Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AAHT64_RS07570 Genome accession   NZ_CP155054
Coordinates   1455197..1455607 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain R38     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1450197..1460607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHT64_RS07535 (AAHT64_07535) arsR 1450360..1450677 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  AAHT64_RS07540 (AAHT64_07540) yqcK 1450738..1451178 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  AAHT64_RS07545 (AAHT64_07545) acr3 1451201..1452241 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  AAHT64_RS07550 (AAHT64_07550) arsC 1452253..1452672 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  AAHT64_RS07555 (AAHT64_07555) - 1453027..1453205 (+) 179 Protein_1481 hypothetical protein -
  AAHT64_RS07560 (AAHT64_07560) spoIVCA 1453163..1454623 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  AAHT64_RS07565 (AAHT64_07565) - 1454654..1455001 (-) 348 Protein_1483 sigma-70 family RNA polymerase sigma factor -
  AAHT64_RS07570 (AAHT64_07570) nucA/comI 1455197..1455607 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  AAHT64_RS07575 (AAHT64_07575) yqeB 1455640..1456362 (-) 723 WP_010886572.1 hypothetical protein -
  AAHT64_RS07580 (AAHT64_07580) gnd 1456614..1457507 (+) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  AAHT64_RS07585 (AAHT64_07585) yqeD 1457526..1458152 (-) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  AAHT64_RS07590 (AAHT64_07590) cwlH 1458339..1459091 (+) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  AAHT64_RS07595 (AAHT64_07595) yqeF 1459343..1460074 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  AAHT64_RS07600 (AAHT64_07600) - 1460380..1460520 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=895503 AAHT64_RS07570 WP_009967785.1 1455197..1455607(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain R38]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=895503 AAHT64_RS07570 WP_009967785.1 1455197..1455607(+) (nucA/comI) [Bacillus subtilis subsp. subtilis strain R38]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529