Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RRV97_RS01665 Genome accession   NZ_CP135440
Coordinates   346421..347551 (-) Length   376 a.a.
NCBI ID   WP_315611767.1    Uniprot ID   A0AA96NEL5
Organism   Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 341374..353723 346421..347551 within 0


Gene organization within MGE regions


Location: 341374..353723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRV97_RS01640 (RRV97_01640) - 341374..342033 (+) 660 WP_315611764.1 class I SAM-dependent methyltransferase -
  RRV97_RS01645 (RRV97_01645) mutM 342078..342905 (+) 828 WP_315612221.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  RRV97_RS01650 (RRV97_01650) - 342970..343755 (+) 786 WP_315611765.1 1-acylglycerol-3-phosphate O-acyltransferase -
  RRV97_RS01655 (RRV97_01655) - 343945..344682 (+) 738 WP_003747251.1 redoxin family protein -
  RRV97_RS01660 (RRV97_01660) - 344935..346341 (+) 1407 WP_315611766.1 dihydrolipoyl dehydrogenase -
  RRV97_RS01665 (RRV97_01665) pilU 346421..347551 (-) 1131 WP_315611767.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RRV97_RS01670 (RRV97_01670) - 347755..348456 (+) 702 WP_315611768.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -
  RRV97_RS01675 (RRV97_01675) lepA 348589..350382 (+) 1794 WP_049348368.1 translation elongation factor 4 -
  RRV97_RS01680 (RRV97_01680) lepB 350382..351401 (+) 1020 WP_315611769.1 signal peptidase I -
  RRV97_RS01685 (RRV97_01685) - 351456..352334 (-) 879 WP_315611770.1 DMT family transporter -
  RRV97_RS01690 (RRV97_01690) - 352570..353109 (+) 540 WP_036490459.1 gamma carbonic anhydrase family protein -
  RRV97_RS01695 (RRV97_01695) - 353124..353723 (+) 600 WP_315611771.1 Maf family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42469.62 Da        Isoelectric Point: 5.6785

>NTDB_id=886618 RRV97_RS01665 WP_315611767.1 346421..347551(-) (pilU) [Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055]
MFDENTLGAKEELFTWLRHMNKYKGSDLFITTNFPPAMKVDGKIMRLTDEPLSAEKCMEIAFSIMSSKQIEEFSTTNECN
FAISLPDTSRFRVNAMVQRGATALVFRSITSNIPKFETLNLPPILKDIALRKRGLVIFVGGTGSGKSTSLASLIDYRNEN
SFDHIITIEDPIEFIHQHKNCIITQREIGVDTENWPIALKNTLRQAPDVILIGEIRDRETMDYAISFAETGHLCMATLHA
NSTNQALDRIINFFPEERRNQLLTDLSLNLQAFISQRLIPRESGRGRVAAVEVLLNSPIIADLIQKGEVHNIKEMMKKST
GMGMITFDQALYDLYENGDISYQDAIKNADSAHDLRLDIQLRSRRAQNAGPDLELI

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=886618 RRV97_RS01665 WP_315611767.1 346421..347551(-) (pilU) [Neisseria sp. DTU_2021_1001991_1_SI_NGA_ILE_055]
ATGTTTGACGAAAATACTCTGGGCGCAAAAGAAGAATTGTTCACTTGGCTACGCCACATGAACAAATACAAAGGCTCCGA
CCTTTTCATTACCACCAATTTCCCGCCGGCCATGAAAGTGGACGGCAAAATCATGCGGCTTACCGATGAGCCGTTAAGTG
CTGAAAAATGCATGGAAATCGCTTTTTCGATTATGTCTTCCAAACAAATCGAAGAATTTTCTACCACCAACGAATGCAAT
TTCGCCATCAGCCTGCCCGATACCAGCCGCTTCCGTGTCAATGCCATGGTTCAACGCGGCGCAACTGCTTTGGTGTTCCG
CTCTATTACCAGCAATATCCCTAAATTTGAAACGCTCAATCTGCCGCCGATTCTGAAAGACATCGCCCTCAGAAAACGCG
GTCTGGTTATTTTTGTCGGTGGTACAGGTTCGGGCAAGTCCACTTCCCTCGCTTCCCTTATCGACTATCGAAACGAAAAC
AGCTTCGACCACATTATCACCATCGAAGACCCGATTGAATTTATCCACCAACATAAAAACTGCATCATCACCCAACGCGA
AATCGGCGTAGATACCGAAAATTGGCCGATTGCGCTGAAAAATACCCTGCGCCAAGCGCCGGATGTTATTCTGATTGGCG
AAATCCGCGACCGCGAGACCATGGACTACGCCATTTCCTTCGCGGAAACCGGCCATTTGTGTATGGCGACACTGCATGCC
AACAGCACCAACCAAGCGCTCGACCGCATCATCAACTTCTTCCCCGAAGAGCGCCGTAACCAATTGCTAACCGATTTATC
GCTCAACCTGCAAGCCTTTATTTCACAACGACTGATTCCCCGTGAAAGCGGCCGGGGTCGTGTGGCGGCGGTCGAAGTTT
TGCTCAATTCTCCGATTATTGCCGACTTGATTCAAAAAGGTGAAGTCCACAATATCAAAGAAATGATGAAAAAATCAACC
GGCATGGGCATGATCACTTTTGACCAAGCCTTGTATGATTTGTATGAAAACGGCGATATCAGCTATCAAGATGCCATTAA
AAATGCCGACTCCGCTCATGACTTGCGCTTAGACATTCAATTACGAAGCCGTCGTGCGCAAAATGCCGGTCCGGATTTAG
AATTGATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-259)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.795

93.617

0.569
  pilU Acinetobacter baylyi ADP1

57.434

91.223

0.524
  pilU Vibrio cholerae strain A1552

53.203

95.479

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.544

88.564

0.386
  pilT Acinetobacter nosocomialis M2

42.901

86.17

0.37
  pilT Acinetobacter baumannii D1279779

42.901

86.17

0.37
  pilT Acinetobacter baumannii strain A118

42.901

86.17

0.37
  pilT Legionella pneumophila strain Lp02

42.284

86.17

0.364
  pilT Legionella pneumophila strain ERS1305867

42.284

86.17

0.364
  pilT Acinetobacter baylyi ADP1

41.768

87.234

0.364