Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RHM59_RS20910 Genome accession   NZ_CP133783
Coordinates   4651398..4652615 (-) Length   405 a.a.
NCBI ID   WP_322285315.1    Uniprot ID   -
Organism   Pseudomonas sp. MH10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4646398..4657615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM59_RS20875 (RHM59_20875) - 4647433..4647606 (-) 174 WP_322285310.1 DUF3094 family protein -
  RHM59_RS20880 (RHM59_20880) - 4647643..4648152 (-) 510 WP_322166100.1 MOSC domain-containing protein -
  RHM59_RS20885 (RHM59_20885) - 4648149..4648778 (-) 630 WP_322166099.1 DUF1780 domain-containing protein -
  RHM59_RS20890 (RHM59_20890) - 4648978..4649676 (+) 699 WP_322285311.1 energy-coupling factor ABC transporter permease -
  RHM59_RS20895 (RHM59_20895) yacG 4649704..4649907 (-) 204 WP_322285312.1 DNA gyrase inhibitor YacG -
  RHM59_RS20900 (RHM59_20900) coaE 4649904..4650527 (-) 624 WP_322285313.1 dephospho-CoA kinase -
  RHM59_RS20905 (RHM59_20905) pilD 4650524..4651396 (-) 873 WP_322285314.1 A24 family peptidase Machinery gene
  RHM59_RS20910 (RHM59_20910) pilC 4651398..4652615 (-) 1218 WP_322285315.1 type II secretion system F family protein Machinery gene
  RHM59_RS20915 (RHM59_20915) pilB 4652618..4654318 (-) 1701 WP_322285316.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM59_RS20920 (RHM59_20920) - 4654537..4654974 (+) 438 WP_416195104.1 pilin -
  RHM59_RS20930 (RHM59_20930) nadC 4655240..4656088 (-) 849 WP_322166091.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44216.25 Da        Isoelectric Point: 10.0401

>NTDB_id=876760 RHM59_RS20910 WP_322285315.1 4651398..4652615(-) (pilC) [Pseudomonas sp. MH10]
MATKAVKVSVYAWEGKDKKGAKLTGELNGHNPALVKALLRKQGITPIKVRKKTASFFGEGKKIKPIDIAFFTRQMATMMK
AGVPLLQSFDIIGESSDNPRMRTLVNEIKQEVAAGNSFASSLRKKPEYFDDLYCSLVDAGEQAGALEALLERVATYKEKT
EMLKAKIKKAMTYPIAVVIVALIVSSILLMKVVPQFQTVFSSFGAKLPAFTLMVIGLSDLLQNWWYIVLAGVVLSIFCFK
RAYKTSQKFRDAIDRGLLKVPIVGPLIYKSSVARYARTLSTTFAAGVPLVEALESVASATGNVVFRNAVNRIKLDVSTGM
QLNFSMRTTGVFPTLAIQMTAIGEESGALDSMLDKVATFYEDEVDGMVDNMTALMEPFIMAVLGVVVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=876760 RHM59_RS20910 WP_322285315.1 4651398..4652615(-) (pilC) [Pseudomonas sp. MH10]
ATGGCGACCAAGGCAGTCAAAGTGAGTGTTTACGCTTGGGAAGGCAAGGACAAAAAGGGTGCAAAACTGACCGGAGAACT
AAACGGGCACAATCCAGCCCTGGTCAAAGCACTGTTGCGCAAGCAGGGCATCACACCGATCAAGGTGCGTAAAAAAACCG
CCTCATTTTTTGGTGAAGGCAAAAAGATAAAGCCCATTGATATTGCGTTTTTCACACGGCAGATGGCGACCATGATGAAA
GCAGGGGTTCCGCTATTGCAATCTTTCGACATCATCGGCGAAAGCTCTGATAACCCGCGAATGAGAACCTTAGTCAACGA
AATCAAACAAGAAGTGGCGGCAGGTAACAGCTTCGCGTCGTCGTTACGGAAAAAGCCAGAATACTTCGATGATCTTTATT
GCAGTTTGGTAGACGCAGGCGAGCAAGCGGGTGCATTGGAAGCCTTGCTGGAAAGGGTAGCGACCTATAAAGAAAAGACA
GAAATGCTCAAGGCAAAAATAAAGAAGGCCATGACCTACCCAATCGCGGTAGTTATCGTAGCCTTGATTGTGTCGTCAAT
TCTACTAATGAAAGTTGTTCCTCAATTCCAGACCGTTTTTTCAAGCTTTGGCGCAAAGCTACCCGCCTTTACGTTGATGG
TCATTGGCCTTTCAGACCTGTTACAGAATTGGTGGTATATCGTTTTAGCGGGAGTTGTTCTTTCAATCTTTTGCTTTAAA
CGAGCCTATAAAACTTCTCAAAAATTCCGGGACGCGATAGACCGAGGCTTACTCAAAGTCCCCATTGTCGGCCCATTGAT
CTACAAATCATCCGTCGCCCGCTACGCCCGTACTTTGTCGACCACGTTCGCGGCTGGCGTACCGCTGGTCGAAGCACTGG
AATCTGTCGCCAGCGCCACGGGGAATGTGGTGTTCAGAAACGCAGTGAACCGCATAAAACTCGACGTATCTACCGGCATG
CAACTGAACTTTTCAATGCGCACCACCGGAGTGTTTCCCACATTAGCGATTCAAATGACAGCCATTGGTGAAGAGTCAGG
CGCCTTGGATTCGATGCTCGATAAAGTCGCAACCTTTTACGAAGACGAAGTTGACGGCATGGTCGATAACATGACCGCGC
TGATGGAGCCCTTCATCATGGCAGTCCTCGGCGTAGTAGTCGGTGGCCTCGTCATCGCGATGTACCTCCCCATCTTCCAG
CTCGGAAACGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

76.543

100

0.765
  pilC Acinetobacter baylyi ADP1

59.169

100

0.598
  pilC Acinetobacter baumannii D1279779

58.765

100

0.588
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilG Neisseria meningitidis 44/76-A

42.184

99.506

0.42
  pilG Neisseria gonorrhoeae MS11

42.184

99.506

0.42
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Vibrio cholerae strain A1552

41.235

100

0.412
  pilC Thermus thermophilus HB27

36.908

99.012

0.365