Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN46_RS03005 Genome accession   NZ_CP141719
Coordinates   609281..610225 (-) Length   314 a.a.
NCBI ID   WP_141921120.1    Uniprot ID   -
Organism   Lactococcus garvieae strain CAHFS22LA20.5H7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 604281..615225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN46_RS02970 (VNN46_02970) - 604889..605758 (+) 870 WP_141921062.1 RluA family pseudouridine synthase -
  VNN46_RS02975 (VNN46_02975) - 605844..606689 (-) 846 WP_003133722.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  VNN46_RS02980 (VNN46_02980) comYF 606922..607362 (-) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN46_RS02985 (VNN46_02985) comGE 607343..607576 (-) 234 WP_260603768.1 competence type IV pilus minor pilin ComGE -
  VNN46_RS02990 (VNN46_02990) comGD 607608..608024 (-) 417 WP_242359187.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN46_RS02995 (VNN46_02995) comYC 607999..608304 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN46_RS03000 (VNN46_03000) comGB 608323..609342 (-) 1020 WP_260603779.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN46_RS03005 (VNN46_03005) comGA 609281..610225 (-) 945 WP_141921120.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN46_RS03010 (VNN46_03010) rpsU 610413..610589 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN46_RS03015 (VNN46_03015) guaB 610712..612193 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN46_RS03020 (VNN46_03020) - 612362..613963 (+) 1602 WP_017370492.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35870.04 Da        Isoelectric Point: 6.5037

>NTDB_id=918808 VNN46_RS03005 WP_141921120.1 609281..610225(-) (comGA) [Lactococcus garvieae strain CAHFS22LA20.5H7]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDFEQGQALIAHMKFLSGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFQNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918808 VNN46_RS03005 WP_141921120.1 609281..610225(-) (comGA) [Lactococcus garvieae strain CAHFS22LA20.5H7]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTACATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTTGATTTTGAACAAGGGCAAGCAC
TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT
TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAGCATTTTCAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACAGTATTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTGCACGG
CTAAAGGAACTTGGGTTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.554

100

0.596
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.603

99.363

0.513
  comYA Streptococcus mutans UA140

51.603

99.363

0.513