Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PszF2a_RS04895 Genome accession   NZ_AP024722
Coordinates   1031428..1033131 (+) Length   567 a.a.
NCBI ID   WP_125877164.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1026428..1038131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PszF2a_RS04870 (PszF2a_09570) - 1027723..1028619 (-) 897 WP_221293033.1 ABC transporter ATP-binding protein -
  PszF2a_RS04875 (PszF2a_09580) - 1028616..1029359 (-) 744 WP_245002918.1 hypothetical protein -
  PszF2a_RS04880 (PszF2a_09590) - 1029454..1030263 (-) 810 WP_245002919.1 hypothetical protein -
  PszF2a_RS04885 - 1030404..1030856 (-) 453 WP_125877166.1 hypothetical protein -
  PszF2a_RS21525 (PszF2a_09600) pilV 1030872..1031051 (-) 180 WP_125877172.1 type IV pilin protein Machinery gene
  PszF2a_RS04895 (PszF2a_09610) pilB 1031428..1033131 (+) 1704 WP_125877164.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PszF2a_RS04900 (PszF2a_09620) pilC 1033134..1034354 (+) 1221 WP_125877162.1 type II secretion system F family protein Machinery gene
  PszF2a_RS04905 (PszF2a_09630) pilD 1034358..1035224 (+) 867 WP_221293034.1 prepilin peptidase Machinery gene
  PszF2a_RS04910 coaE 1035356..1035964 (+) 609 WP_080977605.1 dephospho-CoA kinase -
  PszF2a_RS04915 (PszF2a_09650) yacG 1035961..1036158 (+) 198 WP_041771656.1 DNA gyrase inhibitor YacG -
  PszF2a_RS04920 (PszF2a_09660) - 1036171..1036851 (-) 681 WP_013981946.1 energy-coupling factor ABC transporter permease -
  PszF2a_RS04925 (PszF2a_09670) - 1036908..1037948 (-) 1041 WP_221293035.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62203.14 Da        Isoelectric Point: 5.4633

>NTDB_id=87687 PszF2a_RS04895 WP_125877164.1 1031428..1033131(+) (pilB) [Stutzerimonas stutzeri strain F2a]
MNENAPLGGLARQLVLTGQLDEKVAQQAQQQALRSQVPLVTWLVQNKLVKSRALVDIAGEQFGVPFFDLGTLDKETQPKE
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDKLGEAIEKFFESATSSLEGLNDSELD
GLDIEVAGDAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSIDFRVNTLPTLWGEKIVMRILDSSSAKMGIDALGYEDGQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKQEVDVPRDVLLSEGFP
EARIGSFKLYGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARRDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=87687 PszF2a_RS04895 WP_125877164.1 1031428..1033131(+) (pilB) [Stutzerimonas stutzeri strain F2a]
ATGAACGAAAATGCGCCCCTTGGCGGTTTGGCCCGCCAGCTGGTCCTGACTGGCCAGCTCGACGAAAAAGTTGCCCAGCA
AGCTCAGCAGCAGGCTTTGCGGAGTCAGGTTCCGTTGGTTACCTGGCTGGTGCAGAACAAGCTGGTCAAAAGTCGGGCGC
TTGTCGATATCGCCGGTGAGCAGTTTGGTGTTCCGTTCTTTGATTTAGGAACGCTGGACAAAGAAACCCAGCCGAAGGAA
CTGGTCAGTGAGAAGCTGGTGCGCCAGCACCGCGCTCTGCCGTTGCAGCGACGAGGCAACAAGCTTTATGTGGCGGTGTC
CGATCCAACCAACCATCAGGCGATAAGTGATATTCAGTTCAATACGGGGCTTTCCGTTGAGGCTGTGCTGGTTGAGGACG
ACAAGCTCGGTGAGGCCATCGAAAAGTTCTTCGAGTCGGCGACCTCGTCCCTCGAAGGATTGAACGATTCCGAGTTGGAT
GGCTTGGATATCGAAGTGGCCGGCGACGCCAAGGAGGACGATGCGGCGCATAACGAAGCCGATGATGCGCCAGTGGTGCG
GTTTGTTAACAAGATGCTGCTCGATGCAATCAAGGGTGGCTCGTCCGATTTGCACTTCGAGCCCTATGAGAAGACCTATC
GGGTGCGCTTTCGTACGGATGGCATGTTGCACGAGGTTGCCCGTCCACCGGTCCAGCTGGTCAGCCGTATCTCTGCGCGA
TTGAAAGTAATGGCCAGTCTGGATATCGCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGAAGGTCTCGAGCAC
CAAGTCGATCGATTTTCGCGTGAATACGCTACCCACTCTGTGGGGCGAGAAGATCGTTATGCGAATCTTGGATTCTTCCA
GTGCCAAGATGGGTATTGATGCGCTGGGGTATGAGGATGGCCAGAAGGAGCTCTATCTAAGCGCGCTGAAGCAGCCGCAA
GGGATGATTCTGGTTACCGGTCCGACAGGCTCGGGCAAGACCGTCTCGCTTTATACCGGCCTGAATATTCTCAATACGGT
CGATGTGAATATCTCCACTGCCGAGGATCCGGTAGAGATCAATTTGGAAGGTATCAATCAGGTCAACGTAAATCCGAAGC
AGGGCATGGACTTCGCCCAGGCGCTTCGAGCGTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGTGAGATTCGAGAT
CTCGAAACCGCCGAGATTGCCATCAAGGCAGCGCAGACCGGGCACATGGTGATGTCGACTCTGCATACCAATAGCGCTGC
GGAAACGCTTACTCGCTTGCGCAACATGGGGGTGCCGGCATTCAACATTGCCACATCGGTCAACCTGATCATTGCTCAAC
GGCTGGCTCGTAAGCTGTGCCCCAGCTGTAAACAAGAAGTCGATGTGCCGCGAGATGTTCTGCTCAGCGAAGGTTTTCCT
GAAGCGCGAATCGGTTCCTTCAAGCTTTACGGGCCGGTGGGGTGCGATAGCTGCAAAGGAGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCGGCCCTGCAGCGGCTTATCATGGAAGAAGGCAATTCCATCGATATCGCCTTGC
AGGCGCGTAGAGATGGGTTCAATGACCTGCGCACTTCGGGTCTGGTTAAAGCCATGAACGGCGTCACCAGCCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.609

99.118

0.591
  pilB Acinetobacter baylyi ADP1

58.333

99.471

0.58
  pilB Legionella pneumophila strain ERS1305867

54.991

100

0.554
  pilB Vibrio cholerae strain A1552

51.636

97.002

0.501
  pilB Vibrio campbellii strain DS40M4

50.181

97.707

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

51.391

95.062

0.489
  pilF Neisseria gonorrhoeae MS11

50.645

95.767

0.485
  pilF Thermus thermophilus HB27

38.366

99.295

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.566

93.474

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362

Multiple sequence alignment