Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4700_RS04545 Genome accession   NZ_CP137112
Coordinates   869632..870648 (-) Length   338 a.a.
NCBI ID   WP_078132591.1    Uniprot ID   A0A0T8LTS4
Organism   Streptococcus pneumoniae strain 02H2025     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 864632..875648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4700_RS04500 - 864709..864840 (-) 132 WP_000768904.1 hypothetical protein -
  R4700_RS04505 - 864841..866031 (-) 1191 WP_277770962.1 acetate kinase -
  R4700_RS04510 comYH 866082..867035 (-) 954 WP_277770963.1 class I SAM-dependent methyltransferase Machinery gene
  R4700_RS04515 - 867096..867690 (-) 595 Protein_862 class I SAM-dependent methyltransferase -
  R4700_RS04520 comGG/cglG 867827..868240 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4700_RS04525 comGF/cglF 868218..868679 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4700_RS04530 comGE/cglE 868642..868944 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4700_RS04535 comGD/cglD 868907..869311 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4700_RS04540 comGC/cglC 869304..869630 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  R4700_RS04545 comGB/cglB 869632..870648 (-) 1017 WP_078132591.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4700_RS04550 comGA/cglA/cilD 870596..871537 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  R4700_RS04555 - 871613..871978 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4700_RS04560 - 872129..873187 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4700_RS04565 nagA 873350..874501 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38518.56 Da        Isoelectric Point: 9.4803

>NTDB_id=896029 R4700_RS04545 WP_078132591.1 869632..870648(-) (comGB/cglB) [Streptococcus pneumoniae strain 02H2025]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMIVFVSVGVLLALTFYKRSSKMHVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=896029 R4700_RS04545 WP_078132591.1 869632..870648(-) (comGB/cglB) [Streptococcus pneumoniae strain 02H2025]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCACAAATCTTT
CTAGGAATGATAGTTTTTGTTTCAGTAGGTGTCCTTTTAGCACTCACTTTTTATAAAAGAAGTTCTAAGATGCACGTCTT
TTCTATCTTAGCGCGCCTTCCTTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T8LTS4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae D39

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae R6

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae TIGR4

97.929

100

0.979
  comGB/cglB Streptococcus mitis NCTC 12261

95.266

100

0.953
  comGB/cglB Streptococcus mitis SK321

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.621

94.379

0.553
  comYB Streptococcus mutans UA159

58.621

94.379

0.553
  comGB Lactococcus lactis subsp. cremoris KW2

50.299

98.817

0.497