Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4700_RS04550 Genome accession   NZ_CP137112
Coordinates   870596..871537 (-) Length   313 a.a.
NCBI ID   WP_000249573.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 02H2025     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 865596..876537
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4700_RS04510 comYH 866082..867035 (-) 954 WP_277770963.1 class I SAM-dependent methyltransferase Machinery gene
  R4700_RS04515 - 867096..867690 (-) 595 Protein_862 class I SAM-dependent methyltransferase -
  R4700_RS04520 comGG/cglG 867827..868240 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4700_RS04525 comGF/cglF 868218..868679 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4700_RS04530 comGE/cglE 868642..868944 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4700_RS04535 comGD/cglD 868907..869311 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4700_RS04540 comGC/cglC 869304..869630 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  R4700_RS04545 comGB/cglB 869632..870648 (-) 1017 WP_078132591.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4700_RS04550 comGA/cglA/cilD 870596..871537 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  R4700_RS04555 - 871613..871978 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4700_RS04560 - 872129..873187 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4700_RS04565 nagA 873350..874501 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4700_RS04570 - 874654..876471 (-) 1818 WP_001220838.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35564.49 Da        Isoelectric Point: 6.0083

>NTDB_id=896030 R4700_RS04550 WP_000249573.1 870596..871537(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 02H2025]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=896030 R4700_RS04550 WP_000249573.1 870596..871537(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 02H2025]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTTCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAACAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGTTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361