Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   R4535_RS10695 Genome accession   NZ_CP137091
Coordinates   2387847..2388722 (+) Length   291 a.a.
NCBI ID   WP_076025705.1    Uniprot ID   -
Organism   Vibrio cholerae strain DL4211     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2382847..2393722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4535_RS10675 (R4535_10650) nadC 2383286..2384176 (+) 891 WP_000665278.1 carboxylating nicotinate-nucleotide diphosphorylase -
  R4535_RS10680 (R4535_10655) - 2384421..2384825 (+) 405 WP_057551631.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  R4535_RS10685 (R4535_10660) pilB 2384832..2386520 (+) 1689 WP_076025706.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R4535_RS10690 (R4535_10665) pilC 2386563..2387789 (+) 1227 WP_000648510.1 type II secretion system F family protein Machinery gene
  R4535_RS10695 (R4535_10670) pilD 2387847..2388722 (+) 876 WP_076025705.1 A24 family peptidase Machinery gene
  R4535_RS10700 (R4535_10675) coaE 2388719..2389327 (+) 609 WP_175597578.1 dephospho-CoA kinase -
  R4535_RS10705 (R4535_10680) zapD 2389359..2390099 (+) 741 WP_000207194.1 cell division protein ZapD -
  R4535_RS10710 (R4535_10685) yacG 2390257..2390454 (+) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  R4535_RS10715 (R4535_10690) parC 2390503..2392788 (-) 2286 WP_207807156.1 DNA topoisomerase IV subunit A -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32479.72 Da        Isoelectric Point: 7.1618

>NTDB_id=894967 R4535_RS10695 WP_076025705.1 2387847..2388722(+) (pilD) [Vibrio cholerae strain DL4211]
MELFYFYPWLFPVLATLFGLIVGSFLNVVIYRLPKIMEREWRAECAASFPEYGITPPEGKLTLSLPRSTCPHCQTPIRVI
DNIPVVSWLALRGQCSHCKAPISARYPLIELLTALMSLVIATHFPFGVFAVALLFFSYVLITATFIDFDTLLLPDQLTLP
LLWGGIALALLGFSPVSLSDAVIGAMAGYLSLWSIYWLFKLLTGKEGMGYGDFKLLAALGTWLGWQQLPIIVLLSSVVGV
IFGLIQLRQQKKGIDMAFPFGPYLAIAGWFALLWGDKVIDWYFTTWVGQPL

Nucleotide


Download         Length: 876 bp        

>NTDB_id=894967 R4535_RS10695 WP_076025705.1 2387847..2388722(+) (pilD) [Vibrio cholerae strain DL4211]
ATGGAACTGTTTTACTTCTACCCTTGGCTGTTTCCGGTGCTCGCCACGCTGTTTGGCTTGATTGTCGGCAGCTTTCTCAA
TGTGGTGATCTATCGCTTACCCAAAATCATGGAGCGTGAATGGCGCGCGGAATGTGCGGCGAGTTTTCCTGAATATGGCA
TCACGCCACCAGAGGGTAAACTGACCCTCAGTTTGCCGCGTTCCACTTGCCCACACTGCCAAACGCCGATTCGCGTCATT
GATAATATTCCGGTAGTCAGTTGGCTGGCGCTGCGCGGTCAATGCTCACATTGTAAAGCGCCGATCAGCGCTCGTTATCC
CTTGATTGAGCTACTCACTGCCCTCATGAGCTTAGTGATTGCTACTCACTTTCCGTTCGGGGTGTTTGCCGTGGCGCTGC
TGTTTTTCAGCTATGTATTGATTACGGCGACCTTCATCGACTTCGATACCCTGTTGCTGCCCGACCAATTAACGCTACCT
TTACTGTGGGGCGGTATTGCACTGGCACTGCTTGGTTTTTCTCCGGTTTCACTCAGTGATGCGGTGATCGGAGCCATGGC
GGGTTATCTCTCGCTATGGTCGATTTACTGGCTATTTAAACTGCTGACAGGTAAAGAAGGCATGGGTTATGGCGATTTCA
AATTGCTCGCCGCACTCGGCACTTGGCTGGGTTGGCAGCAGTTACCTATCATCGTACTGCTCTCATCCGTAGTCGGGGTG
ATTTTCGGTTTAATCCAACTGCGCCAGCAGAAAAAAGGTATTGATATGGCCTTTCCGTTTGGCCCTTATCTCGCCATCGC
GGGGTGGTTTGCCTTGCTGTGGGGCGATAAGGTAATTGATTGGTACTTCACCACTTGGGTAGGACAACCGTTATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-124)

Type IV leader peptidase family (Peptidase_A24)

(135-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

98.282

100

0.983
  pilD Vibrio campbellii strain DS40M4

75

98.969

0.742
  pilD Acinetobacter baumannii D1279779

52.941

93.471

0.495
  pilD Acinetobacter nosocomialis M2

52.574

93.471

0.491
  pilD Neisseria gonorrhoeae MS11

44.444

98.969

0.44