Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AAEY34_RS27170 Genome accession   NZ_CP151647
Coordinates   5841441..5842064 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain HN148     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5836441..5847064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEY34_RS27150 (AAEY34_27150) aroB 5837036..5838142 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  AAEY34_RS27155 (AAEY34_27155) aroK 5838192..5838710 (-) 519 WP_003095833.1 shikimate kinase AroK -
  AAEY34_RS27160 (AAEY34_27160) pilQ 5838722..5840866 (-) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  AAEY34_RS27165 (AAEY34_27165) comL/pilP 5840920..5841444 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  AAEY34_RS27170 (AAEY34_27170) pilO 5841441..5842064 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  AAEY34_RS27175 (AAEY34_27175) pilN 5842061..5842657 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  AAEY34_RS27180 (AAEY34_27180) pilM 5842657..5843721 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  AAEY34_RS27185 (AAEY34_27185) - 5843906..5846377 (+) 2472 WP_003095841.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=889222 AAEY34_RS27170 WP_003103268.1 5841441..5842064(-) (pilO) [Pseudomonas aeruginosa strain HN148]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=889222 AAEY34_RS27170 WP_003103268.1 5841441..5842064(-) (pilO) [Pseudomonas aeruginosa strain HN148]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGTTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACTTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401