Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PszF2a_RS04900 Genome accession   NZ_AP024722
Coordinates   1033134..1034354 (+) Length   406 a.a.
NCBI ID   WP_125877162.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1028134..1039354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PszF2a_RS04875 (PszF2a_09580) - 1028616..1029359 (-) 744 WP_245002918.1 hypothetical protein -
  PszF2a_RS04880 (PszF2a_09590) - 1029454..1030263 (-) 810 WP_245002919.1 hypothetical protein -
  PszF2a_RS04885 - 1030404..1030856 (-) 453 WP_125877166.1 hypothetical protein -
  PszF2a_RS21525 (PszF2a_09600) pilV 1030872..1031051 (-) 180 WP_125877172.1 type IV pilin protein Machinery gene
  PszF2a_RS04895 (PszF2a_09610) pilB 1031428..1033131 (+) 1704 WP_125877164.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PszF2a_RS04900 (PszF2a_09620) pilC 1033134..1034354 (+) 1221 WP_125877162.1 type II secretion system F family protein Machinery gene
  PszF2a_RS04905 (PszF2a_09630) pilD 1034358..1035224 (+) 867 WP_221293034.1 prepilin peptidase Machinery gene
  PszF2a_RS04910 coaE 1035356..1035964 (+) 609 WP_080977605.1 dephospho-CoA kinase -
  PszF2a_RS04915 (PszF2a_09650) yacG 1035961..1036158 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  PszF2a_RS04920 (PszF2a_09660) - 1036171..1036851 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  PszF2a_RS04925 (PszF2a_09670) - 1036908..1037948 (-) 1041 WP_221293035.1 NADP(H)-dependent aldo-keto reductase -
  PszF2a_RS04930 (PszF2a_09680) rplM 1038363..1038791 (+) 429 WP_003287302.1 50S ribosomal protein L13 -
  PszF2a_RS04935 (PszF2a_09690) rpsI 1038807..1039199 (+) 393 WP_013981948.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44613.68 Da        Isoelectric Point: 9.8649

>NTDB_id=87688 PszF2a_RS04900 WP_125877162.1 1033134..1034354(+) (pilC) [Stutzerimonas stutzeri strain F2a]
MAEKALKTSVFTWEGTDRKGGKVKGELAGQSPALIKAQLRKQGINPLKVRKKAVSLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVAAGNSFAGSLRKKPQYFDDLYCNLVESGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFESVFQGFGAELPAFTMMVVQLSRWLQEWWYIVFFALFIAAFLFK
HAYKRSEKFRDSLDRLMLKLPIVGDILYKSVVARYARTLSTTFAAGVPLVDALDSVAGATGNVVFRNAVAKIRMDVSSGV
QLNFSMRTTGIFPTMAIQMTAIGEESGALDEMLDKLASFYEAEVDNMVDNLTTLMEPLIMAVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=87688 PszF2a_RS04900 WP_125877162.1 1033134..1034354(+) (pilC) [Stutzerimonas stutzeri strain F2a]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTGTTCACCTGGGAAGGCACCGACAGAAAAGGTGGCAAGGTCAAGGGGGAGCT
GGCAGGTCAGAGCCCAGCACTGATAAAGGCGCAGCTCCGCAAGCAGGGTATCAATCCGCTGAAGGTTCGCAAGAAGGCCG
TTTCCTTGTTTGGGGCGGGCAAGAAAATCAAGCCGATGGACATCGCACTGTTCACTCGGCAAATGGCCACCATGATGAAG
GCAGGTGTGCCGCTGCTGCAATCGTTCGACATTATTGGCGAGGGCTTCGATAACCCAAATATGCGCAAGCTGGTCGATGA
GATCAAGCAAGAGGTGGCGGCAGGCAACAGCTTTGCTGGCTCGCTGCGTAAGAAGCCGCAATATTTCGATGACTTGTATT
GCAACTTAGTCGAGTCTGGAGAGCAGTCCGGTGCGCTCGAAACGTTGCTAGACCGTGTTGCTACCTACAAGGAGAAAACC
GAGGCGCTGAAGGCCAAGATTAAGAAGGCGATGACCTATCCGATTGCGGTTATCGTGGTGGCAATCATCGTATCGGCGAT
TCTCTTGATTAAGGTGGTCCCTCAGTTCGAAAGTGTATTTCAGGGCTTCGGCGCGGAGCTTCCAGCCTTCACCATGATGG
TGGTGCAGTTGTCTCGTTGGCTGCAGGAGTGGTGGTATATCGTATTCTTTGCGTTATTCATTGCCGCCTTTTTATTCAAG
CATGCATATAAGAGGTCGGAGAAATTTCGTGACTCTCTTGATCGCCTTATGCTCAAGCTGCCGATCGTTGGTGACATTCT
CTACAAGTCTGTAGTTGCGCGATATGCTCGCACATTGTCCACGACATTCGCGGCTGGTGTGCCCTTGGTTGATGCGTTGG
ACTCAGTGGCAGGTGCCACGGGCAACGTCGTGTTCCGCAATGCAGTAGCGAAGATTCGTATGGACGTATCGTCTGGTGTG
CAGCTCAATTTCTCCATGCGCACGACCGGCATTTTTCCCACCATGGCTATTCAAATGACCGCTATCGGCGAAGAGTCCGG
TGCGTTGGACGAAATGCTCGACAAACTGGCCAGTTTCTATGAGGCGGAGGTCGATAACATGGTGGATAACCTTACGACCC
TGATGGAACCGCTGATCATGGCCGTGCTCGGGGTTCTGGTCGGTGGCTTGATCATCGCGATGTATCTGCCGATCTTCCAG
TTGGGTAGTGTGGTCGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.716

99.754

0.825
  pilC Acinetobacter baylyi ADP1

63.547

100

0.635
  pilC Acinetobacter baumannii D1279779

62.469

99.754

0.623
  pilC Legionella pneumophila strain ERS1305867

54.798

97.537

0.534
  pilG Neisseria gonorrhoeae MS11

45.025

99.015

0.446
  pilG Neisseria meningitidis 44/76-A

45.025

99.015

0.446
  pilC Vibrio cholerae strain A1552

43.35

100

0.434
  pilC Vibrio campbellii strain DS40M4

41

98.522

0.404

Multiple sequence alignment