Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VNN46_RS02980 Genome accession   NZ_CP141719
Coordinates   606922..607362 (-) Length   146 a.a.
NCBI ID   WP_014024179.1    Uniprot ID   -
Organism   Lactococcus garvieae strain CAHFS22LA20.5H7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 601922..612362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN46_RS02965 (VNN46_02965) - 602525..604813 (-) 2289 WP_141921061.1 PBP1A family penicillin-binding protein -
  VNN46_RS02970 (VNN46_02970) - 604889..605758 (+) 870 WP_141921062.1 RluA family pseudouridine synthase -
  VNN46_RS02975 (VNN46_02975) - 605844..606689 (-) 846 WP_003133722.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  VNN46_RS02980 (VNN46_02980) comYF 606922..607362 (-) 441 WP_014024179.1 competence type IV pilus minor pilin ComGF Machinery gene
  VNN46_RS02985 (VNN46_02985) comGE 607343..607576 (-) 234 WP_260603768.1 competence type IV pilus minor pilin ComGE -
  VNN46_RS02990 (VNN46_02990) comGD 607608..608024 (-) 417 WP_242359187.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN46_RS02995 (VNN46_02995) comYC 607999..608304 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN46_RS03000 (VNN46_03000) comGB 608323..609342 (-) 1020 WP_260603779.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN46_RS03005 (VNN46_03005) comGA 609281..610225 (-) 945 WP_141921120.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN46_RS03010 (VNN46_03010) rpsU 610413..610589 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN46_RS03015 (VNN46_03015) guaB 610712..612193 (-) 1482 WP_003133714.1 IMP dehydrogenase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 16835.45 Da        Isoelectric Point: 9.6906

>NTDB_id=918804 VNN46_RS02980 WP_014024179.1 606922..607362(-) (comYF) [Lactococcus garvieae strain CAHFS22LA20.5H7]
MFLKKNKVTAFTLLESLLALLVLVGTFSLFLGMTKMFHEEVKRATTDHTQDWQLFCSLLRSELEGASLDKVENNYLYVRK
HVNLRFGLSSQGDFRKTNANGRGYQPMIHHLKNAKISQEGEQIKIILTFEKGGDRTFLYTFPEKES

Nucleotide


Download         Length: 441 bp        

>NTDB_id=918804 VNN46_RS02980 WP_014024179.1 606922..607362(-) (comYF) [Lactococcus garvieae strain CAHFS22LA20.5H7]
TTGTTCTTGAAAAAAAATAAAGTTACAGCCTTTACACTCTTGGAATCACTCCTTGCTTTACTCGTGTTAGTGGGCACTTT
TTCTCTTTTTCTAGGTATGACTAAAATGTTTCATGAGGAAGTGAAACGTGCTACAACAGACCATACCCAAGACTGGCAAC
TTTTTTGTAGTCTACTGAGAAGTGAACTTGAGGGAGCAAGTTTAGATAAGGTAGAAAATAACTATCTTTATGTACGCAAA
CATGTTAACCTAAGGTTTGGCCTTTCCTCTCAAGGGGATTTTCGAAAAACTAACGCGAATGGACGAGGCTATCAGCCCAT
GATTCATCATTTAAAAAATGCAAAAATCAGCCAAGAGGGAGAGCAGATAAAAATTATCTTGACTTTTGAAAAAGGAGGAG
ACCGCACATTTCTTTACACATTCCCAGAGAAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(40-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

50.355

96.575

0.486
  comYF Streptococcus mutans UA159

49.645

96.575

0.479
  comGF Lactococcus lactis subsp. cremoris KW2

49.64

95.205

0.473
  comGF/cglF Streptococcus pneumoniae TIGR4

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae D39

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae Rx1

40.972

98.63

0.404
  comGF/cglF Streptococcus pneumoniae R6

40.972

98.63

0.404
  comGF/cglF Streptococcus mitis NCTC 12261

38.889

98.63

0.384
  comGF/cglF Streptococcus mitis SK321

38.194

98.63

0.377