Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RHM59_RS20915 Genome accession   NZ_CP133783
Coordinates   4652618..4654318 (-) Length   566 a.a.
NCBI ID   WP_322285316.1    Uniprot ID   -
Organism   Pseudomonas sp. MH10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4647618..4659318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM59_RS20880 (RHM59_20880) - 4647643..4648152 (-) 510 WP_322166100.1 MOSC domain-containing protein -
  RHM59_RS20885 (RHM59_20885) - 4648149..4648778 (-) 630 WP_322166099.1 DUF1780 domain-containing protein -
  RHM59_RS20890 (RHM59_20890) - 4648978..4649676 (+) 699 WP_322285311.1 energy-coupling factor ABC transporter permease -
  RHM59_RS20895 (RHM59_20895) yacG 4649704..4649907 (-) 204 WP_322285312.1 DNA gyrase inhibitor YacG -
  RHM59_RS20900 (RHM59_20900) coaE 4649904..4650527 (-) 624 WP_322285313.1 dephospho-CoA kinase -
  RHM59_RS20905 (RHM59_20905) pilD 4650524..4651396 (-) 873 WP_322285314.1 A24 family peptidase Machinery gene
  RHM59_RS20910 (RHM59_20910) pilC 4651398..4652615 (-) 1218 WP_322285315.1 type II secretion system F family protein Machinery gene
  RHM59_RS20915 (RHM59_20915) pilB 4652618..4654318 (-) 1701 WP_322285316.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RHM59_RS20920 (RHM59_20920) - 4654537..4654974 (+) 438 WP_416195104.1 pilin -
  RHM59_RS20930 (RHM59_20930) nadC 4655240..4656088 (-) 849 WP_322166091.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RHM59_RS20935 (RHM59_20935) - 4656419..4658662 (+) 2244 WP_322285318.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62468.80 Da        Isoelectric Point: 5.9204

>NTDB_id=876761 RHM59_RS20915 WP_322285316.1 4652618..4654318(-) (pilB) [Pseudomonas sp. MH10]
MTDVALTGLAKQLAVAGLLNEKVAQDAYQKAQRSKISLVSYLVQNKLLKSIVLAEIASEQFGVPFLDLSTLDKEGQPKGL
VSEKLIRQHHVLPLWRRGNKLFVGISDPTNHQAVTDVQFSTGLNTEAILVEDDKLTDAIEKFFEGANAGLGEMADVDLDS
LDIDTSDDKKQDTIAGQDADDAPVVRFVNKMLLDAIKLGSSDLHFEPYEKTYRVRLRTDGILHEVAKPPVQLASRIAARL
KVMASLDISERRRPQDGRIKLRISKTKAIDFRVNTLPTLWGEKIVMRILDPSTAQMGIDALGYEPDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLQHMGVAAFNIATAINLIIAQRLARKLCPLCKKEADIPRETLIREGFPE
EKIGTFKIYSPVGCDHCNNGYKGRVGIYEVVKSTPELQRLIMEEGNSMDIAVQMRKDGFNDLRTSGLMKAMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=876761 RHM59_RS20915 WP_322285316.1 4652618..4654318(-) (pilB) [Pseudomonas sp. MH10]
ATGACTGATGTCGCTTTAACAGGTTTGGCCAAGCAACTGGCGGTAGCGGGGCTGCTAAATGAAAAGGTCGCTCAAGACGC
TTATCAGAAAGCTCAGCGCAGCAAAATATCGCTGGTTAGCTACCTGGTGCAGAATAAACTGCTTAAAAGCATCGTTTTGG
CTGAGATTGCTTCAGAGCAATTTGGTGTGCCCTTTCTAGATTTAAGCACGCTTGATAAAGAGGGACAGCCCAAGGGGTTA
GTAAGCGAAAAATTGATTCGCCAACACCATGTCTTGCCGTTATGGCGACGGGGCAATAAATTATTTGTAGGAATTTCAGA
CCCTACCAACCACCAAGCTGTCACCGATGTTCAGTTCAGCACCGGCCTCAACACTGAAGCTATCTTGGTAGAGGATGACA
AATTAACTGACGCTATAGAAAAATTTTTCGAGGGTGCCAACGCCGGTTTAGGCGAAATGGCGGATGTCGACCTTGATAGT
CTCGACATAGACACTTCGGATGACAAGAAACAAGACACCATCGCCGGGCAAGATGCCGACGATGCACCCGTAGTTCGTTT
CGTTAATAAAATGCTTTTGGACGCCATTAAGCTGGGCTCATCGGACCTGCACTTTGAACCATATGAAAAGACTTACCGGG
TGCGTCTACGCACCGACGGCATTCTTCACGAGGTAGCAAAGCCGCCCGTTCAGTTGGCCAGCAGGATCGCTGCGCGCTTA
AAGGTCATGGCTAGCCTGGATATCTCAGAACGCCGCCGCCCACAAGACGGCCGGATCAAATTGCGGATTTCAAAAACCAA
GGCGATCGACTTTCGAGTCAATACCTTGCCCACCTTGTGGGGCGAAAAGATCGTGATGCGGATTCTTGATCCGTCCACCG
CACAGATGGGAATTGATGCACTCGGCTATGAGCCCGATCAAAAAGAGCTGTATCTGGCGGCGCTTAAACAGCCCCAGGGC
ATGATATTAGTTACCGGACCTACCGGTTCAGGAAAAACCGTTTCGCTTTACACCGGCCTGAATATTCTCAACACCGTTGA
CATCAACATTTCCACTGCAGAAGACCCGGTGGAGATCAACCTGGAAGGTATCAATCAGGTTAACGTCAACCCACGCCAAG
GCATGGACTTTTCACAGGCATTGCGCGCCTTCTTGCGTCAAGACCCGGATGTAATCATGGTCGGGGAAATTCGCGACTTG
GAAACGGCGGAAATTGCCATCAAGGCCTCGCAAACAGGCCACATGGTGTTGTCTACGTTACACACCAACAGCGCAGCTGA
AACCCTGACTCGCCTTCAGCACATGGGCGTGGCTGCATTCAATATTGCTACCGCTATTAACCTGATCATCGCTCAACGTT
TGGCACGAAAATTGTGTCCCTTGTGCAAGAAGGAAGCAGATATACCCCGTGAAACGCTGATCAGGGAAGGGTTTCCCGAA
GAAAAAATTGGCACGTTTAAGATTTACTCTCCGGTTGGCTGTGACCATTGCAACAATGGCTATAAAGGCCGGGTCGGGAT
TTATGAGGTGGTGAAAAGCACCCCCGAGCTGCAACGGCTCATCATGGAGGAAGGTAACTCGATGGATATCGCTGTTCAAA
TGCGTAAGGACGGCTTTAACGACCTGCGTACGTCGGGATTGATGAAGGCGATGCAAGGTGTCACCAGCCTCGAAGAAATC
AACCGTGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-144)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.57

100

0.578
  pilB Acinetobacter baylyi ADP1

57.093

100

0.576
  pilB Legionella pneumophila strain ERS1305867

55.654

100

0.557
  pilB Vibrio cholerae strain A1552

51.068

99.293

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.177

100

0.502
  pilB Vibrio campbellii strain DS40M4

49.468

99.647

0.493
  pilF Neisseria gonorrhoeae MS11

47.247

99.47

0.47
  pilF Thermus thermophilus HB27

37.634

98.587

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.937

95.936

0.364