Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SOI79_RS09355 Genome accession   NZ_CP139266
Coordinates   1958231..1958665 (+) Length   144 a.a.
NCBI ID   WP_014207821.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HUM14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1953231..1963665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SOI79_RS09335 (SOI79_09335) - 1954612..1955499 (+) 888 WP_014207824.1 metal-dependent hydrolase -
  SOI79_RS09340 (SOI79_09340) - 1955630..1956220 (+) 591 WP_002117090.1 LemA family protein -
  SOI79_RS09345 (SOI79_09345) - 1956245..1957327 (+) 1083 WP_068976280.1 TPM domain-containing protein -
  SOI79_RS09350 (SOI79_09350) - 1957321..1957881 (+) 561 WP_068976281.1 TPM domain-containing protein -
  SOI79_RS09355 (SOI79_09355) pilA 1958231..1958665 (+) 435 WP_014207821.1 pilin Machinery gene
  SOI79_RS09360 (SOI79_09360) - 1958724..1960037 (+) 1314 WP_032004878.1 O-antigen ligase family protein -
  SOI79_RS09365 (SOI79_09365) - 1960143..1961771 (+) 1629 WP_017481721.1 PglL family O-oligosaccharyltransferase -
  SOI79_RS09370 (SOI79_09370) bfr 1961814..1962278 (-) 465 WP_005067113.1 heteropolymeric bacterioferritin subunit Bfr -
  SOI79_RS09375 (SOI79_09375) bfd 1962522..1962716 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  SOI79_RS09380 (SOI79_09380) - 1962819..1962946 (+) 128 Protein_1814 hypothetical protein -
  SOI79_RS09385 (SOI79_09385) - 1962936..1963319 (-) 384 WP_002116996.1 RidA family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14802.90 Da        Isoelectric Point: 8.1106

>NTDB_id=908530 SOI79_RS09355 WP_014207821.1 1958231..1958665(+) (pilA) [Acinetobacter pittii strain HUM14]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVSTGLADITAGKTNAETKLAEGLTAALTDVTTLGLQASTNAC
AVTANIGTNGASNITCTLKGTSQINGKKIEWIRDADNATNGTTGAWRCKTDVAENLRPKSCGAS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=908530 SOI79_RS09355 WP_014207821.1 1958231..1958665(+) (pilA) [Acinetobacter pittii strain HUM14]
ATGAATGCACAAAAAGGTTTTACATTAATTGAGCTTATGATCGTGGTTGCCATTATTGGTATTTTGGCAGCAATTGCGAT
TCCTGCATATCAAAACTATATTGCTAAGTCACAAGTAAGTACTGGCTTGGCTGATATTACAGCTGGTAAAACAAATGCAG
AAACTAAATTGGCAGAAGGTTTAACCGCTGCATTAACCGATGTAACAACTTTAGGCTTACAAGCATCTACGAATGCTTGT
GCTGTTACAGCGAATATTGGAACTAATGGTGCAAGTAATATTACTTGTACACTGAAGGGTACATCACAAATTAATGGTAA
GAAAATTGAATGGATCCGTGATGCGGATAATGCTACAAATGGTACGACAGGTGCTTGGCGCTGTAAGACAGATGTAGCTG
AAAACTTACGTCCTAAATCATGTGGTGCTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.381

100

0.535
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

43.709

100

0.458
  pilA Vibrio cholerae strain A1552

43.709

100

0.458
  pilA Vibrio cholerae C6706

43.709

100

0.458
  pilA Pseudomonas aeruginosa PAK

42.208

100

0.451
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.07

98.611

0.444
  comP Acinetobacter baylyi ADP1

41.447

100

0.437
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.972

97.917

0.431
  pilA Vibrio campbellii strain DS40M4

40.278

100

0.403
  pilA Vibrio parahaemolyticus RIMD 2210633

44

86.806

0.382