Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RHP74_RS17115 Genome accession   NZ_CP134498
Coordinates   3740492..3741601 (-) Length   369 a.a.
NCBI ID   WP_200666287.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20052     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3735492..3746601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP74_RS17110 (RHP74_17110) - 3736506..3740366 (-) 3861 WP_311095928.1 hypothetical protein -
  RHP74_RS17115 (RHP74_17115) pilU 3740492..3741601 (-) 1110 WP_200666287.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHP74_RS17120 (RHP74_17120) - 3741723..3744260 (+) 2538 WP_288112611.1 PAS domain-containing protein -
  RHP74_RS17125 (RHP74_17125) - 3744327..3744602 (+) 276 WP_007898282.1 peptidylprolyl isomerase -
  RHP74_RS17130 (RHP74_17130) - 3744724..3745659 (-) 936 WP_083376588.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41011.95 Da        Isoelectric Point: 6.0924

>NTDB_id=880539 RHP74_RS17115 WP_200666287.1 3740492..3741601(-) (pilU) [Pseudomonas sp. SG20052]
MEIDALLQILSDRNGSDLYLSTGAPPSARFDGVLKPLTDQPFKPGAVAAIAESIMDAEQRLEFDRELEMNLAISLAGIGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLILFVGATDCGKSTSLAALIDYRNRHSTGHIVTIED
PIEYIHRHKQSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLSNNLKAFVSQRLVRTLDGQRRAAVEVMLGTPTIGDLIRRNQLDELKDIMEKSAEFGMQTFDGAL
YALVMEGAISEEEALKHADSLSNLKLRLKLYGGGPSTANAPIGDWGLVD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=880539 RHP74_RS17115 WP_200666287.1 3740492..3741601(-) (pilU) [Pseudomonas sp. SG20052]
ATGGAAATCGATGCACTGTTGCAGATACTGTCTGACCGGAATGGATCCGATCTCTATCTTTCCACCGGCGCACCACCCAG
TGCGCGGTTCGACGGTGTGCTCAAGCCGCTGACCGACCAGCCATTCAAGCCGGGAGCAGTCGCGGCCATTGCCGAGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCAATCTCCCTGGCGGGTATCGGGCGC
TTTCGGGTCAACATCTTCAAGCAACGCAACGATGTGTCAATCGTGGCACGCAACATCAAGCTCGACATTCCGCGTTTCGA
AGACCTCAAACTACCGGCGGTGCTGCTCGAAACCGTGATGCTCAAACAAGGATTGATACTGTTCGTCGGCGCCACCGATT
GCGGCAAGTCAACCTCTCTCGCGGCGCTGATCGATTACCGCAACCGCCACAGCACTGGCCATATCGTCACCATCGAAGAC
CCGATCGAGTACATCCATCGGCACAAGCAGTCGATCATCAACCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTTCATGC
TGCCCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTACTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCACG
CGCTGGCGTTTGCCGATACGGGCCATCTGGTGATCTCGACGTTGCACGCGCACAACGCCCATCAGGCGCTGGACCGCATC
ATCAATTTCTTTCCCGAAGAGCGGCGGACGCAGTTGCTGCATGACTTGAGCAACAATCTGAAAGCGTTTGTTTCCCAACG
ATTGGTGCGGACCCTTGACGGTCAGCGCAGGGCGGCAGTGGAAGTGATGTTGGGAACACCCACGATCGGTGACTTGATAA
GGCGCAATCAACTGGACGAACTTAAAGACATCATGGAAAAGTCCGCAGAGTTCGGTATGCAGACGTTCGATGGCGCACTG
TATGCACTGGTGATGGAAGGCGCAATCAGTGAGGAAGAGGCGCTAAAACATGCGGACTCGCTGAGCAATCTGAAGTTGCG
CCTCAAGCTGTATGGCGGTGGGCCTTCGACCGCCAATGCGCCCATTGGGGACTGGGGGTTAGTGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.84

95.122

0.531
  pilU Acinetobacter baylyi ADP1

50.82

99.187

0.504
  pilU Vibrio cholerae strain A1552

50.543

99.729

0.504
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.456

95.122

0.385
  pilT Vibrio cholerae strain A1552

40.456

95.122

0.385
  pilT Pseudomonas aeruginosa PAK

39.826

93.225

0.371
  pilT Legionella pneumophila strain Lp02

41.018

90.515

0.371
  pilT Legionella pneumophila strain ERS1305867

41.018

90.515

0.371
  pilT Pseudomonas stutzeri DSM 10701

39.535

93.225

0.369
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.233

91.87

0.36