Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R2E40_RS14220 Genome accession   NZ_CP137631
Coordinates   3089721..3090860 (+) Length   379 a.a.
NCBI ID   WP_318163673.1    Uniprot ID   -
Organism   Aeromonas sp. CD     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3084721..3095860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2E40_RS14190 (R2E40_14190) - 3085028..3086092 (+) 1065 WP_318163669.1 3-deoxy-7-phosphoheptulonate synthase -
  R2E40_RS14195 (R2E40_14195) aac(6') 3086099..3086557 (+) 459 WP_318163670.1 aminoglycoside 6'-N-acetyltransferase -
  R2E40_RS14200 (R2E40_14200) - 3086579..3087364 (-) 786 WP_282467605.1 ATP-binding cassette domain-containing protein -
  R2E40_RS14205 (R2E40_14205) btuC 3087351..3088358 (-) 1008 WP_318163671.1 vitamin B12 ABC transporter permease BtuC -
  R2E40_RS14210 (R2E40_14210) - 3088459..3089190 (-) 732 WP_318163672.1 transporter substrate-binding domain-containing protein -
  R2E40_RS14215 (R2E40_14215) - 3089304..3089660 (+) 357 WP_005302630.1 DMT family protein -
  R2E40_RS14220 (R2E40_14220) pilU 3089721..3090860 (+) 1140 WP_318163673.1 type IVa pilus ATPase TapW Machinery gene
  R2E40_RS14225 (R2E40_14225) glgC 3091144..3092415 (+) 1272 WP_024946380.1 glucose-1-phosphate adenylyltransferase -
  R2E40_RS14230 (R2E40_14230) - 3092551..3093030 (+) 480 WP_167385334.1 WbuC family cupin fold metalloprotein -
  R2E40_RS14235 (R2E40_14235) - 3093146..3093388 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  R2E40_RS14240 (R2E40_14240) feoB 3093385..3095658 (-) 2274 WP_318163674.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42236.27 Da        Isoelectric Point: 6.4290

>NTDB_id=899553 R2E40_RS14220 WP_318163673.1 3089721..3090860(+) (pilU) [Aeromonas sp. CD]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGAIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=899553 R2E40_RS14220 WP_318163673.1 3089721..3090860(+) (pilU) [Aeromonas sp. CD]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAAACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCCCCCGTACTGCTCGACACCATCATGGAAAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACCTCGCTCGCGGCCCTCATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCATCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
AGCCCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATTCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGCCCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCAGCGACGGTGGTCGCCGGGCAGCGGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGCGGCGAATTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGCGCGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.36

93.14

0.385
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

87.863

0.361
  pilT Vibrio cholerae strain A1552

41.141

87.863

0.361