Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   VNN27_RS10085 Genome accession   NZ_CP141700
Coordinates   2074425..2074841 (-) Length   138 a.a.
NCBI ID   WP_346350003.1    Uniprot ID   -
Organism   Lactococcus petauri strain R22-25 IC35     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2042497..2082133 2074425..2074841 within 0


Gene organization within MGE regions


Location: 2042497..2082133
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN27_RS09860 (VNN27_09860) - 2042497..2042670 (-) 174 WP_276414265.1 hypothetical protein -
  VNN27_RS09870 (VNN27_09870) - 2043764..2044570 (-) 807 WP_019293170.1 DUF1828 domain-containing protein -
  VNN27_RS09875 (VNN27_09875) - 2044574..2045038 (-) 465 WP_346349973.1 hypothetical protein -
  VNN27_RS09880 (VNN27_09880) - 2045297..2045611 (+) 315 WP_346349974.1 hypothetical protein -
  VNN27_RS09885 (VNN27_09885) - 2045669..2046079 (-) 411 WP_346349975.1 hypothetical protein -
  VNN27_RS09890 (VNN27_09890) - 2046276..2046470 (-) 195 WP_346349976.1 hypothetical protein -
  VNN27_RS09895 (VNN27_09895) - 2046945..2047127 (+) 183 WP_346349977.1 hypothetical protein -
  VNN27_RS09900 (VNN27_09900) - 2047254..2047544 (-) 291 WP_346349978.1 YjcQ family protein -
  VNN27_RS09905 (VNN27_09905) - 2047816..2048181 (-) 366 WP_285122058.1 hypothetical protein -
  VNN27_RS09910 (VNN27_09910) - 2048310..2048867 (-) 558 WP_285122057.1 hypothetical protein -
  VNN27_RS09915 (VNN27_09915) - 2048951..2050012 (-) 1062 WP_346349979.1 LysM peptidoglycan-binding domain-containing protein -
  VNN27_RS09920 (VNN27_09920) - 2049996..2050358 (-) 363 WP_019299702.1 hypothetical protein -
  VNN27_RS09925 (VNN27_09925) - 2050376..2050780 (-) 405 WP_249663278.1 hypothetical protein -
  VNN27_RS09930 (VNN27_09930) - 2050792..2051667 (-) 876 WP_346349980.1 carbohydrate binding domain-containing protein -
  VNN27_RS09935 (VNN27_09935) - 2051671..2052615 (-) 945 WP_346349981.1 BppU family phage baseplate upper protein -
  VNN27_RS09940 (VNN27_09940) - 2052631..2054331 (-) 1701 WP_346350230.1 phage tail tip lysozyme -
  VNN27_RS09945 (VNN27_09945) - 2054431..2055465 (-) 1035 Protein_1911 phage tail spike protein -
  VNN27_RS09950 (VNN27_09950) - 2055465..2056226 (-) 762 WP_346349982.1 distal tail protein Dit -
  VNN27_RS09955 (VNN27_09955) - 2056235..2058586 (-) 2352 WP_346349983.1 tape measure protein -
  VNN27_RS09960 (VNN27_09960) - 2058600..2058860 (-) 261 WP_279364706.1 hypothetical protein -
  VNN27_RS09965 (VNN27_09965) - 2058947..2059294 (-) 348 WP_346349984.1 tail assembly chaperone -
  VNN27_RS09970 (VNN27_09970) - 2059343..2059873 (-) 531 WP_274978398.1 phage major tail protein, TP901-1 family -
  VNN27_RS09975 (VNN27_09975) - 2059883..2060272 (-) 390 WP_346349985.1 phage capsid protein -
  VNN27_RS09980 (VNN27_09980) - 2060269..2060595 (-) 327 WP_346349986.1 HK97-gp10 family putative phage morphogenesis protein -
  VNN27_RS09985 (VNN27_09985) - 2060592..2060903 (-) 312 WP_276414278.1 hypothetical protein -
  VNN27_RS09990 (VNN27_09990) - 2060900..2061232 (-) 333 WP_346349987.1 phage head-tail connector protein -
  VNN27_RS09995 (VNN27_09995) - 2061243..2061482 (-) 240 WP_346349988.1 Ig-like domain-containing protein -
  VNN27_RS10000 (VNN27_10000) - 2061500..2062324 (-) 825 WP_346349989.1 N4-gp56 family major capsid protein -
  VNN27_RS10005 (VNN27_10005) - 2062324..2063004 (-) 681 WP_346349990.1 DUF4355 domain-containing protein -
  VNN27_RS10010 (VNN27_10010) - 2063134..2064165 (-) 1032 WP_346349991.1 minor capsid protein -
  VNN27_RS10015 (VNN27_10015) - 2064169..2065512 (-) 1344 WP_346349992.1 phage portal protein -
  VNN27_RS10020 (VNN27_10020) - 2065513..2066733 (-) 1221 WP_346349993.1 PBSX family phage terminase large subunit -
  VNN27_RS10025 (VNN27_10025) - 2066726..2067235 (-) 510 WP_346349994.1 terminase small subunit -
  VNN27_RS10030 (VNN27_10030) - 2067369..2068055 (-) 687 WP_346349995.1 CHAP domain-containing protein -
  VNN27_RS10035 (VNN27_10035) - 2068204..2069499 (-) 1296 WP_346349996.1 hypothetical protein -
  VNN27_RS10040 (VNN27_10040) - 2069526..2070620 (-) 1095 WP_346349997.1 hypothetical protein -
  VNN27_RS10045 (VNN27_10045) - 2071026..2071427 (-) 402 WP_249663255.1 DUF722 domain-containing protein -
  VNN27_RS10050 (VNN27_10050) - 2071618..2072016 (+) 399 WP_346349998.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  VNN27_RS10055 (VNN27_10055) - 2072082..2072363 (-) 282 WP_346349999.1 hypothetical protein -
  VNN27_RS10060 (VNN27_10060) - 2072471..2072875 (-) 405 WP_346350000.1 RusA family crossover junction endodeoxyribonuclease -
  VNN27_RS10065 (VNN27_10065) - 2072872..2073156 (-) 285 WP_276421239.1 hypothetical protein -
  VNN27_RS10070 (VNN27_10070) - 2073140..2073928 (-) 789 WP_346350001.1 helix-turn-helix domain-containing protein -
  VNN27_RS10075 (VNN27_10075) - 2073931..2074095 (-) 165 WP_249663249.1 hypothetical protein -
  VNN27_RS10080 (VNN27_10080) - 2074095..2074325 (-) 231 WP_346350002.1 helix-turn-helix domain-containing protein -
  VNN27_RS10085 (VNN27_10085) ssb 2074425..2074841 (-) 417 WP_346350003.1 single-stranded DNA-binding protein Machinery gene
  VNN27_RS10090 (VNN27_10090) - 2074831..2075511 (-) 681 WP_346350004.1 DUF1071 domain-containing protein -
  VNN27_RS10095 (VNN27_10095) - 2075523..2075921 (-) 399 WP_346350005.1 hypothetical protein -
  VNN27_RS10100 (VNN27_10100) - 2076087..2076650 (+) 564 WP_346350006.1 hypothetical protein -
  VNN27_RS10105 (VNN27_10105) - 2076740..2076889 (-) 150 WP_019292873.1 hypothetical protein -
  VNN27_RS10110 (VNN27_10110) - 2076906..2077157 (-) 252 WP_270745004.1 hypothetical protein -
  VNN27_RS10115 (VNN27_10115) - 2077170..2077400 (-) 231 WP_153924777.1 hypothetical protein -
  VNN27_RS10120 (VNN27_10120) - 2077556..2078011 (+) 456 WP_346350007.1 alkylphosphonate ABC transporter permease -
  VNN27_RS10125 (VNN27_10125) - 2078407..2078751 (+) 345 WP_019292507.1 XRE family transcriptional regulator -
  VNN27_RS10130 (VNN27_10130) - 2078763..2079353 (+) 591 WP_346350008.1 hypothetical protein -
  VNN27_RS10135 (VNN27_10135) - 2079401..2080468 (+) 1068 WP_346350009.1 hypothetical protein -
  VNN27_RS10140 (VNN27_10140) - 2080511..2080885 (+) 375 WP_014572153.1 PH domain-containing protein -
  VNN27_RS10145 (VNN27_10145) - 2081009..2082133 (+) 1125 WP_346350010.1 site-specific integrase -

Sequence


Protein


Download         Length: 138 a.a.        Molecular weight: 15586.51 Da        Isoelectric Point: 6.3836

>NTDB_id=918514 VNN27_RS10085 WP_346350003.1 2074425..2074841(-) (ssb) [Lactococcus petauri strain R22-25 IC35]
MINNVVLVGRIVRDPELRYTSQNTAVATFTLAVNRRFKNAQGEREADFINCVIWRQPAENLANWAKKGTLVGITGSIQVR
NYENKEGQRVYVTEVLADNFQMLESNSNKTEKGKTKSNQDKDLFAGSPMEVSDDDLPF

Nucleotide


Download         Length: 417 bp        

>NTDB_id=918514 VNN27_RS10085 WP_346350003.1 2074425..2074841(-) (ssb) [Lactococcus petauri strain R22-25 IC35]
ATGATAAATAATGTTGTGTTGGTTGGACGTATTGTCCGTGATCCAGAATTAAGATATACGTCACAGAATACTGCAGTAGC
TACTTTCACTTTAGCAGTAAATCGCCGTTTTAAAAATGCTCAGGGTGAACGAGAAGCAGATTTTATAAACTGTGTTATCT
GGAGACAGCCCGCTGAAAACTTAGCAAATTGGGCAAAAAAAGGTACTTTAGTTGGTATTACTGGAAGTATTCAAGTGAGA
AATTATGAGAACAAGGAAGGTCAACGTGTTTATGTAACAGAAGTATTGGCTGATAACTTTCAAATGCTAGAAAGTAATTC
AAATAAAACAGAAAAAGGGAAAACAAAATCTAATCAAGATAAAGATCTTTTTGCAGGTTCACCAATGGAAGTCTCAGATG
ATGATTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

52.353

100

0.645

  ssbA Bacillus subtilis subsp. subtilis str. 168

50

100

0.623

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.577

75.362

0.457

  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.928

100

0.449

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.478

100

0.435

  ssbB/cilA Streptococcus mitis NCTC 12261

42.754

100

0.428

  ssbB/cilA Streptococcus pneumoniae Rx1

42.754

100

0.428

  ssbB/cilA Streptococcus pneumoniae D39

42.754

100

0.428

  ssbB/cilA Streptococcus pneumoniae R6

42.754

100

0.428

  ssbB/cilA Streptococcus mitis SK321

42.754

100

0.428

  ssbA Streptococcus mutans UA159

40.58

100

0.406