Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   SO574_RS12310 Genome accession   NZ_CP140102
Coordinates   2710644..2711657 (-) Length   337 a.a.
NCBI ID   WP_221068220.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain VA-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2705644..2716657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SO574_RS12285 (SO574_12285) aroK 2706554..2707072 (-) 519 WP_099080818.1 shikimate kinase AroK -
  SO574_RS12290 (SO574_12290) pilQ 2707261..2708940 (-) 1680 WP_409202761.1 type IV pilus secretin PilQ Machinery gene
  SO574_RS12295 (SO574_12295) pilP 2709000..2709509 (-) 510 WP_322803224.1 pilus assembly protein PilP Machinery gene
  SO574_RS12300 (SO574_12300) pilO 2709499..2710092 (-) 594 WP_322803225.1 type 4a pilus biogenesis protein PilO Machinery gene
  SO574_RS12305 (SO574_12305) pilN 2710085..2710660 (-) 576 WP_322804187.1 PilN domain-containing protein Machinery gene
  SO574_RS12310 (SO574_12310) pilM 2710644..2711657 (-) 1014 WP_221068220.1 type IV pilus biogenesis protein PilM Machinery gene
  SO574_RS12315 (SO574_12315) - 2711799..2714363 (+) 2565 WP_322803226.1 PBP1A family penicillin-binding protein -
  SO574_RS12320 (SO574_12320) oxyR 2714435..2715343 (-) 909 WP_128810015.1 DNA-binding transcriptional regulator OxyR -
  SO574_RS12325 (SO574_12325) - 2715516..2716244 (+) 729 WP_128810014.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 37664.06 Da        Isoelectric Point: 7.0389

>NTDB_id=912536 SO574_RS12310 WP_221068220.1 2710644..2711657(-) (pilM) [Vibrio alfacsensis strain VA-1]
MDKLVVTGIDIGHNSLKAVVLKPSGRHYAVLDYKEIILKECIVAENNTINHQEIVKTLKEIKKALPFGAKRVAISVPENA
VISKKLQIDPGLDSSERDYAVMQVFAHQSPFPLQELSLDFVLDDVKNKTDGLEHYQVFATRKDVVDSRVDALQQSGLKPV
LVEVASQSLGHMWKLATEHFPEKSTYCLLNVGNVKSSFTMFTEQGELFHKEFSSSSWSEKTPSVYQKGACLQVDESHQEQ
ALIERVQRQLQLYASFNGSLNITGIWITGDGFVTPQLKRALSSQLSMECETLNPFELFTLKASLHKHKAVQSHQFTAAAG
LAIRGIHWLEHGNVVSH

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=912536 SO574_RS12310 WP_221068220.1 2710644..2711657(-) (pilM) [Vibrio alfacsensis strain VA-1]
ATGGACAAACTCGTCGTTACTGGCATCGACATAGGCCATAACAGTCTCAAAGCCGTGGTACTTAAGCCTAGTGGTCGCCA
TTACGCGGTACTGGACTACAAAGAAATAATATTGAAAGAATGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACACTTAAAGAGATAAAAAAAGCACTGCCTTTCGGGGCTAAACGTGTCGCGATTTCGGTGCCAGAAAATGCC
GTGATCAGCAAAAAACTGCAAATAGATCCAGGGTTAGATAGCAGTGAACGAGACTATGCGGTCATGCAAGTTTTCGCTCA
TCAATCACCTTTCCCCTTACAAGAGCTTAGTTTAGATTTTGTTTTAGATGATGTGAAGAATAAAACGGATGGACTTGAAC
ATTATCAAGTATTTGCTACTCGTAAGGACGTTGTTGATTCTCGAGTCGATGCTTTACAACAATCAGGGTTAAAGCCTGTG
TTAGTAGAGGTGGCATCACAAAGCTTAGGTCATATGTGGAAGCTCGCGACGGAGCATTTTCCAGAGAAAAGTACCTACTG
CTTGCTCAATGTCGGCAACGTTAAATCATCGTTCACAATGTTTACTGAACAGGGTGAATTATTTCACAAAGAGTTCTCAT
CAAGTTCATGGTCTGAAAAAACGCCATCAGTATATCAGAAAGGTGCTTGTTTACAGGTGGATGAATCACACCAAGAGCAA
GCTCTGATTGAGCGAGTTCAGCGTCAACTGCAGCTTTATGCTTCTTTCAATGGTTCTCTCAATATTACCGGTATCTGGAT
TACAGGTGACGGTTTTGTCACACCCCAATTAAAGAGAGCGTTATCGAGTCAATTGAGCATGGAATGTGAAACACTCAACC
CGTTCGAGTTGTTTACATTGAAGGCGTCACTGCATAAGCATAAGGCTGTTCAATCGCACCAGTTTACTGCTGCTGCGGGC
CTCGCGATTCGAGGTATTCATTGGTTGGAGCATGGCAATGTTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-211)

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

63.235

100

0.638
  pilM Vibrio cholerae strain A1552

41.765

100

0.421