Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   M9Y83_RS27170 Genome accession   NZ_CP155048
Coordinates   5715383..5715847 (-) Length   154 a.a.
NCBI ID   WP_250180515.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain CCBH26428     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5710383..5720847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M9Y83_RS27140 (M9Y83_0027140) fliQ 5711069..5711338 (+) 270 WP_023435365.1 flagellar biosynthesis protein FliQ -
  M9Y83_RS27145 (M9Y83_0027145) fliR 5711338..5712120 (+) 783 Protein_5374 flagellar biosynthetic protein FliR -
  M9Y83_RS27150 (M9Y83_0027150) - 5712123..5712382 (+) 260 Protein_5375 EscU/YscU/HrcU family type III secretion system export apparatus switch protein -
  M9Y83_RS27155 (M9Y83_0027155) - 5713411..5713653 (+) 243 WP_023082027.1 hypothetical protein -
  M9Y83_RS27165 (M9Y83_0027165) - 5713912..5715225 (-) 1314 WP_234426410.1 O-antigen ligase family protein -
  M9Y83_RS27170 (M9Y83_0027170) pilA/pilAI 5715383..5715847 (-) 465 WP_250180515.1 pilin Machinery gene
  M9Y83_RS27175 (M9Y83_0027175) pilB 5716078..5717778 (+) 1701 WP_023835879.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M9Y83_RS27180 (M9Y83_0027180) pilC 5717782..5718999 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  M9Y83_RS27185 (M9Y83_0027185) pilD 5719000..5719872 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M9Y83_RS27190 (M9Y83_0027190) coaE 5719869..5720480 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  M9Y83_RS27195 (M9Y83_0027195) yacG 5720477..5720677 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16219.59 Da        Isoelectric Point: 9.3978

>NTDB_id=895423 M9Y83_RS27170 WP_250180515.1 5715383..5715847(-) (pilA/pilAI) [Pseudomonas aeruginosa strain CCBH26428]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEISALKTAAESAILEGKKLVSNDSPKNDEYDLGFTS
STLLTGDGKKQIKIDKADTATPEISGTLGRSSGKGIAGAVITVKRDDKGVWTCGITGSPTNWKANYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=895423 M9Y83_RS27170 WP_250180515.1 5715383..5715847(-) (pilA/pilAI) [Pseudomonas aeruginosa strain CCBH26428]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAATCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGCAAGAAGCTCGTTTCCAACGATTCTCCCAAAAACGATGAGTATGATCTTGGCTTTACCAGT
TCTACTCTGCTTACCGGTGACGGTAAGAAGCAGATCAAGATTGACAAAGCTGATACCGCAACTCCGGAGATTTCTGGTAC
CTTGGGCCGCTCTTCTGGTAAAGGTATTGCTGGCGCTGTCATCACTGTCAAGCGTGATGATAAAGGAGTATGGACCTGCG
GCATCACTGGTTCGCCGACCAACTGGAAAGCCAACTACGCTCCGGCCAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.516

100

0.448
  pilA Acinetobacter baumannii strain A118

42.857

100

0.429
  pilA Pseudomonas aeruginosa PAK

41.139

100

0.422
  pilA Ralstonia pseudosolanacearum GMI1000

37.059

100

0.409
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.132

98.701

0.396
  pilA Vibrio cholerae strain A1552

40.132

98.701

0.396
  pilA Vibrio cholerae C6706

40.132

98.701

0.396
  pilA2 Legionella pneumophila strain ERS1305867

39.189

96.104

0.377
  pilA2 Legionella pneumophila str. Paris

37.838

96.104

0.364