPIFTKELALIVEYGELKAKLGQELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=919033 VNN51_RS03055 WP_279360738.1 621442..622461(-) (comGB) [Lactococcus formosensis strain M20011502 FF20a2]
ATGGATTTATCTCAGCTATTGCAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAAACTTATCCAACTCATGAA
TAATCTTTTCAAAAGTGGTTTCCACCTCTCTGAAATTATTGATTTTCTTGAACGGTCGGGGCTAACAGAAGCATATTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAATGGACAAAGTTTCTCTGGTATTTTAGCGAAGCTAAATTTTTATGAGGATGTT
GTGACTCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTACACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAGAAAGCTCATCCAAGTCTCCACTTACCCGCTCGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACTATC
TTTTTAGGTTGCCTCTTCGGTTTGCTTGTTTTCTTTTACCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTGTTGAATTCCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCCCTTCGATTTTACCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGGCTAAGCTAGGGCAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTCGAAAGAGCAATGCAACTTATCCAGCCTATTGTTTTCTTGCTGGTGGCTTTGTTGATT
ATATTAATTTATGCTGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

53.254

99.705

0.531
  comGB/cglB Streptococcus mitis NCTC 12261

51.94

98.82

0.513
  comYB Streptococcus gordonii str. Challis substr. CH1

51.796

98.525

0.51
  comGB/cglB Streptococcus pneumoniae R6

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

98.82

0.507
  comGB/cglB Streptococcus mitis SK321

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae D39

51.343

98.82

0.507
  comYB Streptococcus mutans UA140

51.111

92.92

0.475
  comYB Streptococcus mutans UA159

50.794

92.92

0.472