Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN46_RS03000 Genome accession   NZ_CP141719
Coordinates   608323..609342 (-) Length   339 a.a.
NCBI ID   WP_260603779.1    Uniprot ID   -
Organism   Lactococcus garvieae strain CAHFS22LA20.5H7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 603323..614342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN46_RS02970 (VNN46_02970) - 604889..605758 (+) 870 WP_141921062.1 RluA family pseudouridine synthase -
  VNN46_RS02975 (VNN46_02975) - 605844..606689 (-) 846 WP_003133722.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  VNN46_RS02980 (VNN46_02980) comYF 606922..607362 (-) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN46_RS02985 (VNN46_02985) comGE 607343..607576 (-) 234 WP_260603768.1 competence type IV pilus minor pilin ComGE -
  VNN46_RS02990 (VNN46_02990) comGD 607608..608024 (-) 417 WP_242359187.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN46_RS02995 (VNN46_02995) comYC 607999..608304 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN46_RS03000 (VNN46_03000) comGB 608323..609342 (-) 1020 WP_260603779.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN46_RS03005 (VNN46_03005) comGA 609281..610225 (-) 945 WP_141921120.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN46_RS03010 (VNN46_03010) rpsU 610413..610589 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN46_RS03015 (VNN46_03015) guaB 610712..612193 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN46_RS03020 (VNN46_03020) - 612362..613963 (+) 1602 WP_017370492.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39418.18 Da        Isoelectric Point: 9.8175

>NTDB_id=918807 VNN46_RS03000 WP_260603779.1 608323..609342(-) (comGB) [Lactococcus garvieae strain CAHFS22LA20.5H7]
MDLSQLLQLRTKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLVFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGCLFSLLVFFYLGKYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFENALRLYPIFTKELALIVEYGELKSKLGKELMVFSEESWALFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918807 VNN46_RS03000 WP_260603779.1 608323..609342(-) (comGB) [Lactococcus garvieae strain CAHFS22LA20.5H7]
ATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAACTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACATTAGGGCTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGTATTTCTAATCTTTATCATGCTAG
GGTTAAAAAATTATTTACTGCCCCAATTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACCCTC
TTTTTAGGATGCCTCTTCAGTTTGCTTGTTTTCTTTTATCTGGGCAAATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGCTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAAACGCCCTTCGACTTTACCCCATATTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGGCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

54.438

99.705

0.543
  comGB/cglB Streptococcus mitis NCTC 12261

53.134

98.82

0.525
  comGB/cglB Streptococcus pneumoniae D39

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae R6

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae TIGR4

52.836

98.82

0.522
  comGB/cglB Streptococcus mitis SK321

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae Rx1

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.083

99.115

0.516
  comYB Streptococcus mutans UA140

51.582

93.215

0.481
  comYB Streptococcus mutans UA159

51.266

93.215

0.478