Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   RF679_RS09590 Genome accession   NZ_CP133720
Coordinates   2289869..2290285 (+) Length   138 a.a.
NCBI ID   WP_309480421.1    Uniprot ID   -
Organism   Undibacterium cyanobacteriorum strain 20NA77.5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2284869..2295285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RF679_RS09560 (RF679_09560) - 2284960..2285955 (-) 996 WP_309480415.1 threo-3-hydroxy-L-aspartate ammonia-lyase -
  RF679_RS09565 (RF679_09565) - 2286005..2286742 (-) 738 WP_373921766.1 sulfite exporter TauE/SafE family protein -
  RF679_RS09570 (RF679_09570) - 2286986..2287468 (+) 483 WP_309480417.1 hypothetical protein -
  RF679_RS09575 (RF679_09575) - 2287636..2288130 (+) 495 WP_309480418.1 hypothetical protein -
  RF679_RS09580 (RF679_09580) - 2288180..2288677 (+) 498 WP_309480419.1 hypothetical protein -
  RF679_RS09585 (RF679_09585) - 2288717..2289388 (+) 672 WP_309480420.1 hypothetical protein -
  RF679_RS09590 (RF679_09590) pilA2 2289869..2290285 (+) 417 WP_309480421.1 pilin Machinery gene
  RF679_RS09595 (RF679_09595) - 2290603..2291178 (+) 576 WP_309480422.1 hypothetical protein -
  RF679_RS09600 (RF679_09600) - 2291331..2291969 (+) 639 WP_309480423.1 protein phosphatase CheZ -
  RF679_RS09605 (RF679_09605) - 2292140..2292367 (-) 228 WP_309480424.1 hypothetical protein -
  RF679_RS09610 (RF679_09610) imuA 2292750..2293508 (+) 759 WP_309480425.1 translesion DNA synthesis-associated protein ImuA -
  RF679_RS09615 (RF679_09615) - 2293414..2294934 (+) 1521 WP_309480426.1 DNA polymerase Y family protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14313.42 Da        Isoelectric Point: 8.4704

>NTDB_id=876096 RF679_RS09590 WP_309480421.1 2289869..2290285(+) (pilA2) [Undibacterium cyanobacteriorum strain 20NA77.5]
MKITAKKLHQGFTLIELMIVVAIIGILAAVALPAYKDYTVRAKVTEVVLAASSGKDAVAEHFNYTGTLPSSTEVTIGSQT
TQYVSSVAWNGSVITATATGESKINGSTITLTPSASGDILNWTCSGTIGAAYRPASCK

Nucleotide


Download         Length: 417 bp        

>NTDB_id=876096 RF679_RS09590 WP_309480421.1 2289869..2290285(+) (pilA2) [Undibacterium cyanobacteriorum strain 20NA77.5]
ATGAAAATCACTGCAAAGAAGTTACATCAGGGTTTTACCCTGATTGAATTGATGATCGTTGTTGCGATTATTGGTATTTT
GGCGGCTGTCGCTTTGCCTGCATACAAGGATTACACAGTTCGTGCCAAAGTGACTGAAGTAGTTTTGGCGGCATCTTCTG
GTAAAGATGCTGTCGCAGAACATTTTAATTACACAGGAACGCTTCCTTCGTCGACGGAAGTCACTATTGGATCTCAAACC
ACTCAATACGTTTCTAGTGTTGCTTGGAATGGCTCTGTAATTACAGCGACTGCCACTGGTGAATCAAAAATTAATGGCAG
TACAATTACTTTAACACCATCTGCATCAGGCGATATTTTGAACTGGACTTGCTCTGGCACTATCGGTGCTGCGTATCGTC
CTGCCAGCTGTAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)

Pilin (bacterial filament) (Pilin)

(40-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

50.735

98.551

0.5
  pilA2 Legionella pneumophila strain ERS1305867

50.735

98.551

0.5
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.364

100

0.493
  pilA Ralstonia pseudosolanacearum GMI1000

38.372

100

0.478
  comP Acinetobacter baylyi ADP1

44.056

100

0.457
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.754

100

0.428
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.876

99.275

0.406
  pilE Neisseria gonorrhoeae strain FA1090

44.355

89.855

0.399
  pilE Neisseria gonorrhoeae MS11

44

90.58

0.399
  pilA/pilA1 Eikenella corrodens VA1

39.552

97.101

0.384