Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   SO574_RS12290 Genome accession   NZ_CP140102
Coordinates   2707261..2708940 (-) Length   559 a.a.
NCBI ID   WP_409202761.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain VA-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2702261..2713940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SO574_RS12270 (SO574_12270) - 2702950..2703789 (-) 840 WP_221068214.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  SO574_RS23675 - 2703859..2704248 (-) 390 Protein_2342 SPOR domain-containing protein -
  SO574_RS12275 (SO574_12275) - 2704330..2705373 (-) 1044 Protein_2343 ATP-binding protein -
  SO574_RS12280 (SO574_12280) aroB 2705425..2706525 (-) 1101 WP_211915860.1 3-dehydroquinate synthase -
  SO574_RS12285 (SO574_12285) aroK 2706554..2707072 (-) 519 WP_099080818.1 shikimate kinase AroK -
  SO574_RS12290 (SO574_12290) pilQ 2707261..2708940 (-) 1680 WP_409202761.1 type IV pilus secretin PilQ Machinery gene
  SO574_RS12295 (SO574_12295) pilP 2709000..2709509 (-) 510 WP_322803224.1 pilus assembly protein PilP Machinery gene
  SO574_RS12300 (SO574_12300) pilO 2709499..2710092 (-) 594 WP_322803225.1 type 4a pilus biogenesis protein PilO Machinery gene
  SO574_RS12305 (SO574_12305) pilN 2710085..2710660 (-) 576 WP_322804187.1 PilN domain-containing protein Machinery gene
  SO574_RS12310 (SO574_12310) pilM 2710644..2711657 (-) 1014 WP_221068220.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61433.18 Da        Isoelectric Point: 4.6455

>NTDB_id=912532 SO574_RS12290 WP_409202761.1 2707261..2708940(-) (pilQ) [Vibrio alfacsensis strain VA-1]
MVVGFFSVWLFVFLALEGRAKASPPNELKSIDFRADSNTEAVIMIELTSSSVVVDIQREKEGLSIELLNTSLSDNQVHVL
DVRDFSTVIKQIDVSRHHSATRLLAYSNDEYRYDYRLLGRQLEVTVVKEKSTKQTQNQNILKNKGRLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVSGNLTLRLDDVPWPQVLDIILQVKGLDKRVDGNVILIAPLDELDLREKQRLEKQKLELEIGE
LSSEIIKVNFAKASDVAEMIYGEGNISMLSERGSMTVDERTNSLLVRELPENIDVIREIIEALDIPVKQVQIEARIVTIN
EGNLDELGVRWGFTSINGSSTVGGSIENNLATLGLYDGGVGIDDFLNVNLAASNPSATSLAFQVAKLGAGTLLDLELSAL
QQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGQVVK
TGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSLSNAVDKVPLLGDLPLLGALFRRSYEKVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=912532 SO574_RS12290 WP_409202761.1 2707261..2708940(-) (pilQ) [Vibrio alfacsensis strain VA-1]
ATGGTGGTTGGTTTTTTCTCTGTGTGGCTATTTGTGTTTTTGGCATTAGAGGGGAGAGCAAAGGCGTCACCACCGAATGA
ATTAAAATCGATAGACTTCCGTGCTGATAGCAATACAGAGGCGGTGATCATGATTGAATTAACATCATCGTCTGTCGTTG
TTGATATTCAACGTGAAAAAGAAGGGCTAAGCATTGAACTGCTTAATACTTCGCTCAGTGATAACCAAGTGCACGTCTTG
GATGTAAGGGATTTTTCTACGGTCATTAAGCAAATTGACGTTTCTCGGCATCATTCAGCCACGCGTTTGTTGGCCTATTC
GAATGATGAGTATCGCTATGACTATCGTTTGTTGGGACGGCAATTGGAAGTGACGGTGGTGAAAGAGAAATCAACTAAGC
AAACACAAAACCAGAACATTTTGAAGAATAAAGGGCGTTTAATCTCTATCAATTTTCAAGATATTCCTGTGCGAAATGTA
TTGCAGTTAATCGCTGATTATAATGATTTCAACCTCGTCGTGTCGGATTCAGTGTCGGGTAATCTGACGTTACGCTTAGA
TGACGTACCTTGGCCGCAAGTGTTGGATATCATTTTGCAAGTAAAAGGATTGGATAAGCGGGTTGATGGCAATGTCATTT
TGATTGCCCCTTTAGATGAACTGGATCTGCGAGAGAAGCAACGTCTTGAAAAGCAAAAGCTTGAACTGGAGATAGGTGAG
CTTTCCTCTGAGATTATTAAAGTGAATTTTGCAAAAGCCTCGGACGTAGCAGAAATGATCTATGGTGAGGGTAATATCAG
CATGCTTTCTGAGCGAGGAAGTATGACCGTTGATGAGCGCACGAATTCATTGTTGGTGCGAGAATTACCAGAGAACATTG
ACGTTATTCGCGAAATTATAGAAGCGTTAGACATTCCCGTGAAACAAGTACAGATTGAAGCTCGCATTGTCACCATTAAT
GAGGGGAACTTGGATGAGCTAGGGGTTCGTTGGGGCTTTACAAGTATAAATGGCAGTTCAACGGTTGGAGGCTCGATTGA
AAATAATCTCGCCACACTCGGTTTATATGATGGTGGTGTTGGTATTGATGATTTTCTTAACGTTAACCTGGCGGCGAGCA
ACCCAAGTGCTACTAGCCTTGCTTTTCAAGTGGCAAAGTTAGGGGCAGGGACTTTGCTTGATTTAGAACTGTCTGCGTTG
CAGCAGGAGTCAAAAGCAGAAATTATCTCGAGTCCACGGTTGATCACCACCAATAAGAAACCCGCCTATATTGAGCAGGG
AACGGAAATCCCGTATTTAGAATCGTCATCAAGTGGGGCGACGTCGGTGACATTTAAAAAAGCAGTATTGAGTTTAAAAG
TTACGCCTCAGATAACGCCAGATAATCGCTTGGTGTTGGATTTGAGTGTAACTCAAGACCGGCCTGGTCAAGTGGTGAAA
ACAGGCACGGGAGAAGCGGTTGCAATTGATACTCAGAGAATTGGCACTCAAGTTTTGGTCAATAATGGTGAAACGGTGGT
ATTGGGTGGTATTTTCCAACATAGCCTCAGTAATGCCGTTGACAAAGTCCCTTTACTTGGTGATTTACCCTTGCTTGGCG
CGCTTTTTCGCCGAAGTTATGAAAAAGTAGGCAAAAGTGAGTTGTTGATTTTCGTGACGCCAAAGGTCGTAATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)

Secretin and TonB N terminus short domain (STN)

(170-217)

Bacterial type II and III secretion system protein (Secretin)

(399-557)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

81.768

97.138

0.794
  pilQ Vibrio cholerae strain A1552

70.662

100

0.707
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

70.662

100

0.707
  pilQ Pseudomonas aeruginosa PAK

40.463

100

0.406
  pilQ Legionella pneumophila strain Lp02

41.296

96.601

0.399
  pilQ Legionella pneumophila strain ERS1305867

41.296

96.601

0.399
  comQ Acinetobacter baylyi ADP1

38.272

100

0.388
  pilQ Acinetobacter baumannii D1279779

36.426

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.426

100

0.379