Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   R4700_RS04525 Genome accession   NZ_CP137112
Coordinates   868218..868679 (-) Length   153 a.a.
NCBI ID   WP_000250534.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 02H2025     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 863218..873679
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4700_RS04490 - 863522..864346 (-) 825 WP_000727909.1 membrane protein insertase YidC -
  R4700_RS04495 rnpA 864321..864692 (-) 372 WP_000739246.1 ribonuclease P protein component -
  R4700_RS04500 - 864709..864840 (-) 132 WP_000768904.1 hypothetical protein -
  R4700_RS04505 - 864841..866031 (-) 1191 WP_277770962.1 acetate kinase -
  R4700_RS04510 comYH 866082..867035 (-) 954 WP_277770963.1 class I SAM-dependent methyltransferase Machinery gene
  R4700_RS04515 - 867096..867690 (-) 595 Protein_862 class I SAM-dependent methyltransferase -
  R4700_RS04520 comGG/cglG 867827..868240 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4700_RS04525 comGF/cglF 868218..868679 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4700_RS04530 comGE/cglE 868642..868944 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4700_RS04535 comGD/cglD 868907..869311 (-) 405 WP_000588024.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4700_RS04540 comGC/cglC 869304..869630 (-) 327 WP_000738630.1 comG operon protein ComGC Machinery gene
  R4700_RS04545 comGB/cglB 869632..870648 (-) 1017 WP_078132591.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4700_RS04550 comGA/cglA/cilD 870596..871537 (-) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  R4700_RS04555 - 871613..871978 (-) 366 WP_000286415.1 DUF1033 family protein -
  R4700_RS04560 - 872129..873187 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17972.67 Da        Isoelectric Point: 9.6092

>NTDB_id=896025 R4700_RS04525 WP_000250534.1 868218..868679(-) (comGF/cglF) [Streptococcus pneumoniae strain 02H2025]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSVRITEDNQLVRFHFQFQKGLEREFIYRVEKEKS

Nucleotide


Download         Length: 462 bp        

>NTDB_id=896025 R4700_RS04525 WP_000250534.1 868218..868679(-) (comGF/cglF) [Streptococcus pneumoniae strain 02H2025]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGATGATTTCCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGACTCAAATCTGTACGGATTACAGAGGACAATCAACTGGTTCGCTTTCATT
TCCAGTTCCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

100

100

1
  comGF/cglF Streptococcus pneumoniae D39

100

100

1
  comGF/cglF Streptococcus pneumoniae R6

100

100

1
  comGF/cglF Streptococcus pneumoniae TIGR4

100

100

1
  comGF/cglF Streptococcus mitis SK321

90.85

100

0.909
  comGF/cglF Streptococcus mitis NCTC 12261

89.542

100

0.895