Detailed information NTG0876715

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RHM68_RS13525
• Genome accession: NZ_CP133782
• Coordinates: 2995766..2996875 (-)
• Length: 369 a.a.
• NCBI ID: WP_322215389.1
• Uniprot ID: -
• Organism: Pseudomonas sp. DC1.2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2990766..3001875

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHM68_RS13510 (RHM68_13510)	-	2991542..2992933 (+)	1392	WP_322215383.1	GntP family permease	-
RHM68_RS13515 (RHM68_13515)	-	2992941..2993714 (+)	774	WP_322215385.1	3-hydroxybutyrate dehydrogenase	-
RHM68_RS13520 (RHM68_13520)	-	2993768..2995723 (+)	1956	WP_322215387.1	acetoacetate--CoA ligase	-
RHM68_RS13525 (RHM68_13525)	pilU	2995766..2996875 (-)	1110	WP_322215389.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RHM68_RS13530 (RHM68_13530)	-	2997008..2999545 (+)	2538	WP_322215391.1	PAS domain-containing protein	-
RHM68_RS13535 (RHM68_13535)	-	2999598..2999873 (+)	276	WP_028622750.1	peptidylprolyl isomerase	-
RHM68_RS13540 (RHM68_13540)	-	2999958..3000890 (-)	933	WP_322215395.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40944.88 Da
• Isoelectric Point: 6.4468

>NTDB_id=876715 RHM68_RS13525 WP_322215389.1 2995766..2996875(-) (pilU) [Pseudomonas sp. DC1.2]
MEIDALLRHLASENGSDLYLSTGAPPSARFDGVLKPLDDQPFKPGEIAAIADSIMDAEQRVEFDRDLEMNLAISLSGVGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVRTREGQRRAAVEVMLGTPTIADLIRRNLFSELKGIMEKSGGLGMQTFDAAL
YALVLEGAIDEAEALKHADSVNNLRLRLKLHADAAPAPQAPPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=876715 RHM68_RS13525 WP_322215389.1 2995766..2996875(-) (pilU) [Pseudomonas sp. DC1.2]
ATGGAAATAGATGCGTTGTTGCGACACCTTGCCAGTGAAAATGGCTCTGATCTTTATCTGTCCACTGGCGCACCCCCCAG
TGCACGGTTCGACGGTGTGCTCAAGCCATTGGACGATCAGCCGTTCAAACCGGGGGAAATCGCGGCCATTGCCGATTCCA
TCATGGACGCCGAACAACGCGTTGAGTTCGATCGGGACCTGGAAATGAACCTGGCGATTTCCCTGTCCGGGGTCGGGCGC
TTTCGGGTCAATATCTTCAAACAGCGCAACGACGTATCGATTGTGGCGCGCAACATCAAACTCGACATTCCGCGCTTCGA
AGACCTTAAGTTGCCCGCAGTGCTGCTGGAAACCGTGATGCTCAAGCAGGGGCTGATGCTGTTTGTCGGCGCCACTGACT
CGGGCAAGTCCACGTCCCTGGCAGCGCTGATCGATTACCGTAACCGCCACAGCAGTGGCCATATCATCACCATCGAAGAC
CCGGTGGAATACATCCATCGACACAAGAAGTCGATCATCAATCAGCGAGAAGTTGGCGTTGACACTCGCAGTTTCCATGC
GGCCCTGAAAAATACTCTGCGTCAGGCGCCGGATGTGGTGCTGATTGGCGAAATTCGCGACCGCGAGACCATGGAACATG
CATTGGCGTTCGCGGATACCGGCCATCTGGTGATTTCGACTTTGCACGCTCACAACGCCAATCAGGCGCTGGATCGCATC
ATCAATTTCTTTCCCGAAGAGCGCCGTCCGCAGTTGCTCCACGACCTCGGTAATAACCTCAAGGCGTTTGTTTCACAGCG
GCTGGTGCGCACACGCGAGGGGCAGCGTCGGGCGGCGGTCGAGGTAATGCTCGGGACGCCGACCATCGCGGACCTGATCC
GGCGTAATCTGTTTTCCGAGCTCAAGGGGATCATGGAAAAGTCCGGGGGGCTGGGAATGCAAACGTTCGACGCGGCGCTT
TATGCGTTGGTGCTCGAGGGTGCAATCGATGAGGCGGAGGCGTTGAAGCATGCCGATTCGGTGAATAACTTGCGCTTGAG
ATTGAAGTTGCACGCCGATGCTGCGCCGGCCCCCCAAGCCCCACCCGGTGAGTGGGGGCTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	96.206	0.542
pilU	Acinetobacter baylyi ADP1	52.809	96.477	0.509
pilU	Vibrio cholerae strain A1552	50.857	94.851	0.482
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.503	100	0.39
pilT	Pseudomonas aeruginosa PAK	40.116	93.225	0.374
pilT	Pseudomonas stutzeri DSM 10701	40.116	93.225	0.374
pilT	Vibrio cholerae strain A1552	41.441	90.244	0.374
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.441	90.244	0.374
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363