NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	VNN32_RS04725	Genome accession	NZ_CP141703
Coordinates	885239..886183 (+)	Length	314 a.a.
NCBI ID	WP_046401060.1	Uniprot ID	-
Organism	Lactococcus petauri strain R21-74 AL-1
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 880239..891183

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VNN32_RS04710 (VNN32_04705)	-	881500..883101 (-)	1602	WP_338976408.1	GH25 family lysozyme	-
VNN32_RS04715 (VNN32_04710)	guaB	883270..884751 (+)	1482	WP_003133714.1	IMP dehydrogenase	-
VNN32_RS04720 (VNN32_04715)	rpsU	884877..885053 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
VNN32_RS04725 (VNN32_04720)	comGA	885239..886183 (+)	945	WP_046401060.1	competence type IV pilus ATPase ComGA	Machinery gene
VNN32_RS04730 (VNN32_04725)	comGB	886122..887141 (+)	1020	Protein_878	competence type IV pilus assembly protein ComGB	-
VNN32_RS04735 (VNN32_04730)	comYC	887160..887465 (+)	306	WP_029343537.1	competence type IV pilus major pilin ComGC	Machinery gene
VNN32_RS04740 (VNN32_04735)	comGD	887440..887856 (+)	417	WP_019336004.1	competence type IV pilus minor pilin ComGD	Machinery gene
VNN32_RS04745 (VNN32_04740)	comGE	887888..888121 (+)	234	WP_272945968.1	competence type IV pilus minor pilin ComGE	-
VNN32_RS04750 (VNN32_04745)	comGF	888102..888542 (+)	441	WP_019336002.1	competence type IV pilus minor pilin ComGF	-
VNN32_RS04755 (VNN32_04750)	-	888774..889619 (+)	846	WP_019336001.1	zinc ABC transporter substrate-binding protein	-
VNN32_RS04760 (VNN32_04755)	-	889690..890574 (-)	885	WP_019336000.1	RluA family pseudouridine synthase	-

Sequence

Protein

Download Length: 314 a.a. Molecular weight: 35767.20 Da Isoelectric Point: 7.5089

                                >NTDB_id=918575 VNN32_RS04725 WP_046401060.1 885239..886183(+) (comGA) [Lactococcus petauri strain R21-74 AL-1]

MIQVLAKKLLQKAVDQMVHDLYLVALEGKYSLYFRTATERRFERELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI

LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI

CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR

LKELGLSDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNDQIDQLVADGFISAVAAAREKIEFSKAD

Nucleotide

Download Length: 945 bp

                                >NTDB_id=918575 VNN32_RS04725 WP_046401060.1 885239..886183(+) (comGA) [Lactococcus petauri strain R21-74 AL-1]

ATGATACAGGTTTTGGCAAAAAAACTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA

AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCCTTGAGCAAGGGCAAGCAC

TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC

TTAGATAAAGGTGAGCAGCGCTTACGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT

GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT

TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT

TGTATAGAAGATCCTGTGGAGTTAGTAGAAACTGAATTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTATGA

TGCCTTGATTAAGCTTTCGCTGCGACACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG

CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC

TTAAAAGAACTTGGATTAAGTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA

GGGATTGCTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGACCAGATTGATCAGTTGGTTG

CAGATGGATTTATCTCAGCTGTTGCAGCTGCGCGCGAAAAAATTGAGTTTAGCAAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-264)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Lactococcus lactis subsp. cremoris KW2	60.191	100	0.602
comYA	Streptococcus gordonii str. Challis substr. CH1	53.333	100	0.535
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	52.716	99.682	0.525
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	52.564	99.363	0.522
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	52.396	99.682	0.522
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	52.396	99.682	0.522
comGA/cglA/cilD	Streptococcus pneumoniae D39	52.396	99.682	0.522
comGA/cglA/cilD	Streptococcus pneumoniae R6	52.396	99.682	0.522
comYA	Streptococcus mutans UA159	51.266	100	0.516
comYA	Streptococcus mutans UA140	51.266	100	0.516

Detailed information NTG0918575