Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   R4535_RS10685 Genome accession   NZ_CP137091
Coordinates   2384832..2386520 (+) Length   562 a.a.
NCBI ID   WP_076025706.1    Uniprot ID   -
Organism   Vibrio cholerae strain DL4211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2379832..2391520
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS10660 (R4535_10635) fldB 2380355..2380873 (+) 519 WP_000690112.1 flavodoxin FldB -
  R4535_RS10665 (R4535_10640) - 2380920..2382308 (-) 1389 WP_407284727.1 IS4-like element ISVa18 family transposase -
  R4535_RS10670 (R4535_10645) ampD 2382445..2382990 (-) 546 WP_076025708.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  R4535_RS10675 (R4535_10650) nadC 2383286..2384176 (+) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R4535_RS10680 (R4535_10655) - 2384421..2384825 (+) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  R4535_RS10685 (R4535_10660) pilB 2384832..2386520 (+) 1689 WP_076025706.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R4535_RS10690 (R4535_10665) pilC 2386563..2387789 (+) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  R4535_RS10695 (R4535_10670) pilD 2387847..2388722 (+) 876 WP_076025705.1 A24 family peptidase Machinery gene
  R4535_RS10700 (R4535_10675) coaE 2388719..2389327 (+) 609 WP_175597578.1 dephospho-CoA kinase -
  R4535_RS10705 (R4535_10680) zapD 2389359..2390099 (+) 741 WP_000207194.1 cell division protein ZapD -
  R4535_RS10710 (R4535_10685) yacG 2390257..2390454 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62044.10 Da        Isoelectric Point: 6.0856

>NTDB_id=894965 R4535_RS10685 WP_076025706.1 2384832..2386520(+) (pilB) [Vibrio cholerae strain DL4211]
MLTNLVAILRQAELISATQEQAVITQVSASGTSVPEALLGLGIFHAQELTEQLSHIFGLPETDLSRYDYANLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDLRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=894965 R4535_RS10685 WP_076025706.1 2384832..2386520(+) (pilB) [Vibrio cholerae strain DL4211]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAGTTAATCAGCGCAACGCAAGAACAAGCGGTGATTACACAGGT
TAGCGCTTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACGCCCAAGAGCTGACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCAACTTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAGCAAGGCAATTTACTGCTGCTCGCCGTCTCTGACCCGACCTTACT
GCAAGCTGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCGCTAGCCGATCACCGCGCACTGCAAGCGG
CGATTCGCCGTTTGTATGGCAGATCTATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCAAT
CTGGTTAAAGTCAGTGATGACGAACTGCAATCGATCGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCTGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGTTTGCCTCAAGACGGGCGAATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATCTGCGTGTATCCACGCTTCCTACGTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACCGGCCCAACCGGCAGCGGCAAAACCGTTTCACTCTATACTGGGCTGCGCATTCTTAACACGTCACAGATCAA
TATCTCCACCGCGGAAGATCCGGTGGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCAACACTGCACACCAACTCGGCCGCTGAAACCGT
GATTCGCTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCTTGTGTAAACATTGCAAAATCGCGGTGCGCCCTTCTGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCCAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

99.288

100

0.993
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.488

100

0.735
  pilB Legionella pneumophila strain ERS1305867

51.81

93.416

0.484
  pilB Acinetobacter baylyi ADP1

50.484

91.993

0.464
  pilB Acinetobacter baumannii D1279779

50.29

91.993

0.463
  pilF Neisseria gonorrhoeae MS11

45.552

100

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

37

100

0.395
  pilF Thermus thermophilus HB27

40.296

96.263

0.388
  pilB Haemophilus influenzae 86-028NP

39.658

93.772

0.372
  pilB Haemophilus influenzae Rd KW20

39.279

93.772

0.368