Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   RHM61_RS02250 Genome accession   NZ_CP133779
Coordinates   471132..471569 (-) Length   145 a.a.
NCBI ID   WP_322249508.1    Uniprot ID   A0AAU0S7C7
Organism   Undibacterium sp. CCC3.4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466132..476569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM61_RS02230 (RHM61_02230) moaC 467302..467805 (+) 504 WP_322249505.1 cyclic pyranopterin monophosphate synthase MoaC -
  RHM61_RS02235 (RHM61_02235) - 467827..469563 (-) 1737 WP_322249506.1 PglL family O-oligosaccharyltransferase -
  RHM61_RS02240 (RHM61_02240) tfpZ 469573..470328 (-) 756 WP_322249507.1 TfpX/TfpZ family type IV pilin accessory protein -
  RHM61_RS02245 (RHM61_02245) comP 470475..470906 (-) 432 WP_416200208.1 pilin Machinery gene
  RHM61_RS02250 (RHM61_02250) pilA 471132..471569 (-) 438 WP_322249508.1 pilin Machinery gene
  RHM61_RS02255 (RHM61_02255) - 472226..472651 (-) 426 WP_322249509.1 pilin -
  RHM61_RS02260 (RHM61_02260) - 472898..473497 (-) 600 WP_322249510.1 FHA domain-containing protein -
  RHM61_RS02265 (RHM61_02265) - 473526..474332 (-) 807 WP_322249511.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  RHM61_RS02270 (RHM61_02270) sucD 474490..475371 (-) 882 WP_322249512.1 succinate--CoA ligase subunit alpha -
  RHM61_RS02275 (RHM61_02275) sucC 475390..476559 (-) 1170 WP_322249513.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 14996.30 Da        Isoelectric Point: 8.1195

>NTDB_id=876686 RHM61_RS02250 WP_322249508.1 471132..471569(-) (pilA) [Undibacterium sp. CCC3.4]
MKSMNMMKKVQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVAEVLGLMEGFKPTVVENLSNNNGSGAGTCTGISG
VTTATVNTSSVTCVDTTGVLTGVGTSKANNVSLSLTPSYSAGAPVTWACTTAAANFRFVPSECRH

Nucleotide


Download         Length: 438 bp        

>NTDB_id=876686 RHM61_RS02250 WP_322249508.1 471132..471569(-) (pilA) [Undibacterium sp. CCC3.4]
ATGAAATCGATGAATATGATGAAAAAAGTACAGCAAGGCTTCACCCTGATCGAATTGATGATTGTTGTTGCAATTATCGG
TATCTTGGCTGCGGTTGCTTTGCCTGCGTACCAAGATTACACAGTGCGCGCCCGCGTTGCTGAGGTGTTGGGCTTGATGG
AAGGCTTCAAGCCTACGGTCGTTGAAAACTTGTCTAACAACAATGGTTCGGGCGCTGGTACTTGTACTGGTATTTCTGGT
GTGACAACAGCGACAGTCAATACTTCCTCAGTTACTTGTGTTGATACAACAGGCGTTCTGACAGGTGTCGGTACATCGAA
AGCTAACAATGTGTCGTTGTCGCTGACTCCATCATACTCGGCTGGTGCGCCAGTCACTTGGGCTTGTACTACAGCAGCAG
CGAATTTCCGTTTCGTTCCTTCGGAATGCCGTCATTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

41.279

100

0.49
  comP Acinetobacter baylyi ADP1

48.276

100

0.483
  pilE Neisseria gonorrhoeae strain FA1090

38.889

100

0.434
  pilE Neisseria gonorrhoeae MS11

38.994

100

0.428
  pilA2 Legionella pneumophila str. Paris

45.522

92.414

0.421
  pilA2 Legionella pneumophila strain ERS1305867

45.522

92.414

0.421
  pilA/pilA1 Eikenella corrodens VA1

38.312

100

0.407
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.013

100

0.393
  pilA/pilAI Pseudomonas stutzeri DSM 10701

35.811

100

0.366